SLX4 Antibody - BSA Free

Images

 
Immunocytochemistry/ Immunofluorescence: BTBD12 Antibody [NBP1-28680] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were ...read more
Immunohistochemistry-Paraffin: BTBD12 Antibody [NBP1-28680] - IHC analysis of a formalin fixed and paraffin embedded tissue section of human breast infiltrating duct carcinoma using BTBD12 antibody at 1:250 dilution. ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ChIP, ICC/IF, IHC, IP, ChIP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

SLX4 Antibody - BSA Free Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1784 to 1834 of human SLX4 using the numbering given in entry NP_115820.2 (GeneID 84464).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SLX4
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Chromatin Immunoprecipitation (ChIP)
  • Chromatin Immunoprecipitation reported in scientific literature (PMID 24012755)
  • Immunocytochemistry/ Immunofluorescence 5 - 10 ug/ml. Use reported in scientific literature (PMID 24012755)
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
  • Immunoprecipitation 10 ug/mg lysate
  • Western Blot reported in scientific literature (PMID 24012755)
Application Notes
Antibody has not performed satisfactorily when used for WB of BTBD12 in crude preparations (e.g. whole cell lysate). This antibody can be used for WB of enriched (e.g. immunoprecipitated) sources of BTBD12. For IHC: Epitope retrieval with citrate buffer pH 6.0 is recommended.
Publications
Read Publications using
NBP1-28680 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SLX4 Antibody - BSA Free

  • BTB (POZ) domain containing 12
  • BTB/POZ domain-containing protein 12
  • BTBD12
  • BTBD12structure-specific endonuclease subunit SLX4
  • FANCP
  • KIAA1784
  • KIAA1784MUS312
  • KIAA1987
  • KIAA1987FANCP
  • SLX4 structure-specific endonuclease subunit homolog (S. cerevisiae)
  • SLX4

Background

BTBD12 (BTBD12 domain-containing protein 12) has been identified as the human ortholog of the yeast Slx4p and drosophila MUS312 DNA repair factors. BTBD12 is an ATM/ATR checkpoint substrate that functions as part of a Holliday junction resolvase and is important for DNA interstrand crosslink repair.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for SLX4 Antibody (NBP1-28680)(8)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 3 applications: Chemotaxis, ICC/IF, WB.


Filter By Application
Chemotaxis
(1)
ICC/IF
(1)
WB
(6)
All Applications
Filter By Species
Human
(5)
All Species
Showing Publications 1 - 8 of 8.
Publications using NBP1-28680 Applications Species
Stok C Mitotic processing of DNA replication and repair intermediates in cancer University of Groningen 2023-05-23 (WB, Human) WB Human
Sung S, Kim E, Niida H et al. Distinct characteristics of two types of alternative lengthening of telomeres in mouse embryonic stem cells Nucleic acids research 2023-07-27 [PMID: 37496110] (WB) WB
Heijink AM, Stok C, Porubsky D et al. Sister chromatid exchanges induced by perturbed replication can form independently of BRCA1, BRCA2 and RAD51 Nature communications 2022-11-07 [PMID: 36344511] (WB, Human)

Details:
Dilution used in ICC 1:1000
WB Human
Higa M, Matsuda Y, Fujii J et al. TRF2-mediated ORC recruitment underlies telomere stability upon DNA replication stress Nucleic Acids Research 2021-12-02 [PMID: 34761263] (WB) WB
Sadhukan R, Ghosh U PARP1 modulates telomere sister chromatid exchange and telomere length homeostasis by regulating telomere localization of SLX4 in U2OS cells Life Sciences 2021-05-01 [PMID: 33945829]
Sabatella M, Pines A, Slyskova J et al. ERCC1-XPF targeting to psoralen-DNA crosslinks depends on XPA and FANCD2 Cell. Mol. Life Sci. 2019-08-07 [PMID: 31392348] (WB, Human) WB Human
Sadhukhan R, Chowdhury P, Ghosh S, Ghosh U. Expression of Telomere-Associated Proteins is Interdependent to Stabilize Native Telomere Structure and Telomere Dysfunction by G-Quadruplex Ligand Causes TERRA Upregulation Cell Biochem. Biophys. 2017-11-13 [PMID: 29134494] (Human) Human
Wan B, Yin J, Horvath K et al. SLX4 Assembles a Telomere Maintenance Toolkit by Bridging Multiple Endonucleases with Telomeres. Cell Rep. 2013-09-12 [PMID: 24012755] (ICC/IF, WB, Chemotaxis, Human) ICC/IF, WB, Chemotaxis Human

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Product General Protocols

Video Protocols

WB Video Protocol
ChIP Webinar
ChIP Video Protocol
ICC/IF Video Protocol

FAQs for SLX4 Antibody (NBP1-28680) (0)

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Secondary Antibodies

 

Isotype Controls

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FANCD2: A big component of the DNA repair crew
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Bioinformatics

Gene Symbol SLX4
Entrez
Uniprot