Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse OPN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse OPN in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse OPN and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse OPN showed linear cur...ves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse OPN.
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• Specificity: Natural and recombinant mouse and rat OPN. This assay cross-reacts 8.2% with recombinant Human Osteopontin.
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Spp1

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

• BNSP
• Bone sialoprotein 1
• Eta-1
• MGC110940
• Nephropontin
• OPN
• Osteopontin
• secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
• secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
• Spp1
• SPP-1
• SPP1/CALPHA1 fusion
• Urinary stone protein
• uropontin

Background

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Osteopontin/OPN (MOST00)(46)

Publications using MOST00

• Vishwakarma, K;Bisht, A;Kumar, P;Kumar, S;Akhter, J;Payasi, A;Chaudhary, S;Aggarwal, A; Toxicokinetic Profiling of VRP-034: Evaluating its Potential in Mitigating Polymyxin B-Associated Nephrotoxicity International journal of antimicrobial agents 2024-11-27 [PMID: 39612992] (Rat)
• Costa, KLD;Abreu, LF;Tolomei, CB;Eleutério, RG;Basting, R;Balbinot, G;Collares, FM;Lopes, P;Veiga, N;Fernandes, GVO;Peruzzo, DC; Use of Local Melatonin with Xenogeneic Bone Graft to Treat Critical-Size Bone Defects in Rats with Osteoporosis: A Randomized Study Journal of functional biomaterials 2024-05-13 [PMID: 38786635] (Rat)
• Mamand, DR;Bazaz, S;Mohammad, DK;Saher, O;Wiklander, OPB;Sadeghi, B;Hassan, M;El-Andaloussi, S;Abedi-Valugerdi, M; Tumor cell derived osteopontin and prostaglandin E2 synergistically promote the expansion of myeloid derived suppressor cells during the tumor immune escape phase International immunopharmacology 2024-02-15 [PMID: 38364741] (Mouse)
• KG English, AL Reid, A Samani, GJF Coulis, SA Villalta, CJ Walker, S Tamir, MS Alexander Next-Generation SINE Compound KPT-8602 Ameliorates Dystrophic Pathology in Zebrafish and Mouse Models of DMD Biomedicines, 2022-09-26;10(10):. 2022-09-26 [PMID: 36289662] (Mouse)
• MJ Coyne, AE Schultze, DJ McCrann, RE Murphy, J Cross, M Strong-Tow, C Drake, R Mack Evaluation of renal injury and function biomarkers, including symmetric dimethylarginine (SDMA), in the rat passive Heymann nephritis (PHN) model PLoS ONE, 2022-05-27;17(5):e0269085. 2022-05-27 [PMID: 35622875] (Rat)
• O Mucha, K Kaziród, P Podkalicka, K Rusin, J Dulak, A ?oboda Dysregulated Autophagy and Mitophagy in a Mouse Model of Duchenne Muscular Dystrophy Remain Unchanged Following Heme Oxygenase-1 Knockout International Journal of Molecular Sciences, 2021-12-31;23(1):. 2021-12-31 [PMID: 35008897] (Mouse)
• X Jiang, F Zhang, X Ji, F Dong, H Yu, M Xue, Y Qiu, F Yang, X Hu, Z Bao Lipid-injured hepatocytes release sOPN to improve macrophage migration via CD44 engagement and pFak-NF&kappaB signaling Cytokine, 2021-02-27;142(0):155474. 2021-02-27 [PMID: 33647584] (Human, Mouse)
• K Ritter, K Jung, C Dolderer, D Appel, CC Oswald, U Ritz, MKE Schäfer Early Reciprocal Effects in a Murine Model of Traumatic Brain Injury and Femoral Fracture Mediators of Inflammation, 2021-01-18;2021(0):8835730. 2021-01-18 [PMID: 33531878] (Mouse)
• TA Pereira, G Vaz de Mel, E Trindade S, FE Pereira, M Maria de S Praziquantel pharmacotherapy reduces systemic osteopontin levels and liver collagen content in murine schistosomiasis mansoni International journal for parasitology, 2021-01-23;0(0):. 2021-01-23 [PMID: 33493521] (Mouse)
• A Frasca, Y Xue, AP Kossar, S Keeney, C Rock, A Zakharchen, M Streeter, RC Gorman, JB Grau, I George, JE Bavaria, A Krieger, DA Spiegel, RJ Levy, G Ferrari Glycation and Serum Albumin Infiltration Contribute to the Structural Degeneration of Bioprosthetic Heart Valves JACC Basic Transl Sci, 2020-08-05;5(8):755-766. 2020-08-05 [PMID: 32875167] (Rat)
• K Kohl, E Herzog, G Dickneite, S Pestel Evaluation of urinary biomarkers for early detection of acute kidney injury in a rat nephropathy model J Pharmacol Toxicol Methods, 2020-08-07;0(0):106901. 2020-08-07 [PMID: 32771565] (Rat)
• B Gómez-Sant, D Saenz de U, M Nuñez-Garc, F Gonzalez-R, X Buque, I Aurrekoetx, V Gutiérrez, MJ Gonzalez-R, C García-Mon, Á González-R, L Mosteiro, G Errazti, P Mifsut, S Gaztambide, L Castaño, C Martin, R Nogueiras, ML Martinez-C, WK Syn, P Aspichueta Liver osteopontin is required to prevent the progression of age-related nonalcoholic fatty liver disease Aging Cell, 2020-07-07;0(0):e13183. 2020-07-07 [PMID: 32638492] (Mouse)
• J Wilflingse, M Willi, HK Lee, H Olauson, J Jankowski, T Ichimura, R Erben, MT Valerius, L Hennighaus, JV Bonventre Enhancer and super-enhancer dynamics in repair after ischemic acute kidney injury Nat Commun, 2020-07-07;11(1):3383. 2020-07-07 [PMID: 32636391] (Mouse)
• PG Hitscheric, LH Xie, D Del Re, EJ Lee The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 2019-07-01;7(13):e14137. 2019-07-01 [PMID: 31301118] (Mouse)
• B Giri, VK Gupta, B Yaffe, S Modi, P Roy, V Sethi, SP Lavania, SM Vickers, V Dudeja, S Banerjee, J Watts, A Saluja Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia J Transl Med, 2019-05-20;17(1):163. 2019-05-20 [PMID: 31109340] (Human)
• E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou Osteopontin in the host response to Leishmania amazonensis BMC Microbiol., 2019-02-08;19(1):32. 2019-02-08 [PMID: 30736736] (Mouse)
• J Wang, J Huang, M Zhu, S Chen, C Chen, C Miao, H He, Y Song Osteopontin potentiates PM-induced IL-1? and IL-1? production via the ERK/JNK signaling pathway Ecotoxicol. Environ. Saf., 2019-01-09;171(0):467-474. 2019-01-09 [PMID: 30639873] (Mouse)
• JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion J. Clin. Invest., 2018-11-05;0(0):. 2018-11-05 [PMID: 30395540] (Mouse)
• MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression Exp. Mol. Med., 2018-11-05;50(11):142. 2018-11-05 [PMID: 30393382] (Mouse)
• E De Giglio, MA Bonifacio, AM Ferreira, S Cometa, ZY Ti, A Stanzione, K Dalgarno, P Gentile Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model Sci Rep, 2018-10-11;8(1):15130. 2018-10-11 [PMID: 30310164] (Mouse)
• M Nuñez-Garc, B Gomez-Sant, D Saenz de U, D Mestre, F Gonzalez-R, X Buque, V Gutiérrez-, ML Martinez-C, WK Syn, O Fresnedo, P Aspichueta Atorvastatin provides a new lipidome improving early regeneration after partial hepatectomy in osteopontin deficient mice Sci Rep, 2018-10-02;8(1):14626. 2018-10-02 [PMID: 30279550] (Mouse)
• M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018-04-25;0(0):. 2018-04-25 [PMID: 29695634] (Mouse)
• ZV Varga, K Erdelyi, J Paloczi, R Cinar, ZK Zsengeller, T Jourdan, C Matyas, NT Balazs, A Guillot, X Xiang, A Mehal, G Hasko, IE Stillman, S Rosen, B Gao, G Kunos, P Pacher Disruption of renal arginine metabolism promotes kidney injury in hepatorenal syndrome Hepatology, 2018-10-01;0(0):. 2018-10-01 [PMID: 29631342] (Mouse)
• LA O'Reilly, TL Putoczki, LA Mielke, JT Low, A Lin, A Preaudet, MJ Herold, K Yaprianto, L Tai, A Kueh, G Pacini, RL Ferrero, R Gugasyan, Y Hu, M Christie, S Wilcox, R Grumont, MDW Griffin, L O'Connor, GK Smyth, M Ernst, P Waring, S Gerondakis, A Strasser Loss of NF-?B1 Causes Gastric Cancer with Aberrant Inflammation and Expression of Immune Checkpoint Regulators in a STAT-1-Dependent Manner Immunity, 2018-03-20;48(3):570-583.e8. 2018-03-20 [PMID: 29562203] (Mouse)
• F Blanco, SE Heinonen, E Gurzeler, LM Berglund, AM Dutius And, O Kotova, AC Jönsson-Ry, S Ylä-Herttu, MF Gomez In vivo inhibition of nuclear factor of activated T-cells leads to atherosclerotic plaque regression in IGF-II/LDLR-/-ApoB100/100mice Diab Vasc Dis Res, 2018-03-02;0(0):1479164118759. 2018-03-02 [PMID: 29499628] (Mouse)
• N Wilck, L Markó, A Balogh, K Kräker, F Herse, H Bartolomae, IA Szijártó, M Gollasch, N Reichhart, O Strauss, A Heuser, D Brockschni, A Kretschmer, R Lesche, F Sohler, JP Stasch, P Sandner, FC Luft, DN Müller, R Dechend, N Haase Nitric oxide-sensitive guanylyl cyclase stimulation improves experimental heart failure with preserved ejection fraction JCI Insight, 2018-02-22;3(4):. 2018-02-22 [PMID: 29467337] (Rat)
• K Fu, Y Miyamoto, K Otake, K Sumi, E Saika, S Matsumura, N Sato, Y Ueno, S Seo, K Uno, SI Muramatsu, A Nitta Involvement of the accumbal osteopontin-interacting transmembrane protein 168 in methamphetamine-induced place preference and hyperlocomotion in mice Sci Rep, 2017-10-12;7(1):13084. 2017-10-12 [PMID: 29026117] (Mouse)
• A Aarup, CH Nielsen, LS Bisgaard, I Bot, HH El-Ali, A Kjaer, LB Nielsen, TX Pedersen Uremia does not affect neointima formation in mice Sci Rep, 2017-07-26;7(1):6496. 2017-07-26 [PMID: 28747676] (Mouse)
• GM Pio, Y Xia, MM Piaseczny, JE Chu, AL Allan Soluble bone-derived osteopontin promotes migration and stem-like behavior of breast cancer cells PLoS ONE, 2017-05-12;12(5):e0177640. 2017-05-12 [PMID: 28498874] (Mouse)
• J Grzesiak, A ?mieszek, K Marycz Ultrastructural changes during osteogenic differentiation in mesenchymal stromal cells cultured in alginate hydrogel Cell Biosci, 2017-01-03;7(0):2. 2017-01-03 [PMID: 28066541] (Rat)
• Fuquan Jin Deficient TSC1/TSC2-complex suppression of SOX9-osteopontin-AKT signalling cascade constrains tumour growth in tuberous sclerosis complex Hum. Mol. Genet, 2017-01-15;0(0):. 2017-01-15 [PMID: 28013293] (Mouse)
• The P2X4 receptor is required for neuroprotection via ischemic preconditioning Sci Rep, 2016-05-13;6(0):25893. 2016-05-13 [PMID: 27173846] (Mouse)
• Age-associated vascular inflammation promotes monocytosis during atherogenesis Aging Cell, 2016-05-02;0(0):. 2016-05-02 [PMID: 27135421] (Mouse)
• Huang H, Ma M High Sodium-Induced Oxidative Stress and Poor Anticrystallization Defense Aggravate Calcium Oxalate Crystal Formation in Rat Hyperoxaluric Kidneys. PLoS ONE, 2015-08-04;10(8):e0134764. 2015-08-04 [PMID: 26241473] (Rat)
• Zetterqvist A, Blanco F, Ohman J, Kotova O, Berglund L, de Frutos Garcia S, Al-Naemi R, Wigren M, McGuire P, Gonzalez Bosc L, Gomez M Nuclear factor of activated T cells is activated in the endothelium of retinal microvessels in diabetic mice. J Diabetes Res, 2015-03-31;2015(0):428473. 2015-03-31 [PMID: 25918731] (Mouse)
• Zelenchuk L, Hedge A, Rowe P PHEX mimetic (SPR4-peptide) corrects and improves HYP and wild type mice energy-metabolism. PLoS ONE, 2014-05-19;9(5):e97326. 2014-05-19 [PMID: 24839967] (Mouse)
• Chang S, Song S, Lee J, Yoon J, Park J, Choi S, Park J, Choi K, Choi C Phenotypic modulation of primary vascular smooth muscle cells by short-term culture on micropatterned substrate. PLoS ONE, 2014-02-04;9(2):e88089. 2014-02-04 [PMID: 24505388] (Rat)
• Brampton C, Aherrahrou Z, Chen L, Martin L, Bergen A, Gorgels T, Erdmann J, Schunkert H, Szabo Z, Varadi A, Le Saux O The level of hepatic ABCC6 expression determines the severity of calcification after cardiac injury. Am J Pathol, 2014-01-01;184(1):159-70. 2014-01-01 [PMID: 24479134] (Mouse)
• Chen J, Doroudi M, Cheung J, Grozier A, Schwartz Z, Boyan B Plasma membrane Pdia3 and VDR interact to elicit rapid responses to 1alpha,25(OH)(2)D(3). Cell Signal, 2013-07-27;25(12):2362-73. 2013-07-27 [PMID: 23896121] (Mouse)
• Shojaei F, Scott N, Kang X, Lappin PB, Fitzgerald AA, Karlicek S, Simmons BH, Wu A, Lee JH, Bergqvist S, Kraynov E Osteopontin induces growth of metastatic tumors in a preclinical model of non-small lung cancer. J. Exp. Clin. Cancer Res., 2012-03-23;31(0):26. 2012-03-23 [PMID: 22444159] (Mouse)
• Schupp N, Kolkhof P, Queisser N, Gartner S, Schmid U, Kretschmer A, Hartmann E, Oli RG, Schafer S, Stopper H Mineralocorticoid receptor-mediated DNA damage in kidneys of DOCA-salt hypertensive rats. FASEB J., 2010-12-06;25(3):968-78. 2010-12-06 [PMID: 21135038] (Rat)
• Chen J, Olivares-Navarrete R, Wang Y Protein-disulfide isomerase-associated 3 (Pdia3) mediates the membrane response to 1,25-dihydroxyvitamin D3 in osteoblasts. J. Biol. Chem., 2010-09-15;285(47):37041-50. 2010-09-15 [PMID: 20843786] (Mouse)
• Kiefer FW, Zeyda M, Gollinger K, Pfau B, Neuhofer A, Weichhart T, Saemann MD, Geyeregger R, Schlederer M, Kenner L, Stulnig TM Neutralization of osteopontin inhibits obesity-induced inflammation and insulin resistance. Diabetes, 2010-01-27;59(4):935-46. 2010-01-27 [PMID: 20107108] (Mouse)
• Takanashi M, Oikawa K, Sudo K, Tanaka M, Fujita K, Ishikawa A, Nakae S, Kaspar RL, Matsuzaki M, Kudo M, Kuroda M Therapeutic silencing of an endogenous gene by siRNA cream in an arthritis model mouse. Gene Ther., 2009-05-28;16(8):982-9. 2009-05-28 [PMID: 19474812] (Mouse)
• Shao J, Washington MK, Saxena R, Sheng H Heterozygous disruption of the PTEN promotes intestinal neoplasia in APCmin/+ mouse: roles of osteopontin. Carcinogenesis, 2007-08-11;28(12):2476-83. 2007-08-11 [PMID: 17693663] (Rat)
• Briscoe CP, Looper D, Tran P, Herrera J, McDonnell SR, Bhat BG LPS-induced biomarkers in mice: a potential model for identifying insulin sensitizers. Biochem. Biophys. Res. Commun., 2007-07-10;361(1):140-5. 2007-07-10 [PMID: 17659259] (Mouse)

FAQs for Osteopontin/OPN (MOST00). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Spp1