Myogenin Antibody (5FD) - BSA Free

Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.

Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Human myocytes using NB100-56703 at 2.5 ug/mL. Hematoxylin-eosin counterstain.

Immunohistochemistry-Frozen: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC
• Clone: 5FD
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

Myogenin Antibody (5FD) - BSA Free Summary

• Immunogen: A recombinant protein corresponding to amino acids 30-224 of rat myogenin was used as immunogen (NP_002470).
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 5 ug/mL
  • Immunohistochemistry 1:20 - 1:1000
  • Immunohistochemistry-Frozen 10 - 20 ug/mL
  • Immunohistochemistry-Paraffin 20 - 40 ug/mL
  • Western Blot 2 ug/mL

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Myogenin Antibody (5FD) - BSA Free

• BHLHC3
• bHLHc3Myogenic factor-4; myogenin
• Class C basic helix-loop-helix protein 3
• MYF4
• myf-4
• MYF4myogenin
• MYOG
• Myogenic factor 4
• myogenin (myogenic factor 4)
• Myogenin

Background

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myogenin Antibody (NB100-56510)(12)

Publications using NB100-56510

• Cao, H;Du, T;Li, C;Wu, L;Liu, J;Guo, Y;Li, X;Yang, G;Jin, J;Shi, X; MicroRNA-668-3p inhibits myoblast proliferation and differentiation by targeting Appl1 BMC genomics 2023-07-24 [PMID: 37488537] (B/N, WB)
• Zhao T, Zhao R, Yi X et al. METTL3 promotes proliferation and myogenic differentiation through m6A RNA methylation/YTHDF1/2 signaling axis in myoblasts Life sciences 2022-03-26 [PMID: 35351467] (WB, Mouse)
• Hammers DW, Hart CC, Matheny MK et al. Filopodia powered by class x myosin promote fusion of mammalian myoblasts eLife 2021-09-14 [PMID: 34519272] (B/N)
• Flavie Ouali BE, Wang HV Beta-agonist drugs modulate the proliferation and differentiation of skeletal muscle cells in vitro Biochemistry and biophysics reports 2021-07-01 [PMID: 34041371] (WB, Mouse)
• Peng Y, Xu M, Dou M et al. MicroRNA-129-5p Inhibited C2C12 Myogenesis and Repressed Slow Fiber Gene Expression in vitro American journal of physiology. Cell physiology 2021-04-07 [PMID: 33826407]
• Cai R, Zhang Q, Wang Y et al. Lnc-ORA interacts with microRNA-532-3p and IGF2BP2 to inhibit skeletal muscle myogenesis The Journal of biological chemistry 2021-02-03 [PMID: 33548229] (WB, Mouse)
• Liu S, Sun Y, Zhao R et al. Isoleucine increases muscle mass through promoting myogenesis and intramyocellular fat deposition Food Funct. 2020-11-16 [PMID: 33289736]
• Osaki T, Uzel SGM, Kamm RD On-chip 3D neuromuscular model for drug screening and precision medicine in neuromuscular disease Nat Protoc. [PMID: 31932771] (FLOW, Human)
• McCoy CR, Stadelman BS, Brumaghim JL et al. Arsenic and Its Methylated Metabolites Inhibit the Differentiation of Neural Plate Border Specifier Cells Chem. Res. Toxicol. 2015-06-10 [PMID: 26024302] (IHC-P, Mouse)
  
  Details:
  SOX10 antibody (clone 1E6) was used for IHC-P staining of embryoid bodies which were generated / differentiated from mouse P19 mouse embryonal carcinoma cell line using the hanging drop method (Wang and Yang 2008, J Visualized Exp. 17, 825). The staining assay implicated antigen rertieval via citric acid buffer pH 6 using microwave, 1 hour blocking with 1× PBS, 5%BSA, and 0.05% Tween-20, use of primary at 1:200 dilution with 4C ON incubation, detection with Alexa Fluor conjugated secondary antibody (Figure 2, Figure 4).
• Wu M, Yang G, Chen Y et al. CEP2 Attenuates Myoblast Differentiation But Does Not Affect Proliferation. Int. J. Biol. Sci. 2015-01-01 [PMID: 25552934] (ICC/IF, Human)
• Long Xiaochun, Bell Robert D, Gerthoffer William T et al. Myocardin is sufficient for a smooth muscle-like contractile phenotype. Arterioscler Thromb Vasc Biol. 2008-08-01 [PMID: 18451334]
• Dias P, Chen B, Dilday B, Palmer H, Hosoi H, Singh S, Wu C, Li X, Thompson J, Parham D, Qualman S, Houghton P. Strong immunostaining for myogenin in rhabdomyosarcoma is significantly associated with tumors of the alveolar subclass. Am J Pathol;156(2):399-408. 2000-02-01 [PMID: 10666368]

Review for Myogenin Antibody (NB100-56510) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Francesca Pacifici WB Mouse 11/20/2017

Summary

• Application: Western Blot
• Sample Tested: C2C12 mouse myoblast cell line
• Species: Mouse
• Lot: AB111111A-2

Bioinformatics

• Gene Symbol: MYOG
• Entrez:
  • 4656(Human)
  • 17928(Mouse)
  • 29148(Rat)
• Uniprot:
  • P15173()