MYH Antibody

Western Blot: MYH Antibody [NB600-1032] - Analysis of MYH in 20 ugs of HeLa cell lysate.

• Reactivity: Hu, Mu, Rt, Ba
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MYH Antibody Summary

• Description: Novus Biologicals Rabbit MYH Antibody (NB600-1032) is a polyclonal antibody validated for use in IHC, WB and ICC/IF. Anti-MYH Antibody: Cited in 7 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: This antibody was generated by immunizing rabbits with a synthetic peptide corresponding to amino acids 531-546 of human MYH.
• Localization: Nuclear
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MUTYH
• Purity: Protein G purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 18 ug/ml. Use reported in scientific literature
  • Immunohistochemistry 1:10- 1:2000. Use reported in scientific literature
  • Western Blot 2 ug/ml
• Application Notes: Western Blot: Predicted molecular weight: 65.5 kDa.
• Control:

  HeLa Whole Cell Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS containing 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Protein G purified

Alternate Names for MYH Antibody

• EC 3.2.2
• EC 3.2.2.-
• EC 3.2.2.31
• hMYH
• MGC4416
• MutY homolog
• MUTYH
• Muyth
• MYH
• MYHA/G-specific adenine DNA glycosylase

Background

MYH is the eukaryotic homolog to the E. coli protein MutY. It was first identified in HeLa cells and nicks DNA replication errors, specifically A/G mismatches.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MYH Antibody (NB600-1032)(7)

Publications using NB600-1032

• Fang M, Ohman Strickland PA, Kang HG, Zarbl, H. Uncoupling genotoxic stress responses from circadian control increases susceptibility to mammary carcinogenesis. Oncotarget. 2017-05-16 [PMID: 28427145] (WB, Rat)
• Turco E, Ventura I, Minoprio A et al. Understanding the role of the Q338H MUTYH variant in oxidative damage repair. Nucleic Acids Res 2013-03-04 [PMID: 23460202] (WB, Mouse)
• Zaika E, Wei J, Yin D et al. p73 protein regulates DNA damage repair. FASEB J. 2011-09-02 [PMID: 21891782]
• D'Agostino VG, Minoprio A, Torreri P et al. Functional analysis of MUTYH mutated proteins associated with familial adenomatous polyposis DNA Repair (Amst) 2010-06-04 [PMID: 20418187] (WB, Bacteria)
• Molatore S, Russo MT, D'Agostino VG et al. MUTYH mutations associated with familial adenomatous polyposis: functional characterization by a mammalian cell-based assay. Hum Mutat 2010-02-01 [PMID: 19953527] (ICC/IF, WB, Mouse)
• Bonde, P, Gao, D, Chen, L, Miyashita, T, Montgomery, E, Harmon, J W, Wei, C. Duodenal Reflux Leads to Down Regulation of DNA Mismatch Repair Pathway in an Animal Model of Esophageal Cancer, . 83, 433-440. 2007-01-01 [PMID: 17257966]
• Gao, D et al. Oxidative DNA damage DNA repair enzyme expression inversely related in murine models of fatty liver disease. Am J Physiol: Gastrointestinal Liver Physiology 287:1070-1077. 2004-01-01 [PMID: 15231485]

Bioinformatics

• Gene Symbol: MUTYH
• Entrez:
  • 4595(Human)
• Uniprot:
  • NP_001121897(Human)