MuSK Antibody (10A4)

Immunocytochemistry/Immunofluorescence: MuSK Antibody (10A4) [NBP1-47441] - Human neurons stained with MuSK antibody. ICC/IFF image submitted by a verified customer review.

Immunohistochemistry-Paraffin: MuSK Antibody (10A4) [NBP1-47441] - Immunohistochemical analysis of paraffin-embedded human lung cancer (A), muscles (B) and breast cancer (C) using anti-MUSK antibody with DAB staining.

Immunocytochemistry/Immunofluorescence: MuSK Antibody (10A4) [NBP1-47441] - Confocal immunofluorescence analysis of HEK293 cells transfected with extracellular MUSK (aa24-209)-hIgGFc using anti-MUSK monoclonal antibody (green). Blue: DRAQ5 fluorescent DNA dye.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, IHC
• Clone: 10A4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

MuSK Antibody (10A4) Summary

• Immunogen: Purified recombinant extracellular fragment of human MUSK (aa24-209) fused with hIgGFc tag expressed in HEK293 cell line.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUSK
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • ELISA 1:10000
  • Immunocytochemistry/Immunofluorescence 1:200-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Paraffin 1:200-1000
• Theoretical MW: 97 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: Ascites
• Preservative: 0.03% Sodium Azide
• Purity: Unpurified

Alternate Names for MuSK Antibody (10A4)

• EC 2.7.10
• EC 2.7.10.1
• MGC126323
• MGC126324
• muscle, skeletal, receptor tyrosine kinase
• MuSK
• skeletal receptor tyrosine-protein kinase

Background

Intercellular communication is often mediated by receptors on the surface of one cell that recognize and are activated by specific protein ligands released by other cells. Members of one class of cell surface receptors, receptor tyrosine kinases (RTKs), are characterized by having a cytoplasmic domain containing intrinsic tyrosine kinase activity. This kinase activity is regulated by the binding of a cognate ligand to the extracellular portion of the receptor. DeChiara et al. (1996) [PubMed 8653786] noted that the RTKs, known to be expressed in cell type-specific fashions, play a role critical for the growth and differentiation of those cell types. For example, members of the neural-specific TRK family that recognize nerve growth factor are absolutely required for the survival and development of discrete neuronal subpopulations, and the receptor tyrosine kinases TIE1 (MIM 600222) and TIE2 (MIM 600221) play a critical role in the development of normal blood vessels.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MuSK Antibody (NBP1-47441) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Ashleigh Dean IF Human 08/12/2021

Summary

• Application: Immunofluorescence
• Sample Tested: neurons
• Species: Human
• Lot: 10A4

Bioinformatics

• Gene Symbol: MUSK