NALP4 Antibody - BSA Free

Immunohistochemistry: NALP4 Antibody [NB100-56156] - Formalin-fixed, paraffin-embedded tissue cores from a human breast cancer microarray stained for Smac/Diablo using NB100-56156 at 1:2000. Hematoxylin-eosin counterstain. Differential Smac/DIABLO staining is seen between patient samples.

• Reactivity: Hu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NALP4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NALP4 Antibody - BSA Free (NB100-56156) is a polyclonal antibody validated for use in IHC and WB. Anti-NALP4 Antibody: Cited in 6 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: A synthetic peptide corresponding to amino acids 139-157 (FAPKETGKQPRTVIIQGPQ) of human NALP4/PAN2/PYPAF4 was used as immunogen; GenBank no. gi|17064172|gb|AAL35293.1|AF442488_1
• Specificity: NALP4
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NLRP4
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000-1:5000
  • Western Blot 1:1000-1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.02% Sodium Azide
• Purity: Unpurified

Alternate Names for NALP4 Antibody - BSA Free

• Cancer/testis antigen 58
• CLR19.5
• CT58PYRIN and NACHT-containing protein 2
• FLJ32126
• NACHT, leucine rich repeat and PYD containing 4
• NACHT, LRR and PYD domains-containing protein 4
• NALP4NACHT, LRR and PYD containing protein 4
• NLR family, pyrin domain containing 4
• nucleotide-binding oligomerization domain, leucine rich repeat and pyrin domaincontaining 4
• PAN2Ribonuclease inhibitor 2
• PYPAF4PAAD and NACHT-containing protein 2
• RNH2PYRIN-containing APAF1-like protein 4

Background

NALPs are cytoplasmic proteins that form a subfamily within the larger CATERPILLER protein family. Most short NALPs, such as NALP4, have an N-terminal pyrin (MEFV; MIM 608107) domain (PYD), followed by a NACHT domain, a NACHT-associated domain (NAD), and a C-terminal leucine-rich repeat (LRR) region. The long NALP, NALP1 (MIM 606636), also has a C-terminal extension containing a function to find domain (FIIND) and a caspase recruitment domain (CARD). NALPs are implicated in the activation of proinflammatory caspases (e.g., CASP1; MIM 147678) via their involvement in multiprotein complexes called inflammasomes (Tschopp et al., 2003).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for NALP4 Antibody (NB100-56156)(6)

Publications using NB100-56156

• Xiao M, Yang J, Dong M et Al. NLRP4 renders pancreatic cancer resistant to olaparib through promotion of the DNA damage response and ROS-induced autophagy Cell Death Dis 2024-08-26 [PMID: 39187531]
• Wu YL, Pan LH, Yi ZJ et al. c-Myb Dominates TBK1-Mediated Endotoxin Tolerance in Kupffer Cells by Negatively Regulating DTX4 Journal of immunology research 2023-03-31 [PMID: 37032653] (WB, Human)
• Ruscitti P, Berardicurti O, Di Cola I et al. The hyper-expression of NLRP4 characterises the occurrence of macrophage activation syndrome assessing STING pathway in adult onset Still’s disease Clinical and Experimental Immunology 2022-02-02 [PMID: 35467709]
• Yu J, Lin Y, Xiong X et al. Detection of Exosomal PD-L1 RNA in Saliva of Patients With Periodontitis Front Genet. 2019-03-13 [PMID: 30923536] (WB, Human)
• Yamamoto Mayuko, Kondo Eisaku, Takeuchi Makoto et al. miR-155, a Modulator of FOXO3a Protein Expression, Is Underexpressed and Cannot Be Upregulated by Stimulation of HOZOT, a Line of Multifunctional Treg. PLoS One. 2011-01-01 [PMID: 21304824] (WB, Human)
• Jounai N, Kobiyama K, Shiina M et al. NLRP4 negatively regulates autophagic processes through an association with beclin1. J Immunol. 2011-02-01 [PMID: 21209283] (WB)
  
  Details:
  NALP4/PAN2/PYPAF4.1. WB: Endogenous Beclin1 protein was immunoprecipitated from lysed HeLa cells then subject to WB with the NLRP4 (IMG-5743) antibody, Fig 1C.

Bioinformatics

• Gene Symbol: NLRP4
• Entrez:
  • 147945(Human)
  • 243880(Mouse)