MTMR15 Antibody

Western Blot: MTMR15 Antibody [NBP1-42677] - Detection of human MTMR15 by western blot. Samples: Whole cell lysate (50 ug) from GaMG, HeLa, Jurkat, and My-La CD4+ cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MTMR15 antibody NBP1-42677 used for WB at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Immunohistochemistry-Paraffin: MTMR15 Antibody [NBP1-42677] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-MTMR15 (NBP1-42677) used at a dilution of 1:200 (1ug/ml). Detection: DAB

Immunoprecipitation: MTMR15 Antibody [NBP1-42677] - Detection of human MTMR15 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MTMR15 antibody NBP1-42677 used for IP at 6 ug per reaction. For blotting immunoprecipitated MTMR15, NBP1-42677 was used at 0.04 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

• Reactivity: Hu
• Applications: WB, IHC, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

MTMR15 Antibody Summary

• Description: Novus Biologicals Rabbit MTMR15 Antibody (NBP1-42677) is a polyclonal antibody validated for use in IHC, WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen for this product maps to a region between residue 1 and 50 of human myotubularin related protein 15 (NP_055782.3).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: FAN1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
  • Immunoprecipitation 2-10 ug/mg lysate
  • Western Blot 1:2000-1:10000
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTMR15 Antibody

• DKFZp451H236
• EC 3.1.21.-
• EC 3.1.4.1
• FANCD2/FANCI-associated nuclease 1DKFZp686K16147
• fanconi anemia associated nuclease 1
• fanconi-associated nuclease 1
• KIAA1018coiled-coil domain-containing protein MTMR15
• MTMR15
• myotubularin related protein 15
• Myotubularin-related protein 15

Background

The MTMR15 gene encodes a member of the myotubularin-related class 1 cysteine-based protein tyrosine phosphatases. The encoded protein may be catalytically inactive. Alternatively spliced transcript variants have been described. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for MTMR15 Antibody (NBP1-42677) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 11/15/2011

Summary

• Application: Western Blot
• Sample Tested: cell
• Species: Human

Bioinformatics

• Gene Symbol: FAN1
• Entrez:
  • 22909(Human)
• Uniprot:
  • Q9Y2M0(Human)