MUC2 Antibody (CCP58)

Images

 
Western Blot: MUC2 Antibody (CCP58) [NBP2-25221] - MDA MB 231 cells and HCC827 human non-small cell lung cancer cell line lysates. Image from verified customer review.
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-paraffin normal human intestine stained with MUC2 Ab (CCP58).
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (10X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.
Immunohistochemistry-Paraffin: MUC2 Antibody (CCP58) [NBP2-25221] - Formalin-fixed, paraffin-embedded normal human intestine (20X) stained with MUC2 antibody (1:200), peroxidase-conjugate and DAB chromogen.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP
Clone
CCP58
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
0.2 mg/ml

Order Details

View Available Conjugates
View Available Formulations
Catalog# & Formulation Size Price

MUC2 Antibody (CCP58) Summary

Immunogen
A synthetic peptide of 29 amino acids, KYPTTTPISTTTMVTPTPTPTGTQTPTTT from MUC2 protein, coupled to KLH.
Localization
Cytoplasmic and Cell surface
Specificity
Recognizes a single glycoprotein of 520kDa, identified as mucin 2 (MUC2). This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC3. Mucins are high molecular weight glycoproteins, which constitute the major component of the mucus layer that protects the gastric epithelium. MUC2 is specifically expressed in goblet cells of the small intestine & colon; in about 65% of colonic carcinomas and about 40% of gastric carcinomas. MUC2 is rarely expressed outside of the GI tract with the exceptions of mucinous carcinoma of breast and clear cell-type carcinomas of the ovary.
Isotype
IgG1 Kappa
Clonality
Monoclonal
Host
Mouse
Gene
MUC2
Purity
Protein A or G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-1ug/ml
  • Flow Cytometry 0.5-1ug/million cells
  • Immunocytochemistry/Immunofluorescence 1-2ug/ml
  • Immunohistochemistry-Frozen 0.5-1ug/ml
  • Immunohistochemistry-Paraffin 0.5-1ug/ml
  • Immunoprecipitation 1-2ug/500ug protein lysate
Application Notes
Flow Cytometry, Immunoprecipitation and Western Blot applications reported in literature.
Theoretical MW
520 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP2-25221 in the following applications:

Publications
Read Publications using
NBP2-25221 in the following applications:

  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C.
Buffer
PBS with 0.05% BSA.
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Purity
Protein A or G purified

Alternate Names for MUC2 Antibody (CCP58)

  • Intestinal mucin-2
  • MLP
  • MUC-2
  • mucin 2, intestinal/tracheal
  • mucin 2, oligomeric mucus/gel-forming
  • mucin-2
  • mucin-like protein
  • SMUC

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB120-22711
Species: Hu, Mu
Applications: ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
NBP2-15196
Species: Hu, Mu, Rt, Po, Ch, Fe, Pm, Rb, Bv(-)
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, Flow-IC
NBP2-44374
Species: Hu
Applications: Flow, IHC-P, IF
NBP1-92151
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NBP1-52193
Species: Hu, Mu
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
NBP1-85599
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-44434
Species: Hu, Rt(-)
Applications: Flow, IHC-P, IF
NBP2-24583
Species: Hu, Mu, Rt, Bv, Eq, Op, Pm
Applications: WB, IHC-P
AF1182
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ELISA(Sta)
NBP1-88080
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP1-76514
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC, IHC-P, ICC, IF
NBP1-06983
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC-P, IP, PEP-ELISA
NBP1-82045
Species: Hu
Applications: IHC, IHC-P
NBP1-84843
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF231
Species: Hu
Applications: WB, Flow, IHC, IP, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ELISA(Sta)
NBP2-25221
Species: Hu
Applications: WB, Flow, ICC/IF, IHC-Fr, IHC-P, IP

Publications for MUC2 Antibody (NBP2-25221)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC-P, WB.


Filter By Application
IHC-P
(1)
WB
(1)
All Applications
Filter By Species
Human
(2)
All Species

Review for MUC2 Antibody (NBP2-25221) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-25221:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot MUC2 NBP2-25221
Enlarge
reviewed by:
Anonymous
WB Human 10/17/2018
View

Summary

ApplicationWestern Blot
Sample TestedMDA MB 231 cells,HCC827 human non-small cell lung cancer cell line
SpeciesHuman
Lot4583-1P170511

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MUC2 Antibody (NBP2-25221) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Secondary Antibodies

 

Isotype Controls

Additional Array Products

Array NBP2-25221

Bioinformatics Tool for MUC2 Antibody (NBP2-25221)

Discover related pathways, diseases and genes to MUC2 Antibody (NBP2-25221). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MUC2 Antibody (NBP2-25221)

Discover more about diseases related to MUC2 Antibody (NBP2-25221).
 

Pathways for MUC2 Antibody (NBP2-25221)

View related products by pathway.

PTMs for MUC2 Antibody (NBP2-25221)

Learn more about PTMs related to MUC2 Antibody (NBP2-25221).

Blogs on MUC2

There are no specific blogs for MUC2, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Anonymous
10/17/2018
Application: WB
Species: Human

Bioinformatics

Gene Symbol MUC2
Uniprot