NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

Western Blot: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Western blot analysis of human brain tissue lysate using NCAM-1/CD56 antibody (123A8 (same as 56C04)).

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human colon stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04)).

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human Pancreas stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04))

Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Formalin-fixed, paraffin-embedded human thyroid stained with NCAM-1/CD56 Antibody (123A8 (same as 56C04)). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Imaging Mass Cytometry: NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free [NBP2-34518] - Image of human cutaneous squamous cell carcinoma tumor stained with Anti-NCAM-1/CD56 Azide/BSA Free Antibody [123A8] (in red). IMC image submitted by a verified customer review.

Formalin-fixed, paraffin-embedded human thyroid stained with NCAM-1/CD56 antibody (123A8 (same as 56C04)). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC, MS
• Clone: 123A8 (same as 56C04)
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1.0 mg/ml

NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free Summary

• Description: 1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-34279).
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: Membrane preparation of a small cell lung carcinoma
• Localization: Cell surface
• Marker: Neuronal Cell Marker
• Specificity: This monoclonal antibody reacts with an extracellular domain (close to transmembrane) of CD56/NCAM. Three isoforms of neural cell adhesion molecule (NCAM) are produced by differential splicing of the RNA transcript from a single gene. The 135kDa isoform is the basic molecule, which is glycosylated or sialylated to produce the mature species. Anti-CD56 recognizes two proteins of the neural cell adhesion molecule, the basic molecule expressed on most neuroectodermally derived tissues and neoplasms (e.g. retinoblastoma, medulloblastomas, astrocytomas, neuroblastomas, and small cell carcinomas). It is also expressed on some mesodermally derived tumors (rhabdomyosarcoma). Anti-CD56 plays an important role in the diagnosis of nodal and nasal NK/T-cell lymphomas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: NCAM1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Imaging Mass Cytometry
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.

Packaging, Storage & Formulations

• Storage: Store at -20 to -80C. Avoid freeze-thaw cycles.
• Buffer: 10 mM PBS
• Preservative: No Preservative
• Concentration: 1.0 mg/ml
• Purity: Protein A or G purified

Alternate Names for NCAM-1/CD56 Antibody (123A8 (same as 56C04)) - Azide and BSA Free

• CD56 / NCAM-1
• CD56 antigen
• CD56
• MSK39
• NCAM1
• N-CAM-1
• NCAM-1
• NCAMantigen recognized by monoclonal 5.1H11
• neural cell adhesion molecule 1
• neural cell adhesion molecule, NCAM

Background

NCAM, as a member of the immunoglobulin superfamily of adhesion molecules is characterized by several immunoglobulin (Ig) like domains. The extracellular part of NCAM consists of five of these Ig domains and two fibronectin type III homology regions. NCAM is encoded by a single copy gene composed of 26 exons. However, at least 20-30 distinct isoforms can be generated by alternative splicing and by posttranslational modifications, such as sialylation. During sialylation, polysialic acid (PSA) carbohydrates are attached to the extracellular part of NCAM. Through its extracellular region, NCAM mediates homophilic interactions. In addition, NCAM can also undergo heterophilic interactions by binding extracellular matrix components, such as laminin, or other cell adhesion molecules, such as integrins. NCAM is expressed on most neuroectodermal derived cell lines, tissues and neoplasm such as retinoblastoma, medulloblastoma, astrocytomas and neuroblastoma.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NCAM-1/CD56 Antibody (NBP2-34518)(1)

Publication using NBP2-34518

• Mundy-Bosse BL, Weigel C, Wu YZ Et al. Identification and Targeting of the Developmental Blockade in Extranodal Natural Killer/T-cell Lymphoma Blood Cancer Discov 2022-03-05 [PMID: 35247900] (IF/IHC)
  
  Details:
  Citation using the Azide and BSA Free version of this antibody.

Review for NCAM-1/CD56 Antibody (NBP2-34518) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Aida Meghraoui Imaging Mass Cytometry Human 10/05/2020

Summary

• Application: Other
• Sample Tested: FFPE
• Species: Human
• Lot: 4684-2PABX200611

Bioinformatics

• Gene Symbol: NCAM1