NAA10 Antibody - BSA Free

Western Blot: NAA10 Antibody [NBP2-33934] - Lane 1: Marker [kDa] 230, 130, 95, 72, 56, 36, 28, 17, 11. Lane 2: Human cell line RT-4. Lane 3: Human cell line U-251MG sp. Lane 4: Human plasma (IgG/HSA depleted). Lane 5: Human liver tissue. Lane 6: Human tonsil tissue

Immunocytochemistry/Immunofluorescence: NAA10 Antibody [NBP2-33934] - Staining of human cell line U-2 OS shows localization to cytosol. Antibody staining is shown in green.

Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-33934] - Staining of human fallopian tube shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-33934] - Staining of human testis shows weak to moderate cytoplasmic positivity in cells in seminiferous ducts.

Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-33934] - Staining of human duodenum shows moderate to strong cytoplasmic positivity in glandular cells.

Immunohistochemistry-Paraffin: NAA10 Antibody [NBP2-33934] - Staining of human endometrium shows weak to moderate cytoplasmic positivity in glandular cells.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

NAA10 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit NAA10 Antibody - BSA Free (NBP2-33934) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was developed against a recombinant protein corresponding to amino acids: AIENKVESKGNSPPSSGEACREEKGLAAEDSGGDSKDLSEVSETTESTDVKDSSEASDSAS
• Predicted Species: Mouse (90%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: NAA10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  NAA10 Protein (NBP2-33934PEP)

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Rat (89%)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for NAA10 Antibody - BSA Free

• ARD1 homolog A, N-acetyltransferase (S. cerevisiae)
• ARD1 homolog, N-acetyltransferase (S. cerevisiae)
• ARD1
• ARD1AARD1 homolog, N-acetyltransferase
• EC 2.3.1.-
• EC 2.3.1.88
• MGC71248
• N(alpha)-acetyltransferase 10, NatA catalytic subunit
• N-acetyltransferase ARD1, human homolog of
• N-acetyltransferase
• N-alpha-acetyltransferase 10, NatA catalytic subunit
• N-terminal acetyltransferase complex ARD1 subunit homolog A

Background

N-alpha-acetylation is one of the most common protein modifications that occurs during protein synthesis and involves the transfer of an acetyl group from acetyl-coenzyme A to the protein alpha-amino group. ARD1A, together with NATH (NARG1; MIM 608000), is part of a major N-alpha-acetyltransferase complex responsible for alpha-acetylation of proteins and peptides (Sanchez-Puig and Fersht, 2006 [PubMed 16823041]).[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: NAA10
• Uniprot:
  • P41227(Human)