Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Alexa Fluor® 488]

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 842735
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor 488

Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant human Muscle Phosphofructokinase/PFKM
  Asn674-Val780
  Accession # P08237
• Specificity: Detects human Muscle Phosphofructokinase/PFKM in direct ELISAs and Western blots. Detects mouse and rat Muscle Phosphofructokinase/PFKM in Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Muscle Phosphofructokinase/PFKM/PFK-1 Antibody (842735) [Alexa Fluor® 488]

• 6-phosphofructokinase, muscle type
• EC 2.7.1,6-phosphofructo-1-kinase
• EC 2.7.1.11
• GSD7
• MGC8699
• Muscle Phosphofructokinase
• PFK1
• PFK-1
• PFKA
• PFK-A
• PFKM
• PFKX
• Phosphofructo-1-kinase isozyme A
• Phosphofructokinase 1
• phosphofructokinase, muscle
• phosphofructokinase, polypeptide X
• Phosphofructokinase-1
• Phosphofructokinase-M
• Phosphohexokinase

Background

PFKM (PhosphoFructoKinase, Muscle type; also PFK-A and Phosphohexokinase)) is a 85-86 kDa member of the phosphofructokinase family, two domain subfamily of enzymes. It is one of three functionally related enzymes, all expressed by distinct genes. While PFKM is expressed by virtually all cells, its name is based on the fact that skeletal muscle expresses only the PFKM isotype. PFK catalyzes the essentially irreversible phosphorylation of Fru-6-P, forming Fru-1,6-bisP. This determines the rate of cellular glycolysis. As a monomer, PFKM is unstable, and following synthesis, PFKM forms low activity homodimers. High activity comes with tetramerization/oligomerization. In all cases, multimerization is associated with PFKM interaction with various components of the cell cytoskeleton. Human PFKM is 780 amino acids (aa) in length. It contains a 740 aa catalytic region (aa 16-755) plus one utilized phosphorylation site at Thr2. There are at least two potential isoform variants. One contains an alternative start site 71 aa upstream of the standard site, while another shows a deletion of aa 282-312. Proteolysis of PKFM apparently occurs, generating 45-47 kDa fragments. Over aa 674-780, human PFKM shares 98% aa sequence identity with mouse PFKM.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • P08237(Human)