Myosin Heavy Chain 1 Antibody (MY32)

Immunohistochemistry-Paraffin: Myosin Heavy Chain 1 Antibody (MY32) [NBP1-22811] - Formalin fixed paraffin embedded human skeletal muscle stained with Myosin Heavy Chain 1 antibody (NBP1-22811).

• Reactivity: Hu, Rb
• Applications: Simple Western, IHC
• Clone: MY32
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Myosin Heavy Chain 1 Antibody (MY32) Summary

• Description: This product has a prediluted size available. If a 6mL or 7mL pre-diluted size is purchased this product can be used Neat (without further dilution). Use recommended dilution for the concentrated vials only.
• Immunogen: Rabbit muscle myosin.
• Localization: Cytoplasmic
• Specificity: This antibody recognizes the heavy chain of human skeletal myosin. It does not stain cardiac or smooth muscle myosin. Rather, it stains the fast twitch (type II) isomyosin molecule.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYH1
• Purity: Ascites

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin
  • Simple Western
• Application Notes: IHC-P: recommended pretreatment of citrate buffer, pH 6.0. Recommended incubation time of 30 min at RT.
  See Simple Western Antibody Database for Simple Western validation: separated by Size

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: Ascites
• Preservative: 0.05% Sodium Azide
• Purity: Ascites

Alternate Names for Myosin Heavy Chain 1 Antibody (MY32)

• DFNA4
• FLJ13881
• FLJ43092
• KIAA2034DKFZp667A1311
• MHC16
• MYH1
• MYH14 variant protein
• MYH17
• MyHC-2x
• MyHC-IIx/d
• Myosin Heavy Chain 1
• Myosin heavy chain 14
• Myosin heavy chain, non-muscle IIc
• myosin
• myosin, heavy chain 14
• myosin, heavy chain 14, non-muscle
• myosin, heavy polypeptide 14
• myosin-14
• NMHC II-C
• NMHC-II-C
• Non-muscle myosin heavy chain IIc
• nonmuscle myosin heavy chain II-C

Background

Myosin II is the so called "classical" myosin, being the first type of myosin to be discovered. Myosin II forms bipolar filaments that interact with actin filaments to produce contraction. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. The myosin II molecule is composed of two heavy chains and two sets of light chains. The light chains are the Essential Light Chains (ELC) and Regulatory Light Chains (RLC). Overall activity and polymerization can be modulated by phosphorylation on the light chains and the C terminus of the heavy chains respectively.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myosin Heavy Chain 1 Antibody (NBP1-22811)(1)

Publication using NBP1-22811

• Sieck D, Kobak S, Larson E et al. Histamine is a molecular transducer of adaptation to endurance exercise training in humans Journal of applied physiology (Bethesda, Md. : 1985) 2025-06-01 [PMID: 40372922]

Bioinformatics

• Gene Symbol: MYH1