NCKAP1 Antibody

Images

 
Western Blot: NCKAP1 Antibody [NBP2-19491] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with NCKAP1 antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated ...read more
Immunohistochemistry-Frozen: NCKAP1 Antibody [NBP2-19491] - NCKAP1 Antibody detects NCKAP1 protein by immunohistochemical analysis. Sample: Frozen-sectioned mouse cerebellum. Green: NCKAP1 stained by NCKAP1 antibody ...read more
Immunohistochemistry-Paraffin: NCKAP1 Antibody [NBP2-19491] - Human lung Papillary adenocarcinoma, using NCKAP1 antibody at 1:250 dilution. Antigen Retrieval: Trilogy™ (EDTA based, pH 8.0) buffer, 15min.

Product Details

Summary
Reactivity Hu, Mu, Rt, RM, Xp, ZeSpecies Glossary
Applications WB, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

NCKAP1 Antibody Summary

Immunogen
Recombinant protein encompassing a sequence within the N-terminus region of human NCKAP1. The exact sequence is proprietary.
Localization
Cell membrane; Single-pass membrane protein; Cytoplasmic side (By similarity); Cell projection; lamellipodium membrane; Single-pass membrane protein; Cytoplasmic side (By similarity)
Predicted Species
Rat (97%), Zebrafish (92%), Xenopus (94%), Rhesus Macaque (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
NCKAP1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Frozen 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Western Blot 1:500-1:3000
Theoretical MW
129 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using NBP2-19491.

Reactivity Notes

Xenopus laevis (94%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS, 20% Glycerol (pH7)
Preservative
0.025% Proclin 300
Purity
Immunogen affinity purified

Alternate Names for NCKAP1 Antibody

  • FLJ11291
  • HEM2p125Nap1
  • KIAA0587
  • Membrane-associated protein HEM-2
  • MGC8981
  • NAP 1
  • NAP125
  • NAP1Nap1
  • NCK-associated protein 1

Background

Is part of lamellipodial complex that controls Rac-dependent actin remodeling.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-19491
Species: Hu, Mu, Rt, RM, Xp, Ze
Applications: WB, IHC
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for NCKAP1 Antibody (NBP2-19491)(1)

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol NCKAP1
Uniprot