TRIM23 Antibody

Images

 
Western Blot: TRIM23 Antibody [NB100-78620] - Sample(30 ug of whole cell lysate)A:HeLa S3(GTX14654)10% SDS PAGE, antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: TRIM23 Antibody [NB100-78620] - HCT116 cells mock (left) and treated with 30 uM Cisplatin for 24 hrs (right) were fixed in 4% paraformaldehyde at RT for 15 min. Green: TRIM23 ...read more
Immunohistochemistry-Paraffin: TRIM23 Antibody [NB100-78620] - Paraffin-embedded gastric CA, using antibody at 1:100 dilution.
Immunocytochemistry/ Immunofluorescence: TRIM23 Antibody [NB100-78620] - Paraformaldehyde-fixed HeLa, using TRIM23 antibody (Green) at 1:500 dilution.
Immunocytochemistry/ Immunofluorescence: TRIM23 Antibody [NB100-78620] - Confocal immunofluorescence analysis (Olympus FV10i) of paraformaldehyde-fixed HeLa, using TRIM23 antibody (green) at 1:500 dilution. ...read more

Product Details

Summary
Reactivity Hu, Mu, MkSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

TRIM23 Antibody Summary

Immunogen
Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human TRIM23. The exact sequence is proprietary.
Localization
Cell membrane
Predicted Species
Monkey (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
TRIM23
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:3000
  • Immunocytochemistry/Immunofluorescence 1:100-1:1000
  • Immunohistochemistry 1:100-1:1000
  • Immunohistochemistry-Paraffin 1:100-1:1000
Theoretical MW
64 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NB100-78620 in the following applications:

Reactivity Notes

Expected cross reactivity based on sequence homology: Rhesus Monkey (100%).

Packaging, Storage & Formulations

Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.0), 1.0% BSA and 20% Glycerol
Preservative
0.01% Thimerosal
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for TRIM23 Antibody

  • ADP-ribosylation factor domain protein 1, 64kDa
  • ADP-ribosylation factor domain-containing protein 1
  • ARD1GTP-binding protein ARD-1
  • ARF domain protein 1
  • ARFD1
  • E3 ubiquitin-protein ligase TRIM23
  • EC 6.3.2.-
  • RNF46RING finger protein 46
  • tripartite motif containing 23
  • tripartite motif protein TRIM23
  • tripartite motif-containing 23
  • Tripartite motif-containing protein 23

Background

The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. This protein is also a member of the ADP ribosylation factor family of guanine nucleotide-binding family of proteins. Its carboxy terminus contains an ADP-ribosylation factor domain and a guanine nucleotide binding site, while the amino terminus contains a GTPase activating protein domain which acts on the guanine nucleotide binding site. The protein localizes to lysosomes and the Golgi apparatus. It plays a role in the formation of intracellular transport vesicles, their movement from one compartment to another, and phopholipase D activation. Three alternatively spliced transcript variants for this gene have been described.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for TRIM23 Antibody (NB100-78620)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: ICC/IF.


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ICC/IF
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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol TRIM23
Entrez