Myeloperoxidase/MPO Antibody (JM10-58)

Western Blot: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Western blot analysis of Myeloperoxidase/MPO on HL-60 cell lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.

Western Blot: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1,000 dilution.

Immunocytochemistry/Immunofluorescence: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/Immunofluorescence: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: Myeloperoxidase/MPO Antibody (JM10-58) [NBP2-66964] - Analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.

Product Discontinued

Myeloperoxidase/MPO Antibody (JM10-58) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human Myeloperoxidase/MPO aa 178-219 / 745. (SwissProt: P05164 Human)
• Localization: Lysosome.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MPO
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Western Blot 1:500-1:1000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Myeloperoxidase/MPO Antibody (JM10-58)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

FUNCTION: Part of the host defense system of polymorphonuclear leukocytes. It is responsible for microbicidal activity against a wide range of organisms. In the stimulated PMN, MPO catalyzes the production of hypohalous acids, primarily hypochlorous acid in physiologic situations, and other toxic intermediates that greatly enhance PMN microbicidal activity. MPO is an important marker for myeloid cells, from the promyelocyte stage and to the mature forms. CATALYTIC ACTIVITY: Donor + H2O2 = oxidized donor + 2 H2O. CATALYTIC ACTIVITY: Cl- + H2O2 = HOCl + 2 H2O. COFACTOR: Binds 1 calcium ion per heterodimer. COFACTOR: Binds 1 heme B (iron-protoporphyrin IX) group covalently per heterodimer. SUBUNIT: Tetramer of two light chains and two heavy chains. SUBCELLULAR LOCATION: Lysosome. ALTERNATIVE PRODUCTS: 3 named isoforms produced by alternative splicing. DISEASE: Defects in MPO are the cause of myeloperoxidase deficiency (MPD). MPD is an autosomal recessive defect that results in disseminated candidiasis. SIMILARITY: Belongs to the peroxidase family. XPO subfamily.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Myeloperoxidase/MPO Antibody (NBP2-66964)(1)

Publication using NBP2-66964

• Alhasan H, Terkawi MA, Matsumae G et al. Inhibitory role of Annexin A1 in pathological bone resorption and therapeutic implications in periprosthetic osteolysis Nature communications 2022-07-07 [PMID: 35798730] (IHC-P, Human)

Bioinformatics

• Gene Symbol: MPO