MTCH2 Antibody - Azide and BSA Free

Western Blot: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Western blot analysis of lysates from wild type(WT) and MTCH2 knockout (KO) HeLa(KO) cells, using [KO Validated] MTCH2 Rabbit pAb (A12934) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25μg per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3s.

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Immunofluorescence analysis of NIH-3T3 cells using [KO Validated] MTCH2 Rabbit pAb (A12934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunocytochemistry/ Immunofluorescence: MTCH2 Antibody - Azide and BSA Free [NBP2-94185] - Immunofluorescence analysis of U-2 OS cells using [KO Validated] MTCH2 Rabbit pAb (A12934) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free

MTCH2 Antibody - Azide and BSA Free Summary

• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-175 of human MTCH2 (NP_055157.1). VGYEPLPPTIGRNIFGRQVCQLPGLFSYAQHIASIDGRRGLFTGLTPRLCSGVLGTVVHGKVLQHYQESDKGEELGPGNVQKEVSSSFDHVIKETTREMIARSAATLITHPFHVITLRSMVQFIGRESKYCGLCDSIITIYREEGI
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MTCH2
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry 1:50-1:200
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 33 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.01% Thimerosal
• Purity: Affinity purified

Alternate Names for MTCH2 Antibody - Azide and BSA Free

• 2310034D24Rik
• Met-induced mitochondrial protein
• MIMP
• mitochondrial carrier 2
• mitochondrial carrier homolog 2 (C. elegans)
• mitochondrial carrier homolog 2

Background

The substrate transported is not yet known. Induces mitochondrial depolarization

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

Bioinformatics

• Gene Symbol: MTCH2