Mouse S100A8 DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse S100A8 DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: S100a8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse S100A8 DuoSet ELISA, 15 Plate

• 60B8AG
• CAGACP-10
• Calgranulin A
• calgranulin-A
• Calprotectin L1L subunit
• CFAG
• CFAGL1Ag
• CGLA
• Cystic fibrosis antigen
• Leukocyte L1 complex light chain
• MA387
• MIF
• Migration inhibitory factor-related protein 8
• MRP8
• MRP-8
• MRP8S100 calcium binding protein A8 (calgranulin A)
• NIF
• P8
• protein S100-A8
• S100 calcium binding protein A8
• S100 calcium-binding protein A8 (calgranulin A)
• S100 calcium-binding protein A8
• S100A8
• Urinary stone protein band A

Background

S100A8 (also MRP8 and calgranulin A) is a 10 kDa member of the S100 family, EF-hand superfamily of Ca-binding proteins. It is produced by neutrophils and monocytes, and forms Ca²⁺-dependent heterodimer/heterotetramer complexes (termed calprotectin) with S100A9. It functions both intracellularly and extracellularly, where it binds to RAGE and CD36. Human S100A8 is 93 amino acids (aa) in length. It contains two EF-hand motifs (aa 12 - 47 and 46 - 81) and one high-affinity Ca²⁺-binding site (aa 59 - 70). There may be one splice form that shows a 15 aa substitution for the C-terminal 14 amino acids. Although mouse S100A8 is cleaved by MMP-2 after Asn21, it is unclear if human S100A8 is susceptible. Full-length human S100A8 is 57% and 74% aa identical to mouse and canine S100A8, respectively.

Publications for S100A8 (DY3059)(12)

Publications using DY3059

• Kurtz, SL;Baker, RE;Boehm, FJ;Lehman, CC;Mittereder, LR;Khan, H;Rossi, AP;Gatti, DM;Beamer, G;Sassetti, CM;Elkins, KL; Multiple genetic loci influence vaccine-induced protection against Mycobacterium tuberculosis in genetically diverse mice PLoS pathogens 2024-03-01 [PMID: 38452145] (Mouse)
• HG Roweth, MW Malloy, GJ Goreczny, IC Becker, Q Guo, EA Mittendorf, JE Italiano, SS McAllister, EM Battinelli Pro-inflammatory megakaryocyte gene expression in murine models of breast cancer Science Advances, 2022-10-12;8(41):eabo5224. 2022-10-12 [PMID: 36223471] (Mouse)
• CR Shaler, AA Parco, W Elhenawy, J Dourka, J Jury, EF Verdu, BK Coombes Psychological stress impairs IL22-driven protective gut mucosal immunity against colonising pathobionts Nature Communications, 2021-11-18;12(1):6664. 2021-11-18 [PMID: 34795263] (Mouse)
• M Abe, Y Kinjo, S Sadamoto, M Shinozaki, M Nagi, K Shibuya, Y Miyazaki alpha-galactosylceramide-stimulated invariant natural killer T-cells play a protective role in murine vulvovaginal candidiasis by Candida albicans PLoS ONE, 2021-11-16;16(11):e0259306. 2021-11-16 [PMID: 34784362] (Mouse)
• O Dusek, A Fajstova, A Klimova, P Svozilkova, T Hrncir, M Kverka, S Coufal, J Slemin, H Tlaskalova, JV Forrester, J Heissigero Severity of Experimental Autoimmune Uveitis Is Reduced by Pretreatment with Live Probiotic Escherichia coli Nissle 1917 Cells, 2020-12-25;10(1):. 2020-12-25 [PMID: 33375578] (Mouse)
• J Skupsky, S Sabui, M Hwang, M Nakasaki, MD Cahalan, HM Said Biotin Supplementation Ameliorates Murine Colitis by Preventing NF-kappaB Activation: Biotin supplementation ameliorates colitis Cell Mol Gastroenterol Hepatol, 2019-11-29;0(0):. 2019-11-29 [PMID: 31786364] (Mouse)
• EGD Hopkins, TI Roumelioti, C Mullineaux, JS Choudhary, G Frankel Intestinal Epithelial Cells and the Microbiome Undergo Swift Reprogramming at the Inception of Colonic Citrobacter rodentium Infection MBio, 2019-04-02;10(2):. 2019-04-02 [PMID: 30940698] (Mouse)
• H Mizobuchi, W Fujii, S Isokawa, K Ishizuka, Y Wang, S Watanabe, C Sanjoba, Y Matsumoto, Y Goto Exacerbation of hepatic injury during rodent malaria by myeloid-related protein 14 PLoS ONE, 2018-06-14;13(6):e0199111. 2018-06-14 [PMID: 29902248] (Mouse)
• M Eisenblaet, F Flores-Bor, JJ Lee, C Wefers, H Smith, R Hueting, MS Cooper, PJ Blower, D Patel, M Rodriguez-, H Milewicz, T Vogl, J Roth, A Tutt, T Schaeffter, T Ng Visualization of Tumor-Immune Interaction - Target-Specific Imaging of S100A8/A9 Reveals Pre-Metastatic Niche Establishment Theranostics, 2017-06-15;7(9):2392-2401. 2017-06-15 [PMID: 28744322] (Mouse)
• J Yano, MC Noverr, PL Fidel Vaginal Heparan Sulfate Linked to Neutrophil Dysfunction in the Acute Inflammatory Response Associated with Experimental Vulvovaginal Candidiasis MBio, 2017-03-14;8(2):. 2017-03-14 [PMID: 28292981] (Mouse)
• Nash E, Peters B, Lilly E, Noverr M, Fidel P A Murine Model of Candida glabrata Vaginitis Shows No Evidence of an Inflammatory Immunopathogenic Response. PLoS ONE, 2016-01-25;11(1):e0147969. 2016-01-25 [PMID: 26807975] (Mouse)
• Dulek D, Newcomb D, Goleniewska K, Cephus J, Zhou W, Reiss S, Toki S, Ye F, Zaynagetdinov R, Sherrill T, Blackwell T, Moore M, Boyd K, Kolls J, Peebles R Allergic airway inflammation decreases lung bacterial burden following acute Klebsiella pneumoniae infection in a neutrophil- and CCL8-dependent manner. Infect Immun, 2014-06-23;82(9):3723-39. 2014-06-23 [PMID: 24958709] (Mouse)

FAQs for S100A8 (DY3059). (Showing 1 - 2 of 2 FAQs).

1. Have any of your MRP8 antibodies been tested for use in neutralization, or blocking assays, on human samples?
   • Unfortunately at this time we have not tested, or received customer feedback on our MMP8 antibodies for use in blocking or neutralizing assays. If you were planning on testing any of our MMP8 antibodies for neutralizing or blocking capabilities, we would recommend our Innovator’s Reward Program. Under the terms of this program we would be happy to provide a credit for a free vial in exchange for new data on this previously untested or reported application. Please submit this data in the form of an online review. Additional Innovators Reward Program information can be found using this link.
2. We would like to stain paraffin embedded human intestinal tissue for Calprotectin. One question will be which cells express the majority of Calprotectin - invading neutrophils vs epithelium for example. The antibody list you provide is quite extensive. Which antibody would you recommend for our purpose? Which tissue would you recommend as positive control.
   • Nine of our antibodies to Calprotectin have been validated for IHC-P with human tissue, and you can see all of these products. A number of our IHC images were generated using human spleen samples, and as such this may be a good choice of a positive control tissue. We do sell a human spleen slide product, which is suitable for IHC-P. Unfortunately I do not have an in-depth knowledge of Calprotectin, however the image shown for our antibody with catalogue number NBP1-02826 demonstrates clear staining of neutrophils. The following paper also suggests that the protein is abundant in neutrophils: PMID 11435495.

Bioinformatics

• Gene Symbol: S100a8