NCAM-1/CD56 Antibody (735) - Azide and BSA Free

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, IHC, IP, CyTOF-ready
• Clone: 735
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

NCAM-1/CD56 Antibody (735) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NCAM-1/CD56 Antibody (735) - Azide and BSA Free (NBP2-52710) is a recombinant monoclonal antibody validated for use in IHC, WB, ELISA, Flow and IP. Anti-NCAM-1/CD56 Antibody: Cited in 3 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Homopolymers of alpha 2,8-linked sialic acid of Escherichia coli K1.
• Specificity: Recognises Polysialic acid (PSA), a carbohydrate polymer attached to the neural cell adhesion molecule (NCAM), which shares molecular epitopes with homopolymers of (alpha)2,8-linked sialic acid of Escherichia coli K1.
• Isotype: IgG2a Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • Immunoprecipitation 1:10 - 1:500
  • Western Blot
• Application Notes: This antibody is Cytof ready.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for NCAM-1/CD56 Antibody (735) - Azide and BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCAM-1/CD56 Antibody (NBP2-52710)(3)

Publications using NBP2-52710

• Muller-Miny L, Thiel K, Meinert S et al. Association of polysialic acid serum levels with schizophrenia spectrum and bipolar disorder-related structural brain changes and hospitalization Scientific reports 2023-02-06 [PMID: 36747002] (ELISA, Human)
• Rivero O, Alhama-Riba J, Ku HP Et al. Haploinsufficiency of the Attention-Deficit/Hyperactivity Disorder Risk Gene St3gal3 in Mice Causes Alterations in Cognition and Expression of Genes Involved in Myelination and Sialylation Frontiers in genetics 2021-09-28 [PMID: 34650588] (WB, Mouse)
• Nagaraj, V, Theis, T Et al. Application of Antibodies to Neuronally Expressed Nogo-A Increases Neuronal Survival and Neurite Outgrowth. Int J Mol Sci 2020-07-30 [PMID: 32751444] (IF/IHC, Mouse)

Bioinformatics

• Gene Symbol: NCAM1