Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse VEGFR2/Flk-1 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse soluble VEGF R2 Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse sVEGF R2 in cell culture supernates, tissue homogenates, serum, and plasma. It contains NS0-expressed recombinant mouse VEGF R2 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using n...atural mouse sVEGF R2 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse sVEGF R2.
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• Specificity: Natural and recombinant mouse sVEGF R2
• Source: N/A
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Kdr

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse VEGFR2/Flk-1 Quantikine ELISA Kit

• CD309 antigen
• CD309
• EC 2.7.10
• EC 2.7.10.1
• Fetal liver kinase 1
• fetal liver kinase-1
• Flk1
• Flk-1
• FLK1tyrosine kinase growth factor receptor
• KDR
• kinase insert domain receptor (a type III receptor tyrosine kinase)
• Kinase insert domain receptor
• KRD1
• Ly73
• Protein-tyrosine kinase receptor flk-1
• soluble VEGFR2
• vascular endothelial growth factor receptor 2
• VEGF R2
• VEGFR
• VEGFR2
• VEGFR-2

Background

VEGF R1 (Flt-1), VEGF R2 (KDR/Flk-1), and VEGF R3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domain and kinase insert domains in their intracellular region. They are best known for regulating VEGF family-mediated vasculogenesis, angiogenesis, and lymphangiogenesis. They are also mediators of neurotrophic activity and regulators of hematopoietic development. VEGF R2 is thought to be the primary inducer of VEGF-mediated blood vessel growth, while VEGF R3 plays a significant role in VEGF-C and VEGF-D-mediated lymphangiogenesis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for VEGFR2/KDR/Flk-1 (MVR200B)(14)

Publications using MVR200B

• M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695634] (Mouse)
• CM Medeiros D, V Perugini, P Bernegger, M Centola, A Barbero, AL Guildford, M Santin, A Banfi, I Martin, A Marsano Vascular Endothelial Growth Factor Sequestration Enhances In Vivo Cartilage Formation Int J Mol Sci, 2017-11-21;18(11):. 2017-11-21 [PMID: 29160845] (Human)
• C Di Mauro, R Rosa, V D'Amato, P Ciciola, A Servetto, R Marciano, RC Orsini, L Formisano, S De Falco, V Cicatiello, M Di Bonito, M Cantile, F Collina, A Chambery, BM Veneziani, S De Placido, R Bianco Hedgehog signalling pathway orchestrates angiogenesis in triple-negative breast cancers Br. J. Cancer, 2017-04-25;0(0):. 2017-04-25 [PMID: 28441382] (Human, Mouse)
• Howell KR, Kutiyanawalla A, Pillai A Long-term continuous corticosterone treatment decreases VEGF receptor-2 expression in frontal cortex. PLoS ONE, 2011-05-27;6(5):e20198. 2011-05-27 [PMID: 21647420] (Mouse)
• Ruiz de Almodovar C, Coulon C, Salin PA, Knevels E, Chounlamountri N, Poesen K, Hermans K, Lambrechts D, Van Geyte K, Dhondt J, Dresselaers T, Renaud J, Aragones J, Zacchigna S, Geudens I, Gall D, Stroobants S, Mutin M, Dassonville K, Storkebaum E, Jordan BF, Eriksson U, Moons L, D'Hooge R, Haigh JJ, Belin MF, Schiffmann S, Van Hecke P, Gallez B, Vinckier S, Chedotal A, Honnorat J, Thomasset N, Carmeliet P, Meissirel C Matrix-binding vascular endothelial growth factor (VEGF) isoforms guide granule cell migration in the cerebellum via VEGF receptor Flk1. J. Neurosci., 2010-11-10;30(45):15052-66. 2010-11-10 [PMID: 21068311] (Mouse)
• Satofuka S, Ichihara A, Nagai N, Noda K, Ozawa Y, Fukamizu A, Tsubota K, Itoh H, Oike Y, Ishida S (Pro)renin receptor promotes choroidal neovascularization by activating its signal transduction and tissue renin-angiotensin system. Am. J. Pathol., 2008-10-30;173(6):1911-8. 2008-10-30 [PMID: 18974301] (Mouse)
• Izumi-Nagai K, Nagai N, Ohgami K, Satofuka S, Ozawa Y, Tsubota K, Ohno S, Oike Y, Ishida S Inhibition of choroidal neovascularization with an anti-inflammatory carotenoid astaxanthin. Invest. Ophthalmol. Vis. Sci., 2008-04-01;49(4):1679-85. 2008-04-01 [PMID: 18385091] (Mouse)
• Xie B, Shen J, Dong A, Swaim M, Hackett SF, Wyder L, Worpenberg S, Barbieri S, Campochiaro PA An Adam15 amplification loop promotes vascular endothelial growth factor-induced ocular neovascularization. FASEB J., 2008-04-01;22(8):2775-83. 2008-04-01 [PMID: 18381816] (Mouse)
• Ebos JM, Lee CR, Bogdanovic E, Alami J, Van Slyke P, Francia G, Xu P, Mutsaers AJ, Dumont DJ, Kerbel RS Vascular endothelial growth factor-mediated decrease in plasma soluble vascular endothelial growth factor receptor-2 levels as a surrogate biomarker for tumor growth. Cancer Res., 2008-01-15;68(2):521-9. 2008-01-15 [PMID: 18199548] (Mouse)
• Ebos JM, Lee CR, Christensen JG, Mutsaers AJ, Kerbel RS Multiple circulating proangiogenic factors induced by sunitinib malate are tumor-independent and correlate with antitumor efficacy. Proc. Natl. Acad. Sci. U.S.A., 2007-10-17;104(43):17069-74. 2007-10-17 [PMID: 17942672] (Mouse)
• Shimojo N, Jesmin S, Zaedi S, Otsuki T, Maeda S, Yamaguchi N, Aonuma K, Hattori Y, Miyauchi T Contributory role of VEGF overexpression in endothelin-1-induced cardiomyocyte hypertrophy. Am. J. Physiol. Heart Circ. Physiol., 2007-03-16;293(1):H474-81. 2007-03-16 [PMID: 17369464] (Rat)
• Iemitsu M, Maeda S, Jesmin S, Otsuki T, Miyauchi T Exercise training improves aging-induced downregulation of VEGF angiogenic signaling cascade in hearts. Am. J. Physiol. Heart Circ. Physiol., 2006-04-14;291(3):H1290-8. 2006-04-14 [PMID: 16617130] (Rat)
• Nagai N, Noda K, Urano T, Kubota Y, Shinoda H, Koto T, Shinoda K, Inoue M, Shiomi T, Ikeda E, Tsubota K, Suda T, Oike Y, Ishida S Selective suppression of pathologic, but not physiologic, retinal neovascularization by blocking the angiotensin II type 1 receptor. Invest. Ophthalmol. Vis. Sci., 2005-03-01;46(3):1078-84. 2005-03-01 [PMID: 15728568] (Mouse)
• Maynard SE, Min JY, Merchan J, Lim KH, Mondal S, Stillman IE, Epstein FH, Karumanchi SA Excess placental soluble fms-like tyrosine kinase 1 (sFlt1) may contribute to endothelial dysfunction, hypertension, and proteinuria in preeclampsia. J. Clin. Invest., 2003-03-01;111(5):649-58. 2003-03-01 [PMID: 12618519] (Rat)

FAQs for VEGFR2/KDR/Flk-1 (MVR200B). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Kdr