MUC5AC Antibody (45M1)

Immunohistochemistry: MUC5AC Antibody (45M1) [NBP2-15196] - Representative micrographs and high magnification insets illustrating the airway cell population of terminal bronchioles from conditional Nedd4-2-/- mice and littermate controls after 3 months of doxycycline induction with anti-Muc5ac and anti-CCSP antibodies (n = 4/group). Scale bars, 60 and 15 um (insets). Image collected and cropped by CiteAb from the following publication //www.nature.com/articles/s41467-020-15743-6) licensed under a CC-BY license.

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Analysis using the Azide and BSA Free version of NBP2-15196. Detection Human stomach.

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded Rat stomach stained with MUC5AC Monoclonal Antibody (45M1).

Immunohistochemistry-Paraffin: MUC5AC Antibody (45M1) [NBP2-15196] - Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with MUC5AC Monoclonal Antibody (45M1).

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Dot Blot: MUC5AC Antibody (45M1) [NBP2-15196] - Mucin MUC5AC expression & immunoreactivity in primary cells of the nasal cavity. (A) Transcription analysis (RT-PCR) of MUC5AC gene in olfactory epithelial primary cells (OEPC), respiratory epithelial primary cells (REPC), tumour cell line RPMI 2650 & the concha nasalis media (c.n. media). MUC5AC transcript signal was referenced to beta-actin transcript signal. The significance was calculated by comparison of the OEPC, REPC & RPMI 2650 data with the c.n. media transcription data using an unpaired t-test. * p < 0.05; n = 4; error bars represent mean ± SD. (B) Dot blot analysis of MUC5AC protein in lysates of OEPC, REPC, RPMI 2650 & c.n. media. All OEPC & REPC cultures shown in (A,B) were cultivated for 14 days in vitro in T flasks. (C) Immunoreactivity against MUC5AC in OEPC that were first cultured for 7 days in T flask with a minimum confluency of 70% then under ALI conditions additional 20 days. Apically secreted mucus was collected at the days indicated corresponding to a mucin production of 2 to 3 days. Statistical analysis: unpaired t-test, * p < 0.05 compared to the standard model RPMI 2650. Image collected & cropped by CiteAb from the following publication (//pubmed.ncbi.nlm.nih.gov/31374872), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

• Reactivity: Hu, Mu, Rt, Po, Ch, Fe, Ma, Pm, Rb, Bv(-)
• Applications: WB, DB, Flow, ICC/IF, IHC, mIF
• Clone: 45M1
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MUC5AC Antibody (45M1) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP2-32732)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
• Immunogen: M1 mucin preparation from the fluid of an ovarian mucinous cyst belonging to an O Le(a-b) patient
• Localization: Cytoplasmic
• Specificity: This monoclonal antibody recognizes the peptide core of gastric mucin M1 (recently identified as Mucin 5AC).Its epitope is located in the C-terminal cysteine rich part of the peptide core of MUC5AC. Its epitope is destroyed by beta-mercaptoethanol but not by periodate treatment. This mucin is present in primary ovarian mucinous cancer but usually absent in colorectal adenocarcinoma, thus showing an expression pattern opposite to MUC2. Together with a panel of antibodies, Anti-MUC5AC may be useful for differential identification of primary mucinous ovarian tumors from colon adenocarcinoma metastatic to the ovary. MUC5AC antibodies may also be useful for identification of intestinal metaplasia as well as in the identification of pancreatic carcinoma and pre-cancerous changes vs. normal pancreas.
• Isotype: IgG1 Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: MUC5AC
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Dot Blot
  • Flow (Intracellular)
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/ Immunofluorescence 1-2ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Frozen 0.5ug/ml
  • Immunohistochemistry-Paraffin 1-2 ug/ml
  • Multiplex Immunofluorescence
  • Western Blot
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
  Optimal dilution for a specific application should be determined.
  Use in IHC-P reported in scientific literature (PMID:34260916).

Reactivity Notes

Hedgehog. Does not react with Bovine.

Use in Mouse reported in scientific literature (PMID:34260916)

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 10 mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for MUC5AC Antibody (45M1)

• gastric mucin
• leB
• lewis B blood group antigen
• major airway glycoprotein
• MUC5
• mucin 5, subtypes A and C, tracheobronchial/gastric
• mucin 5AC, oligomeric mucus/gel-forming pseudogene
• mucin 5AC, oligomeric mucus/gel-forming
• mucin-5 subtype AC, tracheobronchial
• mucin-5AC
• TBM
• tracheobronchial mucin

Background

Mucin 5, subtype AC (MUC5AC) is a gel-forming secreted mucin that functions as a protective barrier on surface of epithelial tissues (1-2). MUC5AC is expressed primarily by goblet cells of the surface epithelium, including in the stomach, respiratory tract, gall bladder, endocervix, and endometrium (1-3). Human MUC5AC is located on chromosome 11p15.5, consisting of 5654 amino acids and has a N-terminus, tandem-repeat region (TRR), and a C-terminus, with a theoretical molecular weight of ~585 kDa (1-4). More specifically, the N-terminus contains four von Willebrand factor type D domains, where D1 also has a leucine zipper pattern (1). The TRR is further divided into nine CysD domains and four PTS-rich tandem repeats (1). Finally, the C-terminus has a D4-, B-, C-, and CK-domain (1). The TRR is a site of heavy O-glycosylation that functions in gel and polypeptide formation (1,3).

Given that MUC5AC is the primary mucin produced and secreted by cells lining airway, it is understandable that its expression is dysregulated in a number of respiratory diseases (1,3,5,6). For instance, MUC5AC is overexpressed in asthma and the protein is also more highly glycosylated, specifically fucosylated, in the disease state (1,3,7). Additionally, its overproduction is a key feature in chronic obstructive pulmonary disease (COPD) contributing to mucosal blockage (6). Multiple pathways are associated with overproduction of MUC5AC including NFkappaB, the primary pathway for production and secretion, and also the MAPK and STAT6 pathways (3). In addition to conventional, synthetic therapeutics agents, researchers are exploring natural MUC5AC inhibitors such as flavonoids, glycosides, and steroids to treat associated disorders (3).

References

1. Krishn, S. R., Ganguly, K., Kaur, S., & Batra, S. K. (2018). Ramifications of secreted mucin MUC5AC in malignant journey: a holistic view. Carcinogenesis. https://doi.org/10.1093/carcin/bgy019

2. Ballester, B., Milara, J., & Cortijo, J. (2019). Mucins as a New Frontier in Pulmonary Fibrosis. Journal of clinical medicine. https://doi.org/10.3390/jcm8091447

3. Samsuzzaman, M., Uddin, M. S., Shah, M. A., & Mathew, B. (2019). Natural inhibitors on airway mucin: Molecular insight into the therapeutic potential targeting MUC5AC expression and production. Life sciences. https://doi.org/10.1016/j.lfs.2019.05.041

4. Uniprot (P98088)

5. Li, J., & Ye, Z. (2020). The Potential Role and Regulatory Mechanisms of MUC5AC in Chronic Obstructive Pulmonary Disease. Molecules (Basel, Switzerland). https://doi.org/10.3390/molecules25194437

6. Bonser, L. R., & Erle, D. J. (2017). Airway Mucus and Asthma: The Role of MUC5AC and MUC5B. Journal of clinical medicine. https://doi.org/10.3390/jcm6120112

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MUC5AC Antibody (NBP2-15196)(28)

Publications using NBP2-15196

• Tao X, Li J, He J et al. Pinellia ternata (Thunb.) Breit. Attenuates the allergic airway inflammation of cold asthma via inhibiting the activation of TLR4-medicated NF-kB and NLRP3 signaling pathway Journal of ethnopharmacology 2023-05-31 [PMID: 37268256]
• Turner M, Cox K, Liu S et al. Specific Targeting and Labeling of Colonic Polyps in CPC-APC Mice with Mucin 5AC Fluorescent Antibodies: A Model for Detection of Early Colon Cancer Current Issues in Molecular Biology 2023-04-11 [PMID: 37185743] (IHC)
• Jung MA, Song HK, Jo K et al. Gleditsia sinensis Lam. aqueous extract attenuates nasal inflammation in allergic rhinitis by inhibiting MUC5AC production through suppression of the STAT3/STAT6 pathway Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie 2023-05-01 [PMID: 36921533] (IHC-P, Mouse)
  
  Details:
  Dilution used in IHC-P 1:1000
• Bu ML, Li MH, Feng M et al. Poly(I:C) Exacerbates Airway Goblet Cell Hyperplasia and Lung Inflammation in HDM-Exposed Balb/C Mice by YAP/FOXM1 Pathway International archives of allergy and immunology 2023-02-23 [PMID: 36822170]
• Koh K, Bonser L, Eckalbar W et al. Genomic characterization and therapeutic utilization of IL-13-responsive sequences in asthma Cell Genomics 2022-12-01 [PMID: 36777184] (FLOW, Human)
• Pyun BJ, Jo K, Lee JY et al. Caesalpinia sappan Linn. Ameliorates Allergic Nasal Inflammation by Upregulating the Keap1/Nrf2/HO-1 Pathway in an Allergic Rhinitis Mouse Model and Nasal Epithelial Cells Antioxidants (Basel, Switzerland) 2022-11-15 [PMID: 36421442] (IHC-P, Mouse)
  
  Details:
  Female BALB/c mouse nasal tissue. Dilution of 1:1000 was used
• Yusuke Ueda, Haruta Mogami, Yosuke Kawamura, Masahito Takakura, Asako Inohaya, Eriko Yasuda, Yu Matsuzaka, Yoshitsugu Chigusa, Shinji Ito, Masaki Mandai, Eiji Kondoh Cervical MUC5B and MUC5AC are Barriers to Ascending Pathogens During Pregnancy. The Journal of clinical endocrinology and metabolism 2022-11-24 [PMID: 36112402]
• Xu K, Liu XN, Zhang HB et al. Tear film instability is associated with weakened colocalization between occludin and MUC5AC in scopolamine-induced dry eye disease (DED) rats International ophthalmology 2023-02-01 [PMID: 35908134]
• Tao X, Liu H, Xia J et al. Processed product (Pinelliae Rhizoma Praeparatum) of Pinellia ternata (Thunb.) Breit. Alleviates the allergic airway inflammation of cold phlegm via regulation of PKC/EGFR/MAPK/PI3K-AKT signaling pathway Journal of ethnopharmacology 2022-09-15 [PMID: 35688257] (IF/IHC, Mouse)
• Jung O, Tung YT, Sim E et al. Development of human-derived, three-dimensional respiratory epithelial tissue constructs with perfusable microvasculature on a high-throughput microfluidics screening platform Biofabrication 2022-04-01 [PMID: 35166694] (Flow Cytometry)
• Chantal Cossette, Lisa A. Miller, Qiuji Ye, Shishir Chourey, Chintam Nagendra Reddy, Joshua Rokach, William S. Powell Targeting the OXE receptor with a selective antagonist inhibits allergen-induced pulmonary inflammation in non-human primates British journal of pharmacology 2022-01-07 [PMID: 34766334]
• Wohnhaas CT, Gindele JA, Kiechle T et al. Cigarette Smoke Specifically Affects Small Airway Epithelial Cell Populations and Triggers the Expansion of Inflammatory and Squamous Differentiation Associated Basal Cells Int J Mol Sci 2021-07-24 [PMID: 34299265] (FLOW)
  
  Details:
  This citation used the Alexa Fluor 405 format of this antibody.
• Hicks-Berthet J, Ning B, Federico A Et Al. Yap/Taz inhibit goblet cell fate to maintain lung epithelial homeostasis Cell reports 2021-07-13 [PMID: 34260916] (IHC-P)
• Zania Diabasana, Jeanne-Marie Perotin, Randa Belgacemi, Julien Ancel, Pauline Mulette, Claire Launois et al. Chr15q25 Genetic Variant rs16969968 Alters Cell Differentiation in Respiratory Epithelia International Journal of Molecular Sciences 2021-06-22 [PMID: 34206324]
• Ostedgaard LS, Price MP, Whitworth KM et al. Lack of airway submucosal glands impairs respiratory host defenses Elife 2020-10-07 [PMID: 33026343] (ICC, Porcine)
• Ancel J, Belgacemi R, Perotin JM et al. Sonic hedgehog signalling as a potential endobronchial biomarker in COPD Respir. Res. 2020-08-07 [PMID: 32767976] (ICC/IF, Human)
• Zarei K, Stroik MR, Gansemer ND et al. Early pathogenesis of cystic fibrosis gallbladder disease in a porcine model Lab. Invest. 2020-07-27 [PMID: 32719544] (IHC-P, Porcine)
• Duerr J, Leitz DHW, Szczygiel M et al. Conditional deletion of Nedd4-2 in lung epithelial cells causes progressive pulmonary fibrosis in adult mice Nat Commun 2020-04-24 [PMID: 32332792] (IF/IHC, Mouse)
• Belgacemi R, Luczka E, Ancel J Airway epithelial cell differentiation relies on deficient Hedgehog signalling in COPD EBioMedicine 2019-12-23 [PMID: 31877414] (IF/IHC, IHC-P, Human)
• Ladel S, Schlossbauer P et al. Improved In Vitro Model for Intranasal Mucosal Drug Delivery: Primary Olfactory and Respiratory Epithelial Cells Compared with the Permanent Nasal Cell Line RPMI 2650. Pharmaceutics 2019-01-08 [PMID: 31374872] (Dot Blot, Porcine)
• Rutledge EA, Parvez RK, Short KM et al. Morphogenesis of the kidney and lung requires branch-tip directed activity of the Adamts18 metalloprotease Dev. Biol. 2019-06-23 [PMID: 31242448] (IHC-F, Mouse)
• Kobayashi H, Yoshida S, Shirasawa N et al. Expression and localization of aromatase in human gastric mucosa : Immunohistochemical study using biopsy materials. Histochem. Cell Biol. 2018-08-28 [PMID: 30159783] (Human)
• Ostedgaard LS, Moninger TO, McMenimen JD et al. Gel-forming mucins form distinct morphologic structures in airways Proc. Natl. Acad. Sci. U.S.A. 2017-06-27 [PMID: 28607090] (ICC/IF, Porcine)
• Seidel V, Hoffmann R, Braun A et al. Distribution and morphology of Clara cells in common marmosets (Callithrix jacchus). J Med Primatol. 2013 Apr [PMID: 23473106]
• Seidel V, Hoffmann R, Braun A et al. Distribution and morphology of Clara cells in common marmosets (Callithrix jacchus). J Med Primatol. 2013-04-01 [PMID: 23473106]
• Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med 2010-11-01 [PMID: 20622026]
• Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med;182(10):1251-61. 2010 Nov 15. [PMID: 20622026]
• Meyerholz DK, Stoltz DA, Namati E et al. Loss of cystic fibrosis transmembrane conductance regulator function produces abnormalities in tracheal development in neonatal pigs and young children. Am J Respir Crit Care Med 2010-11-01 [PMID: 20622026]

Reviews for MUC5AC Antibody (NBP2-15196) (5)

Average Rating: 4.2

(Based on 5 reviews)

We have 5 reviews tested in 3 species: Human, Mouse, Rat.

reviewed by: Verified Customer IHC-P Rat 10/20/2021

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Stomach tissue
• Species: Rat
• Lot: 45M1

Comments

• Comments: 4% formaldehyde fixed paraffin sections

reviewed by: Verified Customer IHC-P Human 04/04/2020

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Breast cancer tissue
• Species: Human
• Lot: 4586-1P1170303

reviewed by: Verified Customer WB Mouse 12/27/2019

Summary

• Application: Western Blot
• Sample Tested: EMT6 cells
• Species: Mouse
• Lot: 4586-1p1170303

reviewed by: Verified Customer WB Mouse 02/18/2019

Summary

• Application: Western Blot
• Sample Tested: CT26,EMT6 cells
• Species: Mouse
• Lot: 4586-1P1170303

reviewed by: Verified Customer WB Human 10/17/2018

Summary

• Application: Western Blot
• Sample Tested: AGS human gastric adenocarcinoma cell line,KATO-III human gastric carcinoma cell line
• Species: Human
• Lot: 4586-1P1170303

FAQs for MUC5AC Antibody (NBP2-15196). (Showing 1 - 1 of 1 FAQs).

1. I would be interested in buying the anti-Mucin 5AC (clone 45M1). However, the antibodies from Abcam (your competitor), also clone "45M1" does not work in the rat. On your datasheet, you announce your antibody "cross React" with different species, including rats. Can you please confirm me?
   • Our Mucin 5AC antibody NBP2-15196 has rat and other species listed as possible cross reactive species based on homology. The Abcam antibody of the same clone also lists rat as a potential species. Unlike Abcam, we will apply our guarantee to species that have 90% homology or higher.

Bioinformatics

• Gene Symbol: MUC5AC
• Uniprot:
  • P98088(Human)