MUC4 Antibody (HL2026) - Azide and BSA Free

Western Blot: MUC4 Antibody (HL2026) - Azide and BSA Free [NBP3-25569] - Various whole cell extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (NBP3-25569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MUC4 Antibody (HL2026) - Azide and BSA Free [NBP3-25569] - Non-transfected (-) and transfected (+) 293T whole cell extracts were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (NBP3-25569) diluted at 1:100000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: MUC4 Antibody (HL2026) - Azide and BSA Free [NBP3-25569] - Various tissue extracts (30 ug) were separated by 5% SDS-PAGE, and the membrane was blotted with Mucin 4 X + Mucin 4 Y antibody [HL2026] (NBP3-25569) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Immunocytochemistry/Immunofluorescence: MUC4 Antibody (HL2026) - Azide and BSA Free [NBP3-25569] - Mucin 4 X + Mucin 4 Y antibody [HL2026] detects Mucin 4 X + Mucin 4 Y protein at cell membrane by immunofluorescent analysis. Sample: NCI-H441 cells were fixed in ice-cold MeOH for 5 min. Green: Mucin 4 X + Mucin 4 Y stained by Mucin 4 X + Mucin 4 Y antibody [HL2026] (NBP3-25569) diluted at 1:500. Blue: Fluoroshield with DAPI .

• Reactivity: Hu
• Applications: WB, ICC/IF
• Clone: HL2026
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MUC4 Antibody (HL2026) - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit MUC4 Antibody (HL2026) - Azide and BSA Free (NBP3-25569) is a recombinant monoclonal antibody validated for use in WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human MUC4 X + MUC4 Y.
• Specificity: Mucin 4 X + Mucin 4 Y
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MUC4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot 1:500-1:100000

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: No Preservative
• Purity: Protein A purified

Alternate Names for MUC4 Antibody (HL2026) - Azide and BSA Free

• Ascites sialoglycoprotein
• ASGP
• HSA276359
• MUC4
• MUC-4
• mucin 4, cell surface associated
• mucin 4, tracheobronchial
• mucin-4
• Pancreatic adenocarcinoma mucin
• Testis mucin
• Tracheobronchial mucin

Background

MUC4 is a constituents of mucus, the viscous secretion that covers epithelial surfaces such as those in the trachea, colon, and cervix, and is a highly glycosylated proteins. These glycoproteins play important roles in the protection of the epithelial cells and have been implicated in epithelial renewal and differentiation. MUC4 antibody studies have shown that MUC4 encodes an integral membrane glycoprotein found on the cell surface, although secreted isoforms may exist. At least two dozen transcript variants of MUC4 have been found, although for many of them the full-length transcript has not been determined or they are found only in tumor tissues. MUC4 is expressed in a wide variety of carcinomas making MUC4 antibodies a key cancer research tool.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MUC4