Western Blot: Nanog Antibody (5A10) [NBP1-04320] - The cell lysates of NIH3T3 (35ug) were resolved by SDS-PAGE, transferred to NC membrane and probed with anti-human Nanog (1:500). Proteins were visualized using a goat ...read more
Immunohistochemistry-Paraffin: Nanog Antibody (5A10) [NBP1-04320] - Human seminoma tissue were incubated with anti-human Nanog (1:50) for 2 hours at room temperature. Antigen retrieval was performed in 0.1M sodium ...read more
Flow Cytometry: Nanog Antibody (5A10) [NBP1-04320] - Flow cytometry analysis of Nanog in Hep3B cell line, staining at 2-5ug for 1x106cells. The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate.
35 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using NBP1-04320 in the following applications:
Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Additional Mouse on Mouse blocking steps may be required for IHC and ICC experiments. Please contact Technical Support for more information.
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.4) and 10% Glycerol
0.02% Sodium Azide
Protein G purified
Alternate Names for Nanog Antibody (5A10)
homeobox protein NANOG
Homeobox transcription factor Nanog
homeobox transcription factor Nanog-delta 48
Nanog is a multidomain homeobox transcription factor that functions to maintain the undifferentiated state of pluripotent stem cells. Nanog expression counteracts the differentiation-promoting signals induced by the extrinsic factors LIF, Stat3 and BMP. When Nanog expression is downregulated, cell differentiation can proceed. Proteins that regulate Nanog expression include transcription factors Oct4, SOX2, FoxD3, and Tcf3 and tumor suppressor p53.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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