Myogenin Antibody (5FD)

Images

 
Western Blot: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin expression in various small-round cell tumor lines. The antibody strongly reacted with a band at ~34 kDa in alveolar rhobdomyosarcoma line ...read more
Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Staining of Myogenin in formalin-fixed, paraffin-embedded human myocytes using NB100-56703 at 2.5 ug/ml. Hematoxylin-eosin counterstain.
Immunohistochemistry: Myogenin Antibody (5FD) [NB100-56510] - Analysis of Myogenin in a frozen tissue section of human alveolar rhabdomyosarcoma using NB100-56510 at 5 ug/ml.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-Fr, IHC-P
Clone
5FD
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Myogenin Antibody (5FD) Summary

Immunogen
A recombinant protein corresponding to amino acids 30-224 of rat myogenin was used as immunogen (NP_002470).
Specificity
The 5FD clone has been highly published; users are encouraged to consult the scientific literature for additional application information. In Rh-30, a 34 kDa band should be observed. The can be used for gel shift assays.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
MYOG
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 5 ug/ml
  • Immunohistochemistry 1:20-1:1000
  • Immunohistochemistry-Frozen 10-20 ug/ml
  • Immunohistochemistry-Paraffin 20-40 ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NB100-56510 in the following applications:

Publications
Read Publications using
NB100-56510 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.05% Sodium Azide
Concentration
1.0 mg/ml
Purity
Protein G purified

Alternate Names for Myogenin Antibody (5FD)

  • BHLHC3
  • bHLHc3Myogenic factor-4; myogenin
  • Class C basic helix-loop-helix protein 3
  • MYF4
  • myf-4
  • MYF4myogenin
  • MYOG
  • Myogenic factor 4
  • myogenin (myogenic factor 4)
  • Myogenin

Background

Myogenin is a member of the MyoD family of myogenic basic helix-loop-helix (bHLH) transcription factors that also includes MyoD, Myf-5, and MRF4 (also known as herculinor Myf-6). MyoD family members are expressed exclusively in skeletal muscle and play a key role in activating myogenesis by binding to enhancer sequences of muscle-specific genes. The regulatory domain of MyoD is approximately 70 amino acids in length and includes both a basic DNA binding motif and a bHLH dimerization motif. MyoD family members share about 80% amino acid homology in their bHLH motifs. Transfection of myogenin and other family members into a variety of non-muscle cells has been shown to either convert these cells to myogenic cells, or to transcriptionally activate a set of otherwise unexpressed muscle-specific genes. In addition to activating muscle specific genes, members of the MyoD family members activate their own transcription and transactivate the transcription of other MyoD family members. For example, transfection of myogenin into 10T1/2 cells or Swiss 3T3 cells results in the activation of theendogenous myogenin gene as well as transactivation of MyoD. Likewise, the transfection of MyoD into these cells results in the activation of MyoD as well as the transactivation of myogenin. Each member of the MyoD family has distinct roles in muscle development; myogenin plays a key role in muscle maturation. Myogenin migrates at a molecular weight of approx. 34 kDa by SDS-PAGE.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Myogenin Antibody (NB100-56510)(5)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 2 applications: ICC/IF, IHC-P.


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Showing Publications 1 - 5 of 5.
Publications using NB100-56510 Applications Species
McCoy CR, Stadelman BS, Brumaghim JL et al. Arsenic and Its Methylated Metabolites Inhibit the Differentiation of Neural Plate Border Specifier Cells Chem. Res. Toxicol. 2015 Jun 10 [PMID: 26024302] (IHC-P, Mouse)

Details:
Myogenin antibody (clone 5FD) was used for IHC-P staining of embryoid bodies which were generated / differentiated from mouse P19 mouse embryonal carcinoma cell line using the hanging drop method (Wang and Yang 2008, J Visualized Exp. 17, 825). The staining assay implicated antigen rertieval via citric acid buffer pH 6 using microwave, 1 hour blocking with 1× PBS, 5%BSA, and 0.05% Tween-20, use of primary at 1:200 dilution with 4C ON incubation, detection with Alexa Fluor conjugated secondary antibody (Figure 1, Figure 4).
IHC-P Mouse
Wu M, Yang G, Chen Y et al. CEP2 Attenuates Myoblast Differentiation But Does Not Affect Proliferation. Int. J. Biol. Sci. 2015 Jan 01 [PMID: 25552934] (ICC/IF, Human) ICC/IF Human
Long Xiaochun, Bell Robert D, Gerthoffer William T et al. Myocardin is sufficient for a smooth muscle-like contractile phenotype. Arterioscler Thromb Vasc Biol. 2008 Aug [PMID: 18451334]
Dias P, Chen B, Dilday B, Palmer H, Hosoi H, Singh S, Wu C, Li X, Thompson J, Parham D, Qualman S, Houghton P. Strong immunostaining for myogenin in rhabdomyosarcoma is significantly associated with tumors of the alveolar subclass. Am J Pathol;156(2):399-408. 2000 Feb. [PMID: 10666368]
McCoy C. Arsenic Targets Neural Plate Border Specifier Cells In P19 Cells MS Thesis. 2015 Jan 01 (IHC-P, Mouse)

Details:
Myogenin antibody used for IHC-P on the embryoid bodies differentiated from P19 mouse embryonal carcinoma cell line - 10% neutral buffered formalin fixation, 5um paraffin sections, microwave based citric acid buffer pH 6 antigen retrieval, blocking with 1XPBS - 5%BSA - 0.05% Tween-20, primary antibody used at 1:200 dilution with ON 4C incubation, detection with IF conjugated secondaries.
IHC-P Mouse

Review for Myogenin Antibody (NB100-56510) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: WB.

Reviews using NB100-56510:
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Images Ratings Applications Species Date Details
Western Blot Myogenin NB100-56510
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reviewed by:
Francesca Pacifici
WB Mouse 11/20/2017
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Summary

ApplicationWestern Blot
Sample TestedC2C12 mouse myoblast cell line
SpeciesMouse
LotAB111111A-2

Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for Myogenin Antibody (NB100-56510) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB100-56510AF405
Alexa Fluor 488 Labeled NB100-56510AF488
Alexa Fluor 647 Labeled NB100-56510AF647
Alexa Fluor 700 Labeled NB100-56510AF700
Biotin Labeled NB100-56510B
DyLight 350 Labeled NB100-56510UV
DyLight 405 Labeled NB100-56510V
DyLight 488 Labeled NB100-56510G
DyLight 550 Labeled NB100-56510R
DyLight 650 Labeled NB100-56510C
DyLight 680 Labeled NB100-56510FR
DyLight 755 Labeled NB100-56510IR
HRP Labeled NB100-56510H

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Recent Reviews

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5
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4
1
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2
0
1
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Francesca Pacifici
11/20/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MYOG
Entrez
Uniprot