Frizzled-4 Antibody (145901)

• Reactivity: Hu, Mu
• Applications: WB, Flow, CyTOF-ready, ICC/IF
• Clone: 145901
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Unconjugated
• Concentration: LYOPH

Frizzled-4 Antibody (145901) Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-4
  Phe37-Glu180
  Accession # Q61088
• Specificity: Detects human and mouse Frizzled-4 in Western blots. In Western blots, no cross-reactivity with recombinant mouse (rm) Frizzled-1, -2, -3, -6, -7, -8, or recombinant human Frizzled-5 is observed. Detects Frizzled-4 by flow cytometry and immunohistochemistry on mouse differentiated embryoid bodies derived from the mouse embryonic cell line, D3.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: FZD4
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2.5 ug/10^6 cells
  • Immunocytochemistry 8-25 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: Protein A or G purified from hybridoma culture supernatant
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Frizzled-4 Antibody (145901)

• CD344 antigen
• CD344
• EVR1
• exudative vitreoretinopathy 1
• FEVR
• frizzled (Drosophila) homolog 4
• frizzled homolog 4 (Drosophila)
• Frizzled4
• Frizzled-4
• Fz-4
• FZD4
• FZD4S
• FzE4
• GPCR
• hFz4
• MGC34390
• WNT receptor frizzled-4

Background

Frizzled-4, designated CD344, is a 7-transmembrane glycoprotein of the Frizzled family within the G-protein coupled receptor superfamily (1, 2). Frizzled proteins function as receptors for Wnt proteins and can activate canonical Wnt/beta-catenin signaling as well as planar cell polarity and calcium flux pathways (1). Frizzled-4 is particularly important in angiogenic Wnt pathway signaling (1, 5). Frizzleds contain a divergent N-terminal signal peptide, a highly conserved ~120  amino acid (aa) cysteine-rich domain (CRD), a variable length linker region, seven transmembrane domains, and a variable-length C-terminal tail (1). The mouse Frizzled-4 cDNA encodes 537 aa with a 36 aa signal sequence and a 186 aa N-terminal extracellular sequence (4). The portion expressed includes aa 37-180, and shares 93%, 94%, 90%, 89% and 88% identity with human, rat, equine, bovine and canine Frizzled-4, respectively. This portion competes for Wnt binding with endogenous receptors. In humans, a 122 aa soluble form that diverges at aa 95 is proposed to be a positive regulator of Wnt signaling pathways (5). Frizzled-4 is unusual in binding a non-wnt ligand, Norrin, in addition to binding Wnt ligands (1, 3, 6). Norrin binds the Frizzled-4 CRD, activates Wnt signaling pathways and uses LRP5/6 as co-receptors (3, 6). Deletion of either Frizzled-4 or Norrin in mice results in a similar phenotype including malformation of vasculature in the retina, cerebellar degeneration, and loss of hair cells in the inner ear (1, 3, 7). In humans, blindness due to familial exudative vitreoretinopathy (FEVR) is associated with mutations producing loss of function of Frizzled-4 or Norrin, designated EVR1 and EVR2, respectively (1, 3, 8). Frizzled-4 expression has been found in many tissues, including mouse ovary, where it influences corpus luteum vasculogenesis and is necessary for fertility (4, 9).

1. Huang, H-C. and P.S. Klein (2004) Genome Biol. 5:234.
2. Parmalee, N.L. and J. Kitajewski (2008) Curr. Drug Targets 9:558.
3. Xu, Q. et al. (2004) Cell 116:883.
4. Wang, Y. et al. (1996) J. Biol. Chem. 271:4468.
5. Sagara, N. et al. (2001) Biochem. Biophys. Res. Commun. 282:750.
6. Smallwood, P.M. et al. (2007) J. Biol. Chem. 282:4057.
7. Wang, Y. et al. (2001) J. Neurosci. 21:4761.
8. Robitaille, J. et al. (2002) Nat. Genet. 32:326.
9. Hsieh, M. et al. (2005) Biol. Reprod. 73:1135.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Frizzled-4 Antibody (MAB194)(3)

Publications using MAB194

• W Liu, L Zhang, K Xuan, C Hu, L Li, Y Zhang, F Jin, Y Jin Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3? Complex in Hypophosphatasia Theranostics, 2018;8(20):5575-5592. 2018 [PMID: 30555565] (Western Blot, Human)
• Baksh D, Boland GM, Tuan RS Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. J. Cell. Biochem., 2007;101(5):1109-24. 2007 [PMID: 17546602] (Flow Cytometry, Human)
• Chen J, Park CS, Tang SJ Activity-dependent synaptic Wnt release regulates hippocampal long term potentiation. J. Biol. Chem., 2006;281(17):11910-6. 2006 [PMID: 16501258] (IHC-P, Mouse)

Review for Frizzled-4 Antibody (MAB194) (1)

Average Rating: 3

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: HIROYUKI MORI WB Mouse 06/28/2015

Summary

• Application: Western Blot
• Sample Tested: mouse ear mesenchymal stem cell
• Species: Mouse

Bioinformatics

• Gene Symbol: FZD4
• Uniprot:
  • Q61088()