NAPRT1 Antibody (CL0665) - BSA Free

Western Blot: NAPRT1 Antibody (CL0665) [NBP2-52954] - Lane 1: Marker [kDa], Lane 2: Human liver tissue lysate

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human testis shows moderate cytoplasmic positivity in seminiferous tubules.

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human breast cancer shows positivity in tumor cells.

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human colorectal cancer shows strong immunoreactivity in tumor cells.

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human kidney shows strong cytoplasmic immunoreactivity in renal tubules.

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human placenta shows strong immunoreactivity in the trophoblast.

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human skeletal muscle shows absence of immunoreactivity (negative control).

Immunohistochemistry-Paraffin: NAPRT1 Antibody (CL0665) [NBP2-52954] - Staining of human stomach shows strong positivity in glandular cells.

• Reactivity: Hu, Mu, Rt
• Applications: WB, IHC
• Clone: CL0665
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free

NAPRT1 Antibody (CL0665) - BSA Free Summary

• Description: Novus Biologicals Mouse NAPRT1 Antibody (CL0665) - BSA Free (NBP2-52954) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed using a recombinant protein derived from Q6XQN6, with the exact immunogen sequence remaining proprietary.
• Predicted Species: Mouse (97%), Rat (96%). Backed by our 100% Guarantee.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPRT
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:200 - 1:500
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.
• Control Peptide:

  NAPRT1 Recombinant Protein Antigen (NBP2-52954PEP)

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Protein A purified

Alternate Names for NAPRT1 Antibody (CL0665) - BSA Free

• EC 2.4.2.11
• FHA-HIT-interacting protein
• FHIP
• NAPRTase
• nicotinate phosphoribosyltransferase domain containing 1
• Nicotinate phosphoribosyltransferase domain-containing protein 1
• nicotinate phosphoribosyltransferase
• nicotinic acid phosphoribosyltransferase
• PP3856

Background

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPRT