NAA10 Antibody - BSA Free

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Mouse lung using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Western Blot: NAA10 Antibody [NBP3-38359] - Western blot analysis of various lysates using NAA10 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Human stomach using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunocytochemistry/ Immunofluorescence: NAA10 Antibody [NBP3-38359] - Immunofluorescence analysis of U2OS cells using NAA10 Rabbit pAb. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Rat lung using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry: NAA10 Antibody [NBP3-38359] - Immunohistochemistry analysis of paraffin-embedded Human breast cancer using NAA10 Rabbit pAb at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NAA10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NAA10 Antibody - BSA Free (NBP3-38359) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 161-235 of human NAA10 (NP_003482.1).
  
  Sequence:
  HLELKEKGRHVVLGAIENKVESKGNSPPSSGEACREEKGLAAEDSGGDSKDLSEVSETTESTDVKDSSEASDSAS
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAA10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 26 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NAA10 Antibody - BSA Free

• ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
• ARD1 homolog, N-acetyltransferase (S. cerevisiae)
• ARD1
• ARD1AARD1 homolog, N-acetyltransferase
• EC 2.3.1.-
• EC 2.3.1.88
• MGC71248
• N(alpha)-acetyltransferase 10, NatA catalytic subunit
• N-acetyltransferase ARD1, human homolog of
• N-acetyltransferase
• N-alpha-acetyltransferase 10, NatA catalytic subunit
• N-terminal acetyltransferase complex ARD1 subunit homolog A

Background

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAA10