MYH Antibody

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Western Blot: MYH Antibody [NB600-1032] - analysis of MYH in 20 ugs of HeLa cell lysate using this antibody.

Product Details

Summary
Reactivity Hu, Mu, Rt, BaSpecies Glossary
Applications WB, ICC/IF, IHC
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

MYH Antibody Summary

Immunogen
This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 531-546 of human MYH.
Localization
Nuclear
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MUTYH
Purity
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/ml
  • Immunocytochemistry/Immunofluorescence 18 ug/ml
  • Immunohistochemistry 1:10- 1:2000
Application Notes
Western Blot: Predicted molecular weight: 65.5 kDa. Immunocytochemistry/Immunofluorescence and Immunohistochemistry were reported in scientific literature.
Positive Control
HeLa Lysate (NB800-PC1)
Publications
Read Publications using
NB600-1032 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • WB
    4 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS containing 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Protein G purified

Alternate Names for MYH Antibody

  • CYP2C
  • EC 3.2.2
  • EC 3.2.2.-
  • hMYH
  • MGC4416
  • mutY (E. coli) homolog
  • mutY homolog (E. coli)
  • MutY homolog
  • MYHA/G-specific adenine DNA glycosylase

Background

MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MYH Antibody (NB600-1032)(8)

We have publications tested in 3 confirmed species: Mouse, Rat, Bacteria.

We have publications tested in 3 applications: ICC/IF, IHC, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
WB
(4)
All Applications
Filter By Species
Mouse
(3)
Rat
(2)
Bacteria
(1)
All Species
Showing Publications 1 - 8 of 8.
Publications using NB600-1032 Applications Species
Fang M, Ohman Strickland PA, Kang HG, Zarbl, H. Uncoupling genotoxic stress responses from circadian control increases susceptibility to mammary carcinogenesis. Oncotarget. 2017 May 16 [PMID: 28427145] (WB, Rat) WB Rat
Turco E, Ventura I, Minoprio A et al. Understanding the role of the Q338H MUTYH variant in oxidative damage repair. Nucleic Acids Res 2013 Mar 4 [PMID: 23460202] (WB, Mouse) WB Mouse
Zaika E, Wei J, Yin D et al. p73 protein regulates DNA damage repair. FASEB J. 2011 Sep 2. [PMID: 21891782]
D'Agostino VG, Minoprio A, Torreri P et al. Functional analysis of MUTYH mutated proteins associated with familial adenomatous polyposis DNA Repair (Amst) 2010 Jun 4 [PMID: 20418187] (WB, Bacteria) WB Bacteria
Molatore S, Russo MT, D'Agostino VG et al. MUTYH mutations associated with familial adenomatous polyposis: functional characterization by a mammalian cell-based assay. Hum Mutat 2010 Feb [PMID: 19953527] (WB, ICC/IF, Mouse) WB, ICC/IF Mouse
Bonde, P, Gao, D, Chen, L, Miyashita, T, Montgomery, E, Harmon, J W, Wei, C. Duodenal Reflux Leads to Down Regulation of DNA Mismatch Repair Pathway in an Animal Model of Esophageal Cancer,. 83, 433-440. 2007 [PMID: 17257966]
Gao, D et al. Oxidative DNA damage DNA repair enzyme expression inversely related in murine models of fatty liver disease. Am J Physiol: Gastrointestinal Liver Physiology 287:1070-1077. 2004 [PMID: 15231485]
Wang AL, Lukas TJ, Yuan M et al. Age-related increase in mitochondrial DNA damage and loss of DNA repair capacity in the neural retina. Neurobiol Aging. 2008 Dec 10. [PMID: 1908429] (IHC, Mouse, Rat) IHC Mouse, Rat

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MYH Antibody (NB600-1032) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MUTYH
Entrez
Uniprot