Musashi-1 Antibody (SJ201)

Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (SJ201) [NBP2-67418] - Staining Musashi 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: Musashi-1 Antibody (SJ201) [NBP2-67418] - Analysis of paraffin-embedded human pancreas tissue using anti-Musashi 1 antibody. Counter stained with hematoxylin.

Flow Cytometry: Musashi-1 Antibody (SJ201) [NBP2-67418] - Analysis of Musashi 1 was done on Hela cells. The cells were fixed, permeabilized and stained with the primary antibody (1/50) (red). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated Goat anti-Rabbit IgG Secondary antibody at 1/1000 dilution for 30 minutes.Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Immunocytochemistry/Immunofluorescence: Musashi-1 Antibody (SJ201) [NBP2-67418] - Staining Musashi 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Product Discontinued

Musashi-1 Antibody (SJ201) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within C-terminal human Musashi-1. (SwissProt: O43347 Human)
• Localization: Cytoplasm, Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MSI1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:50-1:100
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:50-1:200
  • Immunoprecipitation
  • Western Blot 1:1000
• Application Notes: IP: Use at an assay dependent concentration.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Musashi-1 Antibody (SJ201)

• MSI1
• Musashi (Drosophila) homolog 1
• musashi homolog 1 (Drosophila)
• Musashi1
• Musashi-1
• RNA-binding protein Musashi homolog 1

Background

Musashi1, a neural RNA-binding protein, plays an important role in regulating cell differentiation in precursor cells. Musashi-1 (Msi-1) has been shown to increase the accumulation of tau isoforms in intracellular inclusions in dementia. It may play a role in the pathogenesis of various neurodegenerative disorders. Musashi1 has also been detected in human tumor tissues such as gliomas and melanomas, suggesting its involvement in cancer development. Msi-1 also appears to play be an vital role in the development of several types of carcinoma such as human hepatoma, and may be a useful molecular marker for tumor detection.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MSI1