Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Unconjugated]

Western blot shows lysates of human heart tissue, mouse heart tissue, and rat heart tissue. PVDF membrane was probed with 0.2 µg/mL of Mouse Anti-Human Muscle Phospho-fructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 85 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western lane view shows lysates of human heart tissue and rat heart tissue, loaded at 0.5 mg/mL. A specific band was detected for Muscle Phosphofructokinase/PFKM at approximately 84 kDa (as indicated) using 2 µg/mL of Mouse Anti-Human/Mouse/Rat Muscle Phosphofructokinase/PFKM Monoclonal Antibody (Catalog # MAB7687). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

• Reactivity: Hu, Mu, Rt
• Applications: WB, Simple Western
• Clone: 842735
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: LYOPH

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human Muscle Phosphofructokinase/PFKM
  Asn674-Val780
  Accession # P08237
• Specificity: Detects human Muscle Phosphofructokinase/PFKM in direct ELISAs and Western blots. Detects mouse and rat Muscle Phosphofructokinase/PFKM in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: PFKM
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Simple Western 2 ug/mL
  • Western Blot 0.2 ug/mL
• Application Notes: In Simple Western only 10-15 uL of the recommended dilution is used per data point.

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Sterile PBS to a final concentration of 0.5 mg/mL.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Unconjugated]

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

PFKM (PhosphoFructoKinase, Muscle type; also PFK-A and Phosphohexokinase)) is a 85-86 kDa member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru-6-P, forming Fru-1,6-bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740 aa catalytic region (aa 16-755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71 aa upstream of the standard site, while another shows a deletion of aa 282-312. Proteolysis of PKFM apparently occurs, generating 45-47 kDa fragments. Over aa 674-780, human PFKM shares 98% aa sequence identity with mouse PFKM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (MAB7687)(4)

Publications using MAB7687

• Fiorillo, M;Ricci, E;Fava, M;Longobucco, C;Sotgia, F;Rizza, P;Lanzino, M;Bonofiglio, D;Conforti, FL;Catalano, S;Barone, I;Morelli, C;Aquila, S;Lisanti, MP;Sisci, D; FoxO3a Drives the Metabolic Reprogramming in Tamoxifen-Resistant Breast Cancer Cells Restoring Tamoxifen Sensitivity Cells 2023-12-06 [PMID: 38132097] (Western Blot, Human)
• L Li, L Li, W Li, T Chen, Bin Zou, L Zhao, H Wang, X Wang, L Xu, X Liu, D Wang, B Li, TW Mak, W Du, X Yang, P Jiang TAp73-induced phosphofructokinase-1 transcription promotes the Warburg effect and enhances cell proliferation Nat Commun, 2018-11-08;9(1):4683. 2018-11-08 [PMID: 30409970] (Western Blot, Human)
• Kayoko Yoshida, Chiyo K. Imamura, Kanako Hara, Mayumi Mochizuki, Yusuke Tanigawara Effect of everolimus on the glucose metabolic pathway in mouse skeletal muscle cells (C2C12) Metabolomics 8/1/2017 [PMID: 28781589]
• Ulrik Winning Iepsen, Gregers Druedal Wibe Munch, Mette Rugbjerg, Anders Rasmussen Rinnov, Morten Zacho, Stefan Peter Mortensen et al. Effect of endurance versus resistance training on quadriceps muscle dysfunction in COPD: a pilot study International Journal of Chronic Obstructive Pulmonary Disease 11/9/2016 [PMID: 27822028]

Bioinformatics

• Gene Symbol: PFKM
• Uniprot:
  • P08237(Human)