Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications ELISA
Conjugate
HRP

Order Details

Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit Summary

Background
The Quantikine Mouse OPN Immunoassay is a 4.5 hour solid-phase ELISA designed to measure mouse OPN in cell culture supernates, serum, plasma, and urine. It contains NS0-expressed recombinant mouse OPN and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural mouse OPN showed linear cur...ves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring mouse OPN.
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Specificity
Natural and recombinant mouse and rat OPN. This assay cross-reacts 8.2% with recombinant Human Osteopontin.
Assay Type
Solid Phase Sandwich ELISA
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
Spp1

Applications/Dilutions

Application Notes
No significant interference observed with available related molecules.
Publications
Read Publications using MOST00.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat Osteopontin (OPN) Quantikine ELISA Kit

  • BNSP
  • Bone sialoprotein 1
  • Eta-1
  • MGC110940
  • Nephropontin
  • OPN
  • Osteopontin
  • secreted phosphoprotein 1bone sialoprotein I, early T-lymphocyteactivation 1)
  • secreted phosphoprotein-1 (osteopontin, bone sialoprotein)
  • Spp1
  • SPP-1
  • SPP1/CALPHA1 fusion
  • Urinary stone protein
  • uropontin

Background

Osteopontin (OPN), also known as bone sialoprotein (BSP), is a secreted SIBLING family protein that can be variably modified by O- and N-glycosylation, sulfation, phosphorylation, and transglutamination. OPN is widely expressed and is prominent in mineralized tissues. It inhibits bone mineralization and kidney stone formation and promotes inflammation, cell ad¬hesion, and migration. Its expression is upregulat¬ed during inflammation, obesity, atherosclerosis, cancer, and tissue damage and contributes to the pathophysiology of these conditions. The central region of OPN contains RGD and non-RGD binding sites for multiple integrins. Adjacent to the RGD motif is the sequence SLAYGLR (SVVYGLR in human) which serves as a cryptic binding site for additional integrins: it is masked in full length OPN but is exposed following OPN cleavage by multiple proteases in tumors and sites of tissue injury.

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Species: Mu, Rt
Applications: ELISA
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Osteopontin/OPN (MOST00)(33)

We have publications tested in 3 confirmed species: Human, Mouse, Rat.


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Human
(1)
Mouse
(26)
Rat
(6)
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Showing Publications 1 - 10 of 33. Show All 33 Publications.
Publications using MOST00 Applications Species
PG Hitscheric, LH Xie, D Del Re, EJ Lee The effects of macrophages on cardiomyocyte calcium-handling function using in vitro culture models Physiol Rep, 2019;7(13):e14137. 2019 [PMID: 31301118] (Mouse) Mouse
B Giri, VK Gupta, B Yaffe, S Modi, P Roy, V Sethi, SP Lavania, SM Vickers, V Dudeja, S Banerjee, J Watts, A Saluja Pre-clinical evaluation of Minnelide as a therapy for acute myeloid leukemia J Transl Med, 2019;17(1):163. 2019 [PMID: 31109340] (Human) Human
E Giraud, E Rouault, L Fiette, JH Colle, D Smirlis, E Melanitou Osteopontin in the host response to Leishmania amazonensis BMC Microbiol., 2019;19(1):32. 2019 [PMID: 30736736] (Mouse) Mouse
J Wang, J Huang, M Zhu, S Chen, C Chen, C Miao, H He, Y Song Osteopontin potentiates PM-induced IL-1? and IL-1? production via the ERK/JNK signaling pathway Ecotoxicol. Environ. Saf., 2019;171(0):467-474. 2019 [PMID: 30639873] (Mouse) Mouse
JD Klement, AV Paschall, PS Redd, ML Ibrahim, C Lu, D Yang, E Celis, SI Abrams, K Ozato, K Liu An osteopontin/CD44 immune checkpoint controls CD8+ T cell activation and tumor immune evasion J. Clin. Invest., 2018;0(0):. 2018 [PMID: 30395540] (Mouse) Mouse
MN Lee, HS Hwang, SH Oh, A Roshanzade, JW Kim, JH Song, ES Kim, JT Koh Elevated extracellular calcium ions promote proliferation and migration of mesenchymal stem cells via increasing osteopontin expression Exp. Mol. Med., 2018;50(11):142. 2018 [PMID: 30393382] (Mouse) Mouse
E De Giglio, MA Bonifacio, AM Ferreira, S Cometa, ZY Ti, A Stanzione, K Dalgarno, P Gentile Multi-compartment scaffold fabricated via 3D-printing as in vitro co-culture osteogenic model Sci Rep, 2018;8(1):15130. 2018 [PMID: 30310164] (Mouse) Mouse
M Nuñez-Garc, B Gomez-Sant, D Saenz de U, D Mestre, F Gonzalez-R, X Buque, V Gutiérrez-, ML Martinez-C, WK Syn, O Fresnedo, P Aspichueta Atorvastatin provides a new lipidome improving early regeneration after partial hepatectomy in osteopontin deficient mice Sci Rep, 2018;8(1):14626. 2018 [PMID: 30279550] (Mouse) Mouse
M Mastri, CR Lee, A Tracz, RS Kerbel, M Dolan, Y Shi, JML Ebos Tumor-independent host secretomes induced by angiogenesis and immune-checkpoint inhibitors Mol. Cancer Ther., 2018;0(0):. 2018 [PMID: 29695634] (Mouse) Mouse
ZV Varga, K Erdelyi, J Paloczi, R Cinar, ZK Zsengeller, T Jourdan, C Matyas, NT Balazs, A Guillot, X Xiang, A Mehal, G Hasko, IE Stillman, S Rosen, B Gao, G Kunos, P Pacher Disruption of renal arginine metabolism promotes kidney injury in hepatorenal syndrome Hepatology, 2018;0(0):. 2018 [PMID: 29631342] (Mouse) Mouse
Show All 33 Publications.

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Product General Protocols

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FAQs for Osteopontin/OPN (MOST00). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP MOST00

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Bioinformatics

Gene Symbol Spp1