MYH7 Antibody (940906) [Unconjugated]

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MYH7 was detected in immersion fixed paraffin-embedded sections of human heart using Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) at 1 µg/mL overnight at 4 °C. Tissue was stained using the ...read more
Western blot shows lysates of human skeletal muscle tissue and human heart (atrium) tissue. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human MYH7 Monoclonal Antibody (Catalog # MAB9096) followed by ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC
Clone
940906
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

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Catalog# & Formulation Size Price

MYH7 Antibody (940906) [Unconjugated] Summary

Immunogen
Human MYH7 synthetic peptide
Accession # P12883
Specificity
Detects human MYH7 in direct ELISAs and Western blots.
Source
N/A
Isotype
IgG2b
Clonality
Monoclonal
Host
Mouse
Gene
MYH7
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1-25 ug/mL
  • Western Blot 0.5 ug/mL

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for MYH7 Antibody (940906) [Unconjugated]

  • cardiac muscle beta isoform
  • CMD1S
  • CMD1SMGC138376
  • CMH1
  • DKFZp451F047
  • MGC138378
  • MPD1
  • MYH7
  • MYHCB
  • MyHC-beta
  • myhc-slow
  • myopathy, distal 1
  • myosin heavy chain (AA 1-96)
  • myosin, heavy chain 7, cardiac muscle, beta
  • myosin, heavy polypeptide 7, cardiac muscle, beta
  • Myosin-7
  • rhabdomyosarcoma antigen MU-RMS-40.7A
  • SPMD
  • SPMM

Background

MYH7 encodes the beta myosin heavy chain (MHC-beta ) which is a component of cardiac muscle myosin mainly expressed in the ventricle of fetal heart and represents the minority myosin in the adult heart. This is the 'slow form' of cardiac myosin as opposed to the 'fast form' (MYH6, aka MHC-alpha ) expressed more predominantely in the atria of the fetal heart and is the predominant myosin in the adult heart. The two isoforms of cardiac MHC alpha  and  beta  display 93% homology but have significantly different enzymatic properties, with  alpha  having 150-300% the contractile velocity and 60-70% actin attachment time as that of beta . Several mutations in MYH7 have been associated with inherited cardiomyopathies paraspinal and proximal muscle atrophy. MYH7 is a 223 kDa protein composed of 1935 amino acids.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol MYH7
Uniprot