MRRF Antibody (1T5W1)

Western Blot: MRRF Antibody (1T5W1) [NBP3-16134] - Western blot analysis of extracts of various cell lines, using MRRF antibody (NBP3-16134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Western Blot: MRRF Antibody (1T5W1) [NBP3-16134] - Western blot analysis of extracts of 293T cells, using MRRF antibody (NBP3-16134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

• Reactivity: Hu, Mu, Rt
• Applications: WB
• Clone: 1T5W1
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

MRRF Antibody (1T5W1) Summary

• Description: Novus Biologicals Rabbit MRRF Antibody (1T5W1) (NBP3-16134) is a recombinant monoclonal antibody validated for use in WB. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 163-262 of human MRRF (Q96E11). RESGMNLNPEVEGTLIRVPIPQVTREHREMLVKLAKQNTNKAKDSLRKVRTNSMNKLKKSKDTVSEDTIRLIEKQISQMADDTVAELDRHLAVKTKELLG
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: MRRF
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:500 - 1:1000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MRRF Antibody (1T5W1)

• mitochondrial ribosome recycling factor
• mitochondrial
• MTRRF

Background

Responsible for the release of ribosomes from messenger RNA at the termination of protein biosynthesis. Mayincrease the efficiency of translation by recycling ribosomes from one round of translation to another

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRRF