NAPRT1 Antibody (CL0366) - Azide and BSA Free

Lane 1: Marker [kDa]Lane 2: Human liver tissue lysate

Analysis in human duodenum and cerebral cortex tissues using NBP3-43973 antibody. Corresponding NAPRT1 RNA-seq data are presented for the same tissues.

Staining of human liver shows moderate cytoplasmic positivity in hepatocytes.

Staining of human cerebral cortex shows no positivity in neurons as expected.

Staining of human kidney shows strong cytoplasmic positivity in cells in tubules.

Staining of human duodenum shows strong cytoplasmic positivity in glandular cells.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: CL0366
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: LYOPH

NAPRT1 Antibody (CL0366) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse NAPRT1 Antibody (CL0366) - Azide and BSA Free (NBP2-52924) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated using a recombinant protein sequence of Q6XQN6, with the exact immunogen sequence remaining proprietary.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAPRT1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1:200 - 1:500
  • Western Blot 1 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A purified
• Reconstitution Instructions: Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Alternate Names for NAPRT1 Antibody (CL0366) - Azide and BSA Free

• EC 2.4.2.11
• FHA-HIT-interacting protein
• FHIP
• NAPRTase
• nicotinate phosphoribosyltransferase domain containing 1
• Nicotinate phosphoribosyltransferase domain-containing protein 1
• nicotinate phosphoribosyltransferase
• nicotinic acid phosphoribosyltransferase
• PP3856

Background

Nicotinic acid (NA; niacin) is converted by nicotinic acid phosphoribosyltransferase (NAPRT; EC 2.4.2.11) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD), which serves as a coenzyme in cellular redox reactions and is an essential component of a variety of processes in cellular metabolism including response to stress (Hara et al., 2007).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAPRT1