Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Immunogen affinity purified
Reconstitute at 0.2 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Olfactomedin-1/Noelin-1 Antibody
AMYolfactomedin related ER localized protein
Neuronal olfactomedin-related ER localized protein
Olfactomedin-1 (neuronal olfactomedin-related endoplasmic reticulum-localized-1), also known as Olfactomedin-1 or pancortin, is a 75 kDa secreted extracellular matrix glycoprotein expressed in the brain (1-3). Alternate promoter usage and splicing creates four forms of Noelin (Noelin 1-4) that are combinations of a common central region with alternate N- and C-termini (3, 4). Noelin-1 is the longest of the four isoforms, encoding a 16 amino acid (aa) signal sequence and a 469 aa mature protein with a coiled-coil region for multimerization and an olfactomedin-like domain. The shortest isoforms, Noelin-3 and -4, are truncated within the coiled-coil region and lack the olfactomedin-like domain (4). In the species tested, these isoforms appear to oppose actions of the longer isoforms (5). All isoforms are capable of forming homo- or heterodimers through cysteines in their common central region (2). The C-terminus of Noelin-1 (mouse aa 482-485) contains a putative endoplasmic reticulum retention sequence that has not been included in the R&D Systems protein (6). Mouse Noelin-1 (aa 17-481) shares > 99%, 99%, 97%, 96% and 93% aa identity with corresponding regions of rat, human, opossum, chicken and Xenopus Noelin-1, respectively. In vivo expression patterns differ somewhat between species (1). In chick early development, Noelin-1 shows highest concentration in areas that produce neural crest cells and is produced by migrating neural crest cells (7). Overproduction of Noelin-1 prolongs neural crest cell formation (7). Noelin-1 is also expressed in mouse neural crest, but not frog (1-3). All three species express Noelin-1 later in differentiating neural tissues in the spinal cord, brain and cranial ganglia (1-3).
Moreno, T.A. & M. Bronner-Fraser (2002) Mech. Dev. 119:121.
Ando, K. et al. (2005) Neuroscience 133:947.
Nagano, T. et al. (1998) Brain Res. Mol. Brain Res. 53:13.
Danielson, P.E. et al. (1994) J. Neurosci. Res. 38:468.
Moreno, T.A. & M. Bronner-Fraser (2005) Dev. Biol. 288:434.
Moreno, T.A. & M. Bronner-Fraser (2001) Dev. Biol. 240:340.
Barembaum, M. et al. (2000) Nat. Cell Biol. 2:219.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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