Myogenin Antibody (5FD) - BSA Free

Images

 
Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line ...read more
Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Human myocytes using NB100-56703 at 2.5 ug/mL. Hematoxylin-eosin counterstain.
Immunohistochemistry-Frozen: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/mL.

Product Details

Summary
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    • Catalog Number
      NB100-56510
    • Availability
      Product Discontinued

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Myogenin Antibody (5FD) - BSA Free Summary

Immunogen
A recombinant protein corresponding to amino acids 30-224 of rat myogenin was used as immunogen (NP_002470).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/ Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:20 - 1:1000
  • Immunohistochemistry-Frozen 10 - 20 ug/mL
  • Immunohistochemistry-Paraffin 20 - 40 ug/mL
  • Western Blot 2 ug/mL
Reviewed Applications
Read 1 Review rated 4
using
NB100-56510 in the following applications:

Publications
Read Publications using
NB100-56510 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Myogenin Antibody (5FD) - BSA Free

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Background

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myogenin Antibody (NB100-56510)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 4 applications: FLOW, ICC/IF, IHC-P, WB.


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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-56510 Applications Species
Chenchen Li, Haigang Cao, Yingchun Ren, Jinrui Jia, Gongshe Yang, Jianjun Jin, Xin'e Shi Eicosapentaenoic acid-mediated activation of PGAM2 regulates skeletal muscle growth and development via the PI3K/AKT pathway. International journal of biological macromolecules 2024-04-17 [PMID: 38641281]
Nakazawa K, Shaw T, Song YK et al. Piperacetazine directly binds to the PAX3::FOXO1 fusion protein and inhibits its transcriptional activity Cancer research communications 2023-09-21 [PMID: 37732905] (WB, Human) WB Human
Cao, H;Du, T;Li, C;Wu, L;Liu, J;Guo, Y;Li, X;Yang, G;Jin, J;Shi, X; MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1 BMC genomics 2023-07-24 [PMID: 37488537]
Zhao T, Zhao R, Yi X et al. METTL3 promotes proliferation and myogenic differentiation through m6A RNA methylation/YTHDF1/2 signaling axis in myoblasts Life sciences 2022-03-26 [PMID: 35351467] (WB, Mouse) WB Mouse
Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 2021-09-14 [PMID: 34519272]
Flavie Ouali BE, Wang HV Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro Biochemistry and biophysics reports 2021-07-01 [PMID: 34041371] (WB, Mouse) WB Mouse
Peng Y, Xu M, Dou M et al. MicroRNA-129-5p Inhibited C2C12 Myogenesis and Repressed Slow Fiber Gene Expression in vitro American journal of physiology. Cell physiology 2021-04-07 [PMID: 33826407]
Cai R, Zhang Q, Wang Y et al. Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis The Journal of biological chemistry 2021-02-03 [PMID: 33548229] (WB, Mouse) WB Mouse
Liu S, Sun Y, Zhao R et al. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition Food Funct. 2020-11-16 [PMID: 33289736]
Osaki T, Uzel SGM, Kamm RD On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease Nat Protoc. [PMID: 31932771] (FLOW, Human) FLOW Human
Show All 15 Publications.

Review for Myogenin Antibody (NB100-56510) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NB100-56510:
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Images Ratings Applications Species Date Details
Western Blot Myogenin NB100-56510
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4
reviewed by:
Francesca Pacifici
WB Mouse 11/20/2017
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Summary

ApplicationWestern Blot
Sample TestedC2C12 mouse myoblast cell line
SpeciesMouse
LotAB111111A-2

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Recent Reviews

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Francesca Pacifici
11/20/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MYOG
Entrez
Uniprot