Mouse TNF-alpha Quantikine ELISA Kit, 2 Plate

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Reactivity MuSpecies Glossary
Applications ELISA

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Mouse TNF-alpha Quantikine ELISA Kit, 2 Plate Summary

The Quantikine Mouse TNF-alpha Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse TNF-alpha levels in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse TNF-alpha and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant mouse TNF-alpha accurately. Results obtai...ned using natural mouse TNF-alpha showed dose-response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse TNF-alpha.
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Natural and recombinant mouse TNF-alpha.
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Spike Recovery
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Sample Volume
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Application Notes
Interference observed with 1 or more available related molecules.
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MTA00B in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse TNF-alpha Quantikine ELISA Kit, 2 Plate

  • APC1 protein
  • Cachectin
  • Cachetin
  • DIF
  • TNF
  • TNF, monocyte-derived
  • TNFA
  • TNF-A
  • TNFalpha
  • TNF-alpha
  • TNF-alphacachectin
  • TNFATNF, macrophage-derived
  • TNFG1F
  • TNFSF2
  • TNFSF2TNF superfamily, member 2
  • tumor necrosis factor (TNF superfamily, member 2)
  • tumor necrosis factor alpha
  • Tumor necrosis factor ligand superfamily member 2
  • tumor necrosis factor
  • tumor necrosis factor-alpha


Tumor necrosis factor alpha (TNF-alpha ), also known as cachectin and TNFSF1A, is the prototypic ligand of the TNF superfamily (1). It is a pleiotropic molecule that plays a central role in inflammation, immune system development, apoptosis, and lipid metabolism (2-5). TNF-alpha is also involved in a number of pathological conditions including asthma, Crohn's disease, rheumatoid arthritis, neuropathic pain, obesity, type 2 diabetes, septic shock, autoimmunity, and cancer (5-11).   
Mouse TNF-alpha is synthesized as a 26 kDa type II transmembrane protein that consists of a 35 amino acid (aa) cytoplasmic domain, a 21 aa transmembrane segment, and a 179 aa extracellular domain (ECD) (12). Within the ECD, mouse TNF-alpha shares 95% aa identity with rat, and 80% aa identity with canine, equine, feline, human, rabbit, and porcine TNF-alpha. It is produced by a wide variety of immune, epithelial, endothelial, and tumor cells. TNF-alpha is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface (13). Cell surface TNF-alpha can both induce the lysis of tumor cells and virus infected cells, and generate its own downstream cell signaling following ligation by soluble TNF RI (14, 15). Shedding of membrane bound TNF-alpha by TACE/ADAM17 releases the bioactive cytokine, a 55 kDa soluble trimer containing the TNF-alpha extracellular domain (16-18).    
TNF-alpha binds the ubiquitous 55-60 kDa TNF RI (19, 20) and the hematopoietic cell-restricted 78-80 kDa TNF RII (21, 22), both of which are also expressed as homotrimers (1, 23). Both type I and type II receptors bind TNF-alpha with comparable affinity and can promote NFkB activation (24-27). Only TNF RI, however, contains a cytoplasmic death domain which triggers the activation of apoptosis (3, 28). Soluble forms of both types of receptors are released into human serum and urine, and can neutralize the biological activity of TNF (29-31).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for TNF-alpha (MTA00B)(441)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, Equine.

We have publications tested in 1 application: ELISA Capture.

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Showing Publications 1 - 10 of 441. Show All 441 Publications.
Publications using MTA00B Applications Species
H Sun, Y Jiang, Y Song, X Zhang, J Wang, J Zhang, J Kang The MUC5B Mucin Is Involved in Paraquat-Induced Lung Inflammation Oxid Med Cell Longev, 2020;2020(0):7028947. 2020 [PMID: 32724493] (Mouse) Mouse
X Wu, S Pan, W Luo, Z Shen, X Meng, M Xiao, B Tan, K Nie, T Tong, X Wang Roseburia�intestinalis?derived flagellin ameliorates colitis by targeting miR?223?3p?mediated activation of NLRP3 inflammasome and pyroptosis Mol Med Rep, 2020;0(0):. 2020 [PMID: 32700754] (Mouse) Mouse
HF Zhang, HB Zhang, XP Wu, YL Guo, WD Cheng, F Qian Fisetin alleviates sepsis-induced multiple organ dysfunction in mice via inhibiting p38 MAPK/MK2 signaling Acta Pharmacol. Sin., 2020;0(0):. 2020 [PMID: 32661350] (Mouse) Mouse
B Lu, H Yuan, X Zhai, X Li, J Qin, J Chen, B Meng High-Pressure Pneumoperitoneum Aggravates Surgery-Induced Neuroinflammation and Cognitive Dysfunction in Aged Mice Mediators Inflamm., 2020;2020(0):6983193. 2020 [PMID: 32655313] (Mouse) Mouse
JY Kim, SJ Lee, YI Maeng, J Leem, KK Park Protective Effects of Bee Venom against Endotoxemia-Related Acute Kidney Injury in Mice Biology (Basel), 2020;9(7):. 2020 [PMID: 32640615] (Mouse) Mouse
F Chiuppesi, MD Salazar, H Contreras, VH Nguyen, J Martinez, S Park, J Nguyen, M Kha, A Iniguez, Q Zhou, T Kaltcheva, R Levytskyy, ND Ebelt, TH Kang, X Wu, T Rogers, ER Manuel, Y Shostak, DJ Diamond, F Wussow Development of a Synthetic Poxvirus-Based SARS-CoV-2 Vaccine bioRxiv, 2020;0(0):. 2020 [PMID: 32637957] (Mouse) Mouse
G de la Rosa, AI Gómez, MC Baños, P Pelegrín Signaling Through Purinergic Receptor P2Y2 Enhances Macrophage IL-1&beta Production Int J Mol Sci, 2020;21(13):. 2020 [PMID: 32630144] (Mouse) Mouse
J Banga, D Srinivasan, CC Sun, CD Thompson, F Milletti, KS Huang, S Hamilton, S Song, AF Hoffman, YG Qin, B Matta, M LaPan, Q Guo, G Lu, D Li, H Qian, DR Bolin, L Liang, C Wartchow, J Qiu, M Downing, S Narula, N Fotouhi, JA DeMartino, SL Tan, G Chen, BJ Barnes Inhibition of IRF5 cellular activity with cell-penetrating peptides that target homodimerization Sci Adv, 2020;6(20):eaay1057. 2020 [PMID: 32440537] (Human) Human
R Abohalaka, TE Bozkurt, E Nemutlu, SC Onder, I Sahin-Erde The Effects of Fatty Acid Amide Hydrolase and Monoacylglycerol Lipase Inhibitor treatments on Lipopolysaccharide-induced Airway Inflammation in Mice Pulm Pharmacol Ther, 2020;0(0):101920. 2020 [PMID: 32416152] (Mouse) Mouse
P Gurung, S Dahal, P Chaudhary, D Guragain, U Karmachary, JA Kim, BS Jeong Potent Inhibitory Effect of BJ-3105, a 6-Alkoxypyridin-3-ol Derivative, on Murine Colitis Is Mediated by Activating AMPK and Inhibiting NOX Int J Mol Sci, 2020;21(9):. 2020 [PMID: 32365634] (Mouse) Mouse
Show All 441 Publications.

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Product General Protocols

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FAQs for TNF-alpha (MTA00B). (Showing 1 - 5 of 5 FAQs).

  1. I need to know about TNF-alpha antibody kits for mice.
    • We currently have two TNFalpha ELISA kits specific for mouse: catalog numbers KA0257 and NBP1-92670.
  2. I would like to ask you for help. I need an antibody for Elisa to detect human TNF alpha in a supernatant from a cell culture (meaning supernatant after centrifugation of collected cell suspension from a plate well). Which of your antibodies against human tnf alpha would be suitable? I would like to buy only the primary antibody.
    • I would recommend catalog number NBP1-67821 or NB600-587; however, a full list of our anti-human TNF alpha antibodies suitable for use in ELISA can be found using this link.
  3. I am interested in a TNF alpha antibody, cross reactive for human, rat and mouse (host species: rabbit). Could your product NBP1-19532 be used in western blotting applications? Or do you have a similar product in your catalog which could fit with my request?
    • The antibody you mention, NBP1-19532, has not yet been validated in Western blot. I would instead recommend either NBP1-67821 or NB600-587. These are both rabbit polyclonal antibodies that cross-reacts with human, mouse and rat and have been used in Western blotting.
  4. Can your TNF-alpha products be used to treat TBI victims and therefore avoid the perispinal injections with Enbrel?
    • I am very sorry, but all of our products are for scientific research use only, and none are intended or approved for use in humans.
  5. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

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Blogs on TNF-alpha.

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Liver ASK1 activates autophagy to protect against hepatic fat accumulation, non-alcoholic steatohepatitis and fibrosis
By Jamshed Arslan, Pharm. D., PhD. The most common chronic liver disorder worldwide is non-alcoholic fatty liver disease (NAFLD). This obesity-linked disorder can manifest as hepatic fat accumulation (steatosis) wit...  Read full blog post.

Metabolic Syndrome: Symptoms and associated disease states
By Jamshed Arslan, Pharm. D., PhD. What is metabolic syndrome?It was 1988 when, after decades of research, Dr. GM Reaven    of the Stanford University, CA, explained the relationship between four diseases:...  Read full blog post.

Increased wild type FUS levels in ALS patients lead to a toxic microenvironment and motor neuron neurodegeneration
By Michalina Hanzel, PhDFUS mutations in Amyotrophic Lateral SclerosisFused in sarcoma (FUS) is a ribonucleoprotein that continuously shuttles between the nucleus and the cytoplasm to regulate pre-mRNA splicing, mRN...  Read full blog post.

You complete me: Natural killer cells need TGF-beta inhibition to effectively combat cancers
By Jamshed Arslan Pharm.D. Natural killer (NK) cells are lymphocytes of the innate immune system that were first discovered for their “natural” ability to kill cancer cells. To use NK cells as anti-cancer therapy, t...  Read full blog post.

Friends become Foes: Molecular Chaperons, Hsp70 and Hsp90, Cause Muscle Wasting in Cancers
By Jamshed Arslan Pharm.D. Muscle atrophy is a common feature of many tumors. Cancer-induced muscle wasting, or cancer cachexia, results from pro-in?ammatory cytokines (TNFa and IL-6) and/or agonists of type IIB ac...  Read full blog post.

cIAP2 - balancing cell death and cell survival
The inhibitor of apoptosis proteins (IAPs) are important regulators of cell death and inflammation. The cellular inhibitor of apoptosis protein 2 (cIAP2) contains three Baculovirus IAP repeat (BIR) domains, a Ubiquitin associated (UBA) domain, and...  Read full blog post.

CD86 - I work in tandem with CD80
CD86 belongs to the immunoglobulin superfamily of proteins that drive innate and adaptive immune responses. It is an 80kD co-stimulatory molecule for the priming and activation of naive and memory T-cells, respectively. CD86 is expressed on activated...  Read full blog post.

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Gene Symbol Tnf