Myogenin Antibody (SPM144) [PerCP]

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Product Details

Summary
Reactivity Hu, Mu, Rt, Po, FeSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
SPM144
Clonality
Monoclonal
Host
Mouse

Myogenin Antibody (SPM144) [PerCP] Summary

Immunogen
Human myogenin recombinant protein
Localization
Nuclear
Marker
Skeletal Muscle Marker
Specificity
Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Anti-myogenin labels the nuclei of myoblasts in developing muscle tissue, and is expressed in tumor cell nuclei of rhabdomyosarcoma and some leiomyosarcomas. Positive nuclear staining may occur in Wilms tumor.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity
Protein A purified
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Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Conjugate
PerCP
Format
PerCP (A=490, E=675)
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Protein A purified

Applications/Dilutions

Dilutions
  • Western Blot
  • Flow Cytometry
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry-Frozen
  • Immunohistochemistry-Paraffin
  • Immunoprecipitation
Application Notes
Immunohistochemistry (Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Alternate Names for Myogenin Antibody (SPM144) [PerCP]

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myogenin Antibody (NBP2-34740PCP) (0)

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Secondary Antibodies

 

Isotype Controls

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Blogs on Myogenin

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Contact Information

Product PDFs

Bioinformatics

Gene Symbol MYOG
Entrez

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