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NAA25 Antibody

Images

 
Western Blot: NAA25 Antibody [NBP1-88391] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Human cell line RT-4Lane 3: Human cell line U-251MG sp
Immunocytochemistry/ Immunofluorescence: NAA25 Antibody [NBP1-88391] - Staining of human cell line U-2 OS shows positivity in cytoplasm & the Golgi apparatus. Antibody staining is shown in green.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ICC/IF
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NAA25 Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: TGKRFIEKDIQYPFLGPVPTRMGGFFNSGCSQCQISSFYLVNDIYELDTSGLEDTMEIQERIENSFKSLLDQLKDVFSKCKGDLL
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NAA25
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
Application Notes
Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Control Peptide
NAA25 Protein (NBP1-88391PEP)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for NAA25 Antibody

  • C12orf30DKFZp667K2112
  • chromosome 12 open reading frame 30
  • FLJ13089
  • MDM20mitochondrial distribution and morphology 20
  • Mitochondrial distribution and morphology protein 20
  • N(alpha)-acetyltransferase 25, NatB auxiliary subunit
  • N-alpha-acetyltransferase 25, NatB auxiliary subunit
  • NAP1
  • NatB complex subunit MDM20
  • N-terminal acetyltransferase B complex subunit MDM20
  • N-terminal acetyltransferase B complex subunit NAA25
  • p120

Background

NAA25, also known as MDM20, is a component of N-acetyltransferase complex B (NatB). Human NatB performs cotranslational N(alpha)-terminalacetylation of methionine residues when they are followed by asparagine (Starheim et al., 2008 (PubMed18570629)).(supplied by OMIM)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Bioinformatics

Gene Symbol NAA25