MUC4 Antibody

Images

 
Western Blot: MUC4 Antibody [NBP1-52193] - MUC4 is shown in the green channel, with beta-tubulin in red channel and Precision Plus dual color protein ladder in the left. The main band is about 60 kDa, with another band ...read more
Immunocytochemistry/ Immunofluorescence: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in HepG2 cells with FITC (green). Nuclei were counterstained with DAPI (blue).
Immunohistochemistry: MUC4 Antibody [NBP1-52193] - Staining of MUC4 in mouse prostate
Western Blot: MUC4 Antibody [NBP1-52193] - Analysis of MUC4 beta in MCF7 whole cell extract (A) and HeLa whole cell extract (B).
Simple Western: MUC4 Antibody [NBP1-52193] - Image shows a specific band for MUC4 in 1.0 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.56 mg/ml

Order Details

MUC4 Antibody Summary

Immunogen
A genomic peptide made to an internal region of the human MUC4 protein (within residues 1950-2100). [Swiss-Prot Q99102]
Localization
MUC4 beta chain: Cell membrane; Single-pass membrane protein.
Specificity
This antibody is specific for the beta chain of MUC4.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MUC4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot
  • Simple Western 1:500
  • Immunocytochemistry/Immunofluorescence 1:250
  • Immunohistochemistry 1:400
  • Immunohistochemistry-Paraffin 1:400
Application Notes
In Western blot bands are seen ~78 kDa representing MUC4 beta and ~50 kDa. The identity of the 50 kDa band is unknown. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended. Does not work for IP as per customer feedback.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Control
HeLa Whole Cell Lysate
MCF-7 Whole Cell Lysate
Reviewed Applications
Read 1 Review rated 4
using
NBP1-52193 in the following applications:

Publications
Read Publications using
NBP1-52193 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS, 0.1% BSA, 50% Glycerol
Preservative
0.05% Sodium Azide
Concentration
0.56 mg/ml
Purity
Immunogen affinity purified

Notes

Manufactured by Genomic Antibody Technology™. GAT FAQs

Alternate Names for MUC4 Antibody

  • Ascites sialoglycoprotein
  • ASGP
  • HSA276359
  • MUC4
  • MUC-4
  • mucin 4, cell surface associated
  • mucin 4, tracheobronchial
  • mucin-4
  • Pancreatic adenocarcinoma mucin
  • Testis mucin
  • Tracheobronchial mucin

Background

MUC4 (Mucin-4) is a large transmembrane protein that belongs to mucins family which contains high-molecular weight glycoproteins with oligosaccharides attached to serine or threonine residues of mucin core protein backbone by O-glycosidic linkages. Mucins have two catagories: transmembrane mucins (MUC1, MUC3, MUC4, MUC12, MUC13, MUC15, MUC16, MUC17, MUC20 and MUC21) and secreted mucins (MUC2, MUC5AC, MUC5B, MUC6, MUC7, and MUC19). Mucins are produced by epithelial cells and serve protective as well as lubricating role, however, in damaged epithelia or tumor cells, there is a loss of polarity that occurs in association with activation of proliferation/survival mechanisms. MUC4 is normally expressed in many epithelial tissues such as respiratory, colonic, and vaginal epithelia, and its expression is altered in various tumors including fibromyoxoid sarcoma and carcinomas of pancreas, bile duct, pancreatic duct etc. MUC4's mediated activation of ErbB2 downstream signaling may lead to cell proliferation/survival, and tumor cells can exploit versatile anti-apoptotic activities of MUC4 for augmenting survival or developing resistance to therapeutic agents. MUC4 has also been shown to enhance invasion and metastasis in prostate cancer, partly through regulating the EMT process (N-cadherin upregulation) and stabilizing FGFR1.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MUC4 Antibody (NBP1-52193)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, WB.


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ICC/IF
(1)
WB
(1)
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Human
(2)
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Review for MUC4 Antibody (NBP1-52193) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.

Reviews using NBP1-52193:
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WB
(1)
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Filter by Species
Mouse
(1)
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Images Ratings Applications Species Date Details
Western Blot MUC4 NBP1-52193
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reviewed by:
Anonymous
WB Mouse 01/24/2017
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Summary

ApplicationWestern Blot
Sample Testedmouse lung homogenate
SpeciesMouse

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC4 Antibody (NBP1-52193) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for MUC4 Antibody (NBP1-52193)

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Blogs on MUC4.

MUC4 (Mucin-4)
Mucus is the viscous secretion that covers epithelial surfaces (trachea, colon, and cervix) and consists of twenty highly glycosylated proteins called mucins. The mucin family all are high-molecular weight proteins with oligosaccharides attached to th...  Read full blog post.

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Recent Reviews

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1
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Anonymous
01/24/2017
Application: WB
Species: Mouse

Bioinformatics

Gene Symbol MUC4
Entrez
Uniprot