NCKAP1 Antibody

Western Blot: NCKAP1 Antibody [NBP2-19491] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491] - NCKAP1 Antibody detects NCKAP1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody diluted at 1:250. Red: NF-H, stained by NF-H antibody diluted at 1:500. Blue: Fluoroshield with DAPI.

Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491] - Human lung Papillary adenocarcinoma, using NCKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

• Reactivity: Hu, Mu, Rt, RM, Xp, Ze
• Applications: WB, IHC, IHC-Fr, IHC-P
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NCKAP1 Antibody Summary

• Immunogen: Recombinant protein encompassing a sequence within the N-terminus region of human NCKAP1. The exact sequence is proprietary.
• Localization: Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity); Cell projection; lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side (By similarity)
• Predicted Species: Rat (97%), Xenopus (94%), Zebrafish (92%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NCKAP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
• Theoretical MW: 129 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol (pH7)
• Preservative: 0.025% Proclin 300
• Purity: Immunogen affinity purified

Alternate Names for NCKAP1 Antibody

• FLJ11291
• HEM2p125Nap1
• KIAA0587
• Membrane-associated protein HEM-2
• MGC8981
• NAP 1
• NAP125
• NAP1Nap1
• NCK-associated protein 1

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NCKAP1 Antibody (NBP2-19491)(1)

Publication using NBP2-19491

• Denes C, Newsome T, Miranda-Saksena M et al. A putative WAVE regulatory complex (WRC) interacting receptor sequence (WIRS) in the cytoplasmic tail of HSV-1 gE does not function in WRC recruitment or neuronal transport Access Microbiol 2021-06-21 [PMID: 34151161]

Bioinformatics

• Gene Symbol: NCKAP1
• Uniprot:
  • Q9Y2A7(Human)