MYH4 Antibody - BSA Free

Western Blot: MYH4 Antibody - BSA Free [NBP3-46981] - Human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with (MYH4 Antibody) at dilution of 1:500

Immunohistochemistry: MYH4 Antibody - BSA Free [NBP3-46981] - Immunohistochemistry of paraffin-embedded human skeletal muscle tissue slide using (MYH4 Antibody) at dilution of 1:50

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MYH4 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MYH4 Antibody - BSA Free (NBP3-46981) is a polyclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: myosin, heavy chain 4, skeletal muscle. (Uniprot# Q9Y623)
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MYH4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunohistochemistry 1:20-1:200
  • Western Blot 1:200-1:1000
• Theoretical MW: 230 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS, 50% Glycerol, pH (7.3)
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MYH4 Antibody - BSA Free

• MYH4
• MyHC-2b
• MyHC-IIb
• myosin heavy chain 4
• myosin heavy chain IIb
• myosin heavy chain, skeletal muscle, fetal
• myosin, heavy chain 4, skeletal muscle
• myosin, heavy polypeptide 4, skeletal muscle
• myosin-4

Background

MYH4, also known as Myosin-4, is a 1939 amino acid protein that is 223 kDa, predominantly found in myocytes mediates plus-ended movement along microfilaments and involved in muscle contraction through cyclic interactions with actin-rich thin filaments, creating a contractile force. It is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations. Current research is being performed on several diseases and disorders including congestive heart failure, yaws, myocarditis, hermaphroditism, liver disease, pharyngitis, and alcoholism. This protein is involved in cell adhesion integrin-mediated cell adhesion and migration, cell adhesion tight junctions, cytoskeleton remodeling regulation of actin cytoskeleton by Rho GTPases, immune response CCR3 signaling in eosinophils, development MAG-dependent inhibition of neurite outgrowth, RhoA, antioxidant action of vitamin-C, transendothelial migration of leukocytes, actin nucleation by ARP-WASP complex, epithelial adherens junctions, inhibitory action of lipoxins on neutrophil migration, membrane trafficking, translocation of GLUT4 to the plasma membrane, translocation of GLUT4 vesicle and docking at the plasma membrane, and Viral myocarditis pathways forming interactions with ESR2, NTHL1, S100A4, USP15, C12orf44, CNBP, ACTB, and over 30 other proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MYH4