NAP1L4 Antibody

Western Blot: NAP1L4 Antibody [NBP2-36543] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NAP-2 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Immunoprecipitation: NAP1L4 Antibody [NBP2-36543] - Detection of human NAP-2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-NAP-2 antibody NBP2-36543 used for IP at 6 ug per reaction. NAP-2 was also immunoprecipitated by rabbit anti-NAP-2 antibody NBP2-36542. For blotting immunoprecipitated NAP-2, NBP2-36543 was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

NAP1L4 Antibody Summary

• Immunogen: The immunogen recognized by the antibody maps to a region between residue 325 to 375 of human Nucleosome Assembly Protein 2 using the numbering given in entry NP_005960.1.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAP1L4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 ug/mg
  • Western Blot 1:2000 - 1:10000
• Application Notes: Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
  Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for NAP1L4 Antibody

• hNAP2
• MGC4565
• NAP-2
• NAP2L
• NAP2NAP1L4b
• nucleosome assembly protein 1-like 4
• nucleosome assembly protein 1-like 4b
• Nucleosome assembly protein 2

Background

NAP1L4 (Nucleosome Assembly Protein 1-Like 4) is thought to have involvement as a histone chaperone. Due to its location near the imprinted gene domain of 11p15.5, NAP1L4 has been studied in relation to Wilms tumor, Beckwith-Wiedemann syndrome, rhabdomyosarcoma and several forms of cancer including breast, lung and ovarian cancer. NAP1L4 is known to have interactions with HIST1H4A, HIST2H4A, HIST1H4AB, HIST1H4C and HIST1H4D.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAP1L4
• Uniprot:
  • NP_005960.1(Human)