Myogenin Antibody (5FD)

Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line (Rh30). Negative cell lines were PFSK-1, EB2, SK-N-SH, and SJSA-1.

Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Staining of Myogenin in formalin-fixed, paraffin-embedded human myocytes using NB100-56703 at 2.5 ug/ml. Hematoxylin-eosin counterstain.

Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in a frozen tissue section of human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/ml.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P
• Clone: 5FD
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 1.0 mg/ml

Myogenin Antibody (5FD) Summary

• Immunogen: A recombinant protein corresponding to amino acids 30-224 of rat myogenin was used as immunogen (NP_002470).
• Specificity: The 5FD clone has been highly published; users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kDa band should be observed. The can be used for gel shift assays.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MYOG
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 20-40 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Protein G purified

Alternate Names for Myogenin Antibody (5FD)

• BHLHC3
• bHLHc3Myogenic factor-4; myogenin
• Class C basic helix-loop-helix protein 3
• MYF4
• myf-4
• MYF4myogenin
• MYOG
• Myogenic factor 4
• myogenin (myogenic factor 4)
• Myogenin

Background

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myogenin Antibody (NB100-56510)(5)

Publications using NB100-56510

• McCoy C. Arsenic Targets Neural Plate Border Specifier Cells In P19 Cells MS Thesis. 2015 Jan 01 (IHC-P, Mouse)
  
  Details:
  Myogenin antibody used for IHC-P on the embryoid bodies differentiated from P19 mouse embryonal carcinoma cell line - 10% neutral buffered formalin fixation, 5um paraffin sections, microwave based citric acid buffer pH 6 antigen retrieval, blocking with 1XPBS - 5%BSA - 0.05% Tween-20, primary antibody used at 1:200 dilution with ON 4C incubation, detection with IF conjugated secondaries.
• McCoy CR, Stadelman BS, Brumaghim JL et al. Arsenic and Its Methylated Metabolites Inhibit the Differentiation of Neural Plate Border Specifier Cells Chem. Res. Toxicol. 2015 Jun 10 [PMID: 26024302] (IHC-P, Mouse)
  
  Details:
  Myogenin antibody (clone 5FD) was used for IHC-P staining of embryoid bodies which were generated / differentiated from mouse P19 mouse embryonal carcinoma cell line using the hanging drop method (Wang and Yang 2008, J Visualized Exp. 17, 825). The staining assay implicated antigen rertieval via citric acid buffer pH 6 using microwave, 1 hour blocking with 1× PBS, 5%BSA, and 0.05% Tween-20, use of primary at 1:200 dilution with 4C ON incubation, detection with Alexa Fluor conjugated secondary antibody (Figure 1, Figure 4).
• Wu M, Yang G, Chen Y et al. CEP2 Attenuates Myoblast Differentiation But Does Not Affect Proliferation. Int. J. Biol. Sci. 2015 Jan 01 [PMID: 25552934] (ICC/IF, Human)
• Long Xiaochun, Bell Robert D, Gerthoffer William T et al. Myocardin is sufficient for a smooth muscle-like contractile phenotype. Arterioscler Thromb Vasc Biol. 2008 Aug [PMID: 18451334]
• Dias P, Chen B, Dilday B, Palmer H, Hosoi H, Singh S, Wu C, Li X, Thompson J, Parham D, Qualman S, Houghton P. Strong immunostaining for myogenin in rhabdomyosarcoma is significantly associated with tumors of the alveolar subclass. Am J Pathol;156(2):399-408. 2000 Feb. [PMID: 10666368]

Review for Myogenin Antibody (NB100-56510) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Francesca Pacifici WB Mouse 11/20/2017

Summary

• Application: Western Blot
• Sample Tested: C2C12 mouse myoblast cell line
• Species: Mouse
• Lot: AB111111A-2

Bioinformatics

• Gene Symbol: MYOG
• Entrez:
  • 4656(Human)
  • 17928(Mouse)
  • 29148(Rat)
• Uniprot:
  • P15173()