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Frizzled-4 Antibody (145901)

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Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, CyTOF-ready, ICC/IF
Clone
145901
Clonality
Monoclonal
Host
Rat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price
Alexa Fluor 350
FAB194U-100UG
Alexa Fluor 405
FAB194V-100UG
Alexa Fluor 488
FAB194G-100UG
Alexa Fluor 532
FAB194X
Alexa Fluor 594
FAB194T-100UG
Alexa Fluor 647
FAB194R-100UG
Alexa Fluor 700
FAB194N-100UG
Alexa Fluor 750
FAB194S-100UG
Biotin
BAM194
DyLight 350
FAB194D
DyLight 405
FAB194E
DyLight 488
FAB194K
DyLight 550
FAB194L
DyLight 594
FAB194M
DyLight 650
FAB194W
DyLight 680
FAB194Y
DyLight 755
FAB194Z
FITC
FAB194F
HRP
FAB194H
Janelia Fluor 549
FAB194I
Janelia Fluor 646
FAB194J
PerCP
FAB194C
View Available Formulations
Catalog# & Formulation Size Price
Carrier Free
MAB194-100
Carrier Free
MAB194-SP
Carrier Free
FAB194V-100UG
Carrier Free
FAB194U-100UG
Carrier Free
FAB194T-100UG
Carrier Free
FAB194S-100UG
Carrier Free
FAB194R-100UG
Carrier Free
FAB194N-100UG
Carrier Free
FAB194G-100UG
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Frizzled-4 Antibody (145901) Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
Phe37-Glu180
Accession # Q61088
Specificity
Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
Source
N/A
Isotype
IgG2a
Clonality
Monoclonal
Host
Rat
Gene
FZD4
Purity
Protein A or G purified from hybridoma culture supernatant
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.
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Applications/Dilutions

Dilutions
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL
Reviewed Applications
Read 1 Review rated 3
using
MAB194 in the following applications:
Publications
Read Publications using
MAB194 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Protein A or G purified from hybridoma culture supernatant
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-4 Antibody (145901)

  • CD344 antigen
  • CD344
  • EVR1
  • exudative vitreoretinopathy 1
  • FEVR
  • frizzled (Drosophila) homolog 4
  • frizzled homolog 4 (Drosophila)
  • Frizzled4
  • Frizzled-4
  • Fz-4
  • FZD4
  • FZD4S
  • FzE4
  • GPCR
  • hFz4
  • MGC34390
  • WNT receptor frizzled-4

Background

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89% and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).
  1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
  2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
  3. Xu, Q. et al. (2004) Cell 116:883.
  4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
  5. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
  6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
  7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
  8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
  9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: Rhodopsin Antibody (B630) [NBP2-25160] - Western blot analyses of ABCA4 (above), GAPDH (middle), and RHO (below) protein levels in retinal tissue of dogs with the three different genotypes. Image collected and cropped by CiteAb from the following publication. 50 ug of protein samples were resolved by SDS-PAGE, transferred to nitrocellulose membrane, and immunoblotted with the following primary antibodies: ABCA4 (NBP1-30032, 1:1000) and Rhodopsin (NBP2-25160, 1:5000), followed by Anti-Mouse IgG horseradish peroxidase-conjugated secondary antibody (R&D Systems, HAF007, 1:5000. Image collected and cropped by CiteAb from the following publication (doi.org/10.1371/journal.pgen.1007873) licensed under a CC-BY licence.
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Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Staining in human adrenal gland and cerebral cortex tissues using anti-AKR1B1 antibody. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: AKR1B1 Antibody [NBP1-89146] - Analysis in human adrenal gland and liver tissues. Corresponding AKR1B1 RNA-seq data are presented for the same tissues.
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Applications: ICC/IF, IHC, IHC-P
Ferredoxin
Reductase Antibody
NBP2-38530
Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis in human cell lines A-549 and U-251MG using anti-FDXR antibody. Corresponding FDXR RNA-seq data are presented for the same cell lines. Loading control: anti-GAPDH.
Western Blot: Ferredoxin Reductase Antibody [NBP2-38530] - Analysis using Anti-FDXR antibody NBP2-38530 (A) shows similar pattern to independent antibody NBP2-38706 (B).
Immunohistochemistry-Paraffin: Ferredoxin Reductase Antibody [NBP2-38530] - Staining of human adrenal gland shows strong granular cytoplasmic positivity in glandular cells.
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB

Publications for Frizzled-4 Antibody (MAB194)(3)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: Flow Cytometry, IHC-P, Western Blot.


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Review for Frizzled-4 Antibody (MAB194) (1) 31

Average Rating: 3
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using MAB194:
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Images Ratings Applications Species Date Details
Western Blot Frizzled-4 MAB194
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reviewed by:
Verified Customer
 WB Mouse 06/28/2015
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Summary

ApplicationWestern Blot
Sample Testedmouse ear mesenchymal stem cell
SpeciesMouse

Blocking

Blocking Details5% milk

Primary Anitbody

Dilution Ratio1:1000

Secondary Antibody

Secondary DescriptionRat IgG, HRP-linked whole antibody (from goat)
Secondary Manufacturer Cat#Amersham NA935
Secondary Concentration1:3000

Details

Detection Notesmouse EMSCs were infected with lentiviruses that express either the scrambled shRNA or shRNA against Fzd4

Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for Frizzled-4 Antibody (MAB194) (0)

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Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 FAB194U
Alexa Fluor 405 FAB194V
Alexa Fluor 488 FAB194G
Alexa Fluor 532 FAB194X
Alexa Fluor 594 FAB194T
Alexa Fluor 647 FAB194R
Alexa Fluor 700 FAB194N
Alexa Fluor 750 FAB194S
Biotin BAM194
DyLight 350 FAB194D
DyLight 405 FAB194E
DyLight 488 FAB194K
DyLight 550 FAB194L
DyLight 594 FAB194M
DyLight 650 FAB194W
DyLight 680 FAB194Y
DyLight 755 FAB194Z
FITC FAB194F
HRP FAB194H
Janelia Fluor 549 FAB194I
Janelia Fluor 646 FAB194J
PerCP FAB194C
Learn about Custom Antibody Labeling

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Western Blot Frizzled-4 MAB194
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 Verified Customer
06/28/2015
Application: WB
Species: Mouse
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Bioinformatics

Gene Symbol FZD4
Uniprot