Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Whole cell protein from NTERA-2 was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed ...read more
Immunocytochemistry/ Immunofluorescence: Nanog Antibody (1E6C4) [NBP1-47427] - Confocal immunofluorescence analysis of NTERA-2 cells (left) and HeLa cells (right) using anti-Nanog Mouse mAb (green). Red: Actin filaments ...read more
Western Blot: Nanog Antibody (1E6C4) [NBP1-47427] - Western blot analysis using anti-Nanog monoclonal antibody against NTERA-2 cell lysate (lane 2).
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Protein G purified
This Nanog (1E6C4) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence and ELISA. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
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Nanog is a divergent homeodomain protein that directs pluripotency and differentiation of undifferentiated embryonic stem cells. Nanog mRNA is present in pluripotent mouse and human cell lines, and absent from differentiated cells. Human Nanog protein shares 52% overall amino acid identity with the mouse protein and 85% identity in the homeodomain. Human Nanog maps to gene locus 12p13.31, whereas mouse Nanog maps to gene loci 6 F2. Murine embryonic Nanog expression is detected in the inner cell mass of the blastocyst.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.