MTA1 Antibody

Western Blot: MTA1 Antibody [NB100-823] - Staining of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Immunohistochemistry-Paraffin: MTA1 Antibody [NB100-823] - Staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunohistochemistry-Paraffin: MTA1 Antibody [NB100-823] - Staining of paraffin embedded Human Adrenal Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

MTA1 Antibody Summary

• Description: Novus Biologicals Goat MTA1 Antibody (NB100-823) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-MTA1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-PGDVFYMPKE corresponding to internal region (near C-Terminus) according to AAA78935.1.
• Localization: Cytoplasmic (short isoform) or nuclear (long isoform).
• Specificity: Immunizing peptide was designed based on NP_004680.1. The version changed to NP_004680.2 and no longer matches with the peptide.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: MTA1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3.75 ug/ml
  • Immunohistochemistry-Paraffin 3.75 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 75 kDa band observed in human placenta lysates (calculated MW of 80.8 kDa band according to NP_004680.1). IHC: Paraffin embedded Human Testis and Adrenal Gland.
• Control:

  Human Placenta Whole Tissue Lysate (Adult Whole Normal)
  MTA1 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for MTA1 Antibody

• metastasis associated 1
• metastasis associated gene 1 protein
• metastasis associated protein
• metastasis-associated protein MTA1

Background

MTA1 is a nuclear regulatory factor, implicated in cellular transformation, in part through its ability to regulate the epithelial-to-mesenchymal transition and metastasis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MTA1 Antibody (NB100-823)(2)

Publications using NB100-823

• V Sharma, M Makhdoomi, L Singh, P Kumar, N Khan, S Singh, HN Verma, K Luthra, S Sarkar, D Kumar Trehalose limits opportunistic mycobacterial survival during HIV co-infection by reversing HIV-mediated autophagy block Autophagy, 2020-02-20;0(0):1-20. 2020-02-20 [PMID: 32079455]
• Toh Y, Pencil SD, Nicolson GL. A novel candidate metastasis-associated gene, mta1, differentially expressed in highly metastatic mammary adenocarcinoma cell lines. cDNA cloning, expression, and protein analyses. J Biol Chem 1994-09-16 [PMID: 8083195]

Bioinformatics

• Gene Symbol: MTA1
• Entrez:
  • 9112(Human)
• Uniprot:
  • NP_004680.1(Human)