NAGS Antibody

Western Blot: NAGS Antibody [NBP2-93763] - Analysis of extracts of various cell lines, using NAGS at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

NAGS Antibody Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 295-534 of human NAGS (NP_694551.1). NTGGLRDSSHKVLSNVNLPADLDLVCNAEWVSTKERQQMRLIVDVLSRLPHHSSAVITAASTLLTELFSNKGSGTLFKNAERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYVSEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSDGSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFHKPASDPGS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAGS
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500-1:1000
• Theoretical MW: 58 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for NAGS Antibody

• AGAS
• Amino-acid acetyltransferase
• ARGA
• EC 2.3.1.1
• MGC133025
• N-acetylglutamate synthase
• N-acetylglutamate synthase, mitochondrial
• NAT7

Background

NAGS (N-acetylglutamate synthase) is involved in the regulation of ureagenesis since it produces NAG, which is a cofactor of CPSI. NAGS is known to have interactions with TMEM39B, ASS1, ADSSL1, SNRPD3, and RPS16. NAGS has been studied in relation to several diseases and disorders including hepatitis, Urea Cycle Disorder, propionic academia, hyperargininemia, Celiac disease and metabolic disorders.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAGS