mtTFA Antibody (S08-3E2)

Western Blot: mtTFA Antibody (S08-3E2) [NBP3-19504] - Western blot detection of mtTFA in K562, Hela cell lysates using NBP3-19504 (1:1000 diluted). Predicted band size: 29kDa. Observed band size: 24kDa.

Immunocytochemistry/Immunofluorescence: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunofluorescence of mtTFA (green) in Hela cells using NBP3-19504 at dilution 1/200, and DAPI (blue)

Immunohistochemistry-Paraffin: mtTFA Antibody (S08-3E2) [NBP3-19504] - Immunohistochemistry of mtTFA in paraffin-embedded Human breast cancer tissue using NBP3-19504 at dilution 1/1

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clone: S08-3E2
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.3 mg/ml

mtTFA Antibody (S08-3E2) Summary

• Description: Novus Biologicals Rabbit mtTFA Antibody (S08-3E2) (NBP3-19504) is a recombinant monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide of human mtTFA (Uniprot # Q00059)
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: TFAM
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1/20-1/50
  • Immunohistochemistry 1/50
  • Immunohistochemistry-Paraffin
  • Western Blot 1/2000-1/10000
• Theoretical MW: 24 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 50mM Tris-Glycine(pH 7.4), 0.15M NaCl, 40% Glycerol, 0.05% BSA
• Preservative: 0.01% Sodium Azide
• Concentration: 0.3 mg/ml
• Purity: Affinity purified

Alternate Names for mtTFA Antibody (S08-3E2)

• mitochondrial transcription factor A
• MtTF1
• mtTFA
• TCF-6
• TCF6L1
• TCF6L2Mitochondrial transcription factor 1
• TCF6TCF6L3
• Transcription factor 6
• Transcription factor 6-like 2 (mitochondrial transcription factor)
• Transcription factor 6-like 2
• transcription factor A, mitochondrial

Background

TFAM a mitochondrial transcription factor that is a key activator of mitochondrial transcription as well as a participant in mitochondrial genome replication. Studies in mice have demonstrated that this protein is required to regulate the mitochondrial genome copy number and is essential for embryonic development. A mouse model for Kearns-Sayre syndrome was produced when expression of the gene was eliminated by targeted disruption in heart and muscle cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: TFAM