MPG Overexpression Lysate (Native)

Images

 
Western Blot: MPG Overexpression Lysate (Adult Normal) [NBL1-13197] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for MPG.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

MPG Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204933 and based on accession number NM_001015054. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens N-methylpurine-DNA glycosylase (MPG), transcript variant 3, mRNA.
Gene
MPG

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
30.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for MPG

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for MPG Overexpression Lysate (Native)

  • 3' end of the Mid1 gene, localized 68 kb upstream the humanzeta globin gene on16p
  • 3-alkyladenine DNA glycosylase
  • 3-methyladenine DNA glycosidase
  • AAG
  • ADPG
  • anpg
  • APNG
  • CRA36.1 (3-methyl-adenine DNA glycosylase)
  • CRA36.1
  • DNA-3-methyladenine glycosylase
  • EC 3.2.2.21
  • MDGalkyladenine DNA glycosylase
  • Mid1
  • N-methylpurine-DNA glycosylase, MPG
  • N-methylpurine-DNA glycosylasePIG16
  • PIG11
  • proliferation-inducing protein 11
  • proliferation-inducing protein 16

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB100-116
Species: Hu, Mu, Rt, Pm
Applications: WB, Simple Western, ChIP, GS, ICC/IF, IHC, IHC-Fr, IHC-P, IP, PLA
NB100-168
Species: Hu, Mu(-)
Applications: WB, Simple Western, Flow, ICC/IF, IHC, IHC-P, CyTOF-ready, Flow-IC
NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-45897
Species: Hu
Applications: WB, IHC
NB100-858
Species: Hu, Mu, Rt, GP, Mk
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, PEP-ELISA, IHC-WhMt
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NB200-103
Species: Hu, Mu, Rt, Ye, Xp(-)
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP, CyTOF-ready, Flow-IC
NBP2-16182
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-13075
Species: Hu, Mu, Rt
Applications: WB, ELISA, ICC/IF, IHC, IHC-P, IP, IHC-FrFl
H00008086-M02
Species: Hu, Mu, Rt
Applications: WB, ELISA, IHC-P
H00005018-D01P
Species: Hu
Applications: WB, ICC/IF
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP1-88866
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-106
Species: Hu, Mu, Rt, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB600-935
Species: Bv
Applications: WB, ELISA, IP
NBL1-13197
Species: Hu
Applications: WB

Publications for MPG Lysate (NBL1-13197) (0)

There are no publications for MPG Lysate (NBL1-13197).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for MPG Lysate (NBL1-13197) (0)

There are no reviews for MPG Lysate (NBL1-13197). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for MPG Lysate (NBL1-13197) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional MPG Products

Bioinformatics Tool for MPG Lysate (NBL1-13197)

Discover related pathways, diseases and genes to MPG Lysate (NBL1-13197). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for MPG Lysate (NBL1-13197)

Discover more about diseases related to MPG Lysate (NBL1-13197).
 

Pathways for MPG Lysate (NBL1-13197)

View related products by pathway.

PTMs for MPG Lysate (NBL1-13197)

Learn more about PTMs related to MPG Lysate (NBL1-13197).

Blogs on MPG

There are no specific blogs for MPG, but you can read our latest blog posts.

Customers Who Bought This Also Bought

MPG Antibody
NBP1-82787
OGG1 Antibody
NB100-106

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our MPG Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol MPG