Myeloperoxidase/MPO Antibody

Product Discontinued

Myeloperoxidase/MPO Antibody Summary

• Immunogen: A synthetic peptide corresponding to a sequence mapping at the C-terminal of human MPO, identical to the related mouse and rat sequence.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 10.5-1 ug/ml
  • Immunohistochemistry-Paraffin 10.5-1 ug/ml
  • Western Blot 2 ug/ml

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, and 0.2mg Na2HPO4
• Preservative: 0.05mg Thimerosal, 0.05mg Sodium Azide
• Purity: Immunogen affinity purified
• Reconstitution Instructions: Please note the larger size(s) come in lyophilized form and the sample size will be in liquid formate. Reconstitute with deionized water.

Alternate Names for Myeloperoxidase/MPO Antibody

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Publications for Myeloperoxidase/MPO Antibody (NBP1-42591)(5)

Publications using NBP1-42591

• Xiong B, Li M, Xiang S, Han L. A1AR-mediated renal protection against ischemia/reperfusion injury is dependent on HSP27 induction. Int Urol Nephrol. 2018-02-26 [PMID: 29480441] (IF/IHC, Mouse)
• Ramanathan M Jr, London NR Jr, Tharakan A et al. Airborne Particulate Matter Induces Non-allergic Eosinophilic Sinonasal Inflammation in Mice. Am J Respir Cell Mol Biol 2017-02-28 [PMID: 28245149]
• Shogan BD, Belogortseva N, Luong PM et al. Collagen degradation and MMP9 activation by Enterococcus faecalis contribute to intestinal anastomotic leak. Sci Transl Med. 2015-05-06 [PMID: 25947163] (WB, Rat)
• Dong B, Zhou H, Han C et al. Ischemia/Reperfusion-Induced CHOP Expression Promotes Apoptosis and Impairs Renal Function Recovery: The Role of Acidosis and GPR4. PLoS OnE. 2014-10-25 [PMID: 25343248] (IHC-P, Mouse)
• Zhang S, Han C-H, Chen X-S et al. Transient Ureteral Obstruction Prevents against Kidney Ischemia/Reperfusion Injury via Hypoxia-Inducible Factor (HIF)-2[alpha] Activation. PLoS ONE 20127(1):e29876. [PMID: 22295069]

Bioinformatics

• Gene Symbol: MPO
• Entrez:
  • 4353(Human)
  • 17523(Mouse)
  • 303413(Rat)
• Uniprot:
  • P05164()