NAIP Antibody (541609) [Unconjugated]

Western blot shows lysates of HEK293 human embryonic kidney cell line either mock transfected or transfected with human NAIP and human brain (cerebellum) tissue. PVDF Membrane was probed with 1 µg/mL of Human NAIP Monoclonal Antibody (Catalog # MAB829) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for NAIP at approximately 160 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

NAIP was detected in immersion fixed paraffin-embedded sections of human brain (cerebellum) using Human NAIP Monoclonal Antibody (Catalog # MAB829) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to Purkinje neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Hu
• Applications: WB, IHC
• Clone: 541609
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

NAIP Antibody (541609) [Unconjugated] Summary

• Immunogen: E.coli-derived recombinant human NAIP
  Asn923-Val1148
  Accession # Q13075
• Specificity: Detects human NAIP in Western blots. In direct ELISAs, no cross-reactivity with recombinant mouse (rm) NAIP or rmNAIP2 is observed.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: NAIP
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL
  • Immunohistochemistry 8-25 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for NAIP Antibody (541609) [Unconjugated]

• baculoviral IAP repeat-containing 1
• BIRC1
• BIRC1baculoviral IAP repeat-containing protein 1
• FLJ18088
• FLJ42520
• FLJ58811
• NAIP
• Neuronal apoptosis inhibitory protein
• NLR family, apoptosis inhibitory protein
• NLR family, BIR domain containing 1
• NLRB1
• nucleotide-binding oligomerization domain, leucine rich repeat and BIR domaincontaining 1
• psi neuronal apoptosis inhibitory protein
• psiNAIP

Background

Neuronal apoptosis inhibitory protein (NAIP; also Baculoviral IAP repeat-containing protein 1) is a 160 kDa member of the inhibitor of apoptosis family of proteins (also known as BIRC proteins). Human NAIP is 1403 amino acids (aa) in length.  It contains three distinct regions: an N-terminal cluster of three baculoviral inhibitory repeat (BIR) domains, a central nucleotide binding oligomerization domain (NOD), and a C-terminal leucine-rich repeat (LRR) domain.  Human NAIP shares 68% aa identity with mouse NAIP.  NAIP is expressed in motor neurons, but not in sensory neurons.  It is also expressed in the liver, placenta and to a lesser extent in the spinal cord.  NAIP prevents motor neuron apoptosis, and defects in NAIP have been found in individuals with spinal muscular atrophy.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAIP
• Uniprot:
  • Q13075()