Myeloperoxidase/MPO Antibody (9B12G7)

Western Blot: Myeloperoxidase/MPO Antibody (9B12G7) [NBP1-47430] - Analysis using MPO mouse mAb against truncated Trx-MPO recombinant protein (1),truncated MBP-MPO (aa1-193) recombinant protein (2) and truncated MPO (aa165-745 )-hIgGFc transfected CHO-K1 cell lysate (3).

Product Discontinued

Myeloperoxidase/MPO Antibody (9B12G7) Summary

• Immunogen: Purified recombinant fragment of MPO(aa1-193) expressed in E. Coli.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MPO
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Western Blot 1:500 - 1:2000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for Myeloperoxidase/MPO Antibody (9B12G7)

• EC 1.11.1
• EC 1.11.1.7
• MPO
• Myeloperoxidase

Background

Human myeloperoxidase (MPO) is a dimeric protein composed of two heavy subunits (53 kDa) and two light subunits (15 kDa). Each MPO molecule contains two prosthetic porphyrins which play an important role in the catalytic cycle. Molecular weights for MPO isoforms from pools of normal human samples range from 114,000 to 140,000 daltons reflecting a heterogeneous mixture of isoforms when assayed under non-reducing conditions of SDS-PAGE. Often MPO from a single donor will yield a homogenous preparation reflecting a single isoform. The carbohydrate component of MPO, consisting of mannose, glucose and N-acetylglucosamine residues is 2.5%. MPO is inhibited by azide and other compounds. MPO is stored in primary granules of neutrophils and serves as a bactericidal agent in that MPO catalyzes the production of hypochlorous acid (HOCl), a powerful oxidant. HOCl is derived from chloride ion (Cl-) and hydrogen peroxide (H2O2). In a number of inflammatory situations, MPO is released into the extracellular matrix where its measurement can be used as an indication of neutrophil activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MPO
• Entrez:
  • 4353(Human)
• Uniprot:
  • P05164()