MRPS30 Antibody - BSA Free

Western Blot: MRPS30 Antibody [NBP2-94408] - Analysis of extracts of various cell lines, using MRPS30 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 90s.

Western Blot: MRPS30 Antibody [NBP2-94408] - Analysis of 200ug extracts of HepG2 cells using MRPS30 at a dilition of 1:1000.

Immunocytochemistry/ Immunofluorescence: MRPS30 Antibody - BSA Free [NBP2-94408] - Immunofluorescence analysis of U2OS cells using MRPS30 Rabbit pAb (A5841). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ICC/IF, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

MRPS30 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit MRPS30 Antibody - BSA Free (NBP2-94408) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 190-439 of human MRPS30 (NP_057724.2). PHNPALAAAALDYRCPVHFYWVRGEEIIPRGHRRGRIDDLRYQIDDKPNNQIRISKQLAEFVPLDYSVPIEIPTIKCKPDKLPLFKRQYENHIFVGSKTADPCCYGHTQFHLLPDKLRRERLLRQNCADQIEVVFRANAIASLFAWTGAQAMYQGFWSEADVTRPFVSQAVITDGKYFSFFCYQLNTLALTTQADQNNPRKNICWGTQSKPLYETIEDNDVKGFNDDVLLQIVHFLLNRPKEEKSQLLEN
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MRPS30
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:100
  • Immunoprecipitation 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 50 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for MRPS30 Antibody - BSA Free

• 28S ribosomal protein S30, mitochondrial
• DKFZp566B2024
• mitochondrial ribosomal protein S30
• MRP-S30
• PAP
• PDCD9
• programmed cell death 9
• Programmed cell death protein 9
• S30mt

Background

MRPS30, or Mitochondrial Ribosomal Protein S30, consists of a 439 amino acid isoform that is 50 kDa, and is involved in mitochondrial protein synthesis in the heart, skeletal muscles, kidney, and liver. Current disease research is being conducted on MRPS30 and its relation to breast cancer, neuronitis, and prostatitis. MRPS30 has been linked to translation and apoptosis, through which it interacts with ICT1, MAGEA11, AARS2, AASS, and ABCB7.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: MRPS30