MSH6 Antibody

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Biological Strategies: Western Blot: MSH6 Antibody [NB100-329] - Detection of Human MSH6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry: MSH6 Antibody [NB100-329] - Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti- MSH6 used at a dilution of 1:500 (2ug/ml). Detection: DAB. Counterstain: IHC ...read more
Western Blot: MSH6 Antibody [NB100-329] - Whole cell lysate (30 ug) from human lymphoblast TK6 (wildtype MSH6 expression) or MT1 (TK6 derived cells with reduced expression of a mutant MSH6). Antibody used at 0.2 ug/ml.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IP, RIA
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
   

Biological Strategies

     

Order Details

MSH6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1300 and the C-terminus (residue 1360) of human MutS homolog 6 using the numbering given in entry NP_000170.1 (GeneID 2956).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
MSH6
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:2500-1:10000
  • Immunohistochemistry
  • Immunoprecipitation 1-4 ug/mg lysate
  • Radioimmunoassay
Application Notes
Uses: NB 100-329 may be used for Western Blot and immunoprecipitation. Suggested working dilutions: * WB - 1:2,500-1:10,000 (ECL) IP - 1 to 4 ug/mg of lysate IHC / ICC - Not Determined *The investigator should determine the optimal working dilution for a specific application.
Control
MSH6 Lysate (NBL1-13328)
MSH6 Lysate (NBP2-64905)
Publications
Read Publications using NB100-329.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for MSH6 Antibody

  • DNA mismatch repair protein Msh6
  • G/T mismatch-binding protein
  • GTBPHNPCC5
  • GTMBP
  • hMSH6
  • HSAP
  • mutS (E. coli) homolog 6
  • mutS homolog 6 (E. coli)
  • MutS-alpha 160 kDa subunit
  • p160
  • sperm-associated protein

Background

MSH6 is a mismatch-repair protein. It plays a role in tumor suppression. Genomic mutations in MSH6 predispose to the hereditary non-polyposis colorectal cancer (HNPCC) syndrome.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MSH6 Antibody (NB100-329)(2)

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Video Protocols

WB Video Protocol

FAQs for MSH6 Antibody (NB100-329). (Showing 1 - 1 of 1 FAQs).

  1. I am testing a mouse sample with human xenograft for GBM and I am looking for an MSH6 Antibody that will only detect the human cells.
    • Currently, we have no validation that any of our MSH6 antibodies will not cross react with mouse. All of our MSH6 Antibodies are raised against Human MSH6 so we have checked the % homology with mouse MSH6 which has come to be 85%. As 85% is very high, the chances of the antibody detecting the mouse tissue as well as the human tissue are high.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol MSH6
Entrez
Uniprot