Nanog Antibody (5A10)

Images

 
Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat ...read more
Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium ...read more
Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clone
5A10
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Nanog Antibody (5A10) Summary

Immunogen
Recombinant human Nanog (1-154aa) purified from E. coli (NP_079141).
Marker
Embryonic Stem Cell Marker
Specificity
Anti-human Nanog mAb, clone 5A10, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human Nanog protein.
Isotype
IgG2a Kappa
Clonality
Monoclonal
Host
Mouse
Gene
NANOG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:1000
  • ELISA 1:100-1:2000
  • Flow Cytometry
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:50-1:100
Theoretical MW
35 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
NBP1-04320 in the following applications:

  • IHC
    2 publications
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.4) and 10% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Nanog Antibody (5A10)

  • FLJ12581
  • FLJ40451
  • hNanog
  • homeobox protein NANOG
  • Homeobox transcription factor Nanog
  • homeobox transcription factor Nanog-delta 48
  • Nanog homeobox
  • Nanog

Background

Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Nanog Antibody (NBP1-04320)(6)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(2)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 6 of 6.
Publications using NBP1-04320 Applications Species
Gong W, Sun B, Sun H et al. Nodal signaling activates the Smad2/3 pathway to regulate stem cell-like properties in breast cancer cells. Am J Cancer Res. Apr 12 2017 [PMID: 28401007] (WB, Human) WB Human
Koh SP, On N, Brasch HD et al. Embryonic Stem Cell-like Population in Dupuytren's Disease. Plast Reconstr Surg Glob Open. Nov 1 2016 [PMID: 27975007] (IHC, Human) IHC Human
Yu HH, Featherston T, Tan ST et al. Characterization of Cancer Stem Cells in Moderately Differentiated Buccal Mucosal Squamous Cell Carcinoma. Front Surg. Aug 17 2016 [PMID: 27532037] (IHC, Human) IHC Human
Kumar B, Yadav A, Lang JC et al. Suberoylanilide hydroxamic acid (SAHA) reverses chemoresistance in head and neck cancer cells by targeting cancer stem cells via the downregulation of nanog. Genes Cancer. 2015 Mar 01 [PMID: 26000099] (WB, Human)

Details:
Nanog antibody was used for WB application on lysates of human head and neck squamous cell carcinoma cell line CAL27 and UD-SCC-2 cell line as well as their cisplatin-resistant variants namely CAL27-CisR and UD-SCC-2-CisR respectively (Figure 3E-F). WB also performed on CAL27-CisR and UD-SCC-2-CisR cell that were subjected or not to HDAC1 / HDAC2 knocked down using siRNA (Figure 4A-B), and on CAL27-CisR and UD-SCC-2-CisR cells treated or not with Suberoylanilide Hydroxamic Acid /SAHA, an HDAC inhibitor (Figure 6C-D).
WB Human
Moretti A, Bellin M, Jung CB et al. Mouse and human induced pluripotent stem cells as a source for multipotent Isl1+ cardiovascular progenitors. FASEB J 2009 [PMID: 19850773]
Lin F, Prichard J, Bitting AK et al. Geisinger Immunohistochemical Antibodies and Staining Protocols Handbook of Practical Immunohistochemistry. 2015 (IHC-P, Human) IHC-P Human

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Product General Protocols

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Video Protocols

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Secondary Antibodies

 

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Blogs on Nanog.

KLF4 as a transcription factor in stem cell differentiation
Kru¨ppel-like factors (KLFs) are evolutionarily conserved zinc finger transcription factors that play a role in cell differentiation, proliferation, and pluripotency. KLF4 has specifically been tied to many diverse cellular processes, including sel...  Read full blog post.

Nanog is a Master Controller of ES cell Pluripotency
Nanog, a homeodomain (HD) transcription factor, plays a critical role in the maintenance of embryonic stem (ES) cell self-renewal. Transcription regulator involved in inner cell mass and ES cell proliferation and self-renewal. Imposes pluripotency on...  Read full blog post.

Sox2 and Oct4: Roles in Embryonic Stem Cell Pluripotency
Embryonic stem (ES) cells are cells derived from the inner cell mass of the blastocyst, an early-stage embryo. ES cells are distinguished from other cells due to their pluripotency, which is the ability to differentiate into any different type of cell...  Read full blog post.

An Unlikely Pairing: The SOX2 Antibody and Breast Cancer
SOX2 encodes a member of the SOX (SRY-related HMG-box) family of transcription factors, which play a vital role in embryonic development. SOX2 antibody research has identified Sox2 as a key transcription factor in pluripotent stem cells. We at Novus B...  Read full blog post.

Fluorescence Activated Cell Sorting Antibody Techniques
Recently, we at Novus Biologicals added several embryonic stem cell marker products to our antibody catalog, validated for use in fluorescent activated cell sorting (FACS) assays. They included Cripto1, PODXL, SSEA, OCT4, Nanog, SOX2, TRA-1, TERT and...  Read full blog post.

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Bioinformatics

Gene Symbol NANOG
Entrez
Uniprot