Mouse/Rat IL-33 Quantikine ELISA Kit

• Reactivity: Mu, Rt
• Applications: ELISA
• Conjugate: HRP

Mouse/Rat IL-33 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse/Rat IL-33 immunoassay is a 4.5 hour solid-phase ELISA designed to measure IL-33 in cell culture supernates, serum, and plasma. It contains E. coli-expressed recombinant mouse mature IL-33 and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate both recombinant mouse and rat IL-33. Results obtained using natu...ral IL-33 showed dose response curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural IL-33.
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• Specificity: Natural and recombinant mouse and rat IL-33
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il33

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse/Rat IL-33 Quantikine ELISA Kit

• C9orf26
• C9orf26chromosome 9 open reading frame 26 (NF-HEV)
• DKFZp586H0523
• DVS27
• DVS27-related protein
• IL1F11
• IL-1F11
• IL33
• IL-33
• interleukin 33
• Interleukin-1 family member 11
• interleukin-33
• NFHEV
• NF-HEV
• NF-HEVNFEHEV
• Nuclear factor from high endothelial venules
• RP11-575C20.2

Background

IL-33 (Interleukin-33) is released from physically damaged or necrotic cells. It triggers Th2-biased immune cell activation at sites of inflammation as well as regulatory T cell and M2 macrophage expansion. It additionally induces angiogenesis, promotes tumor cell migration and invasion, limits cardiac myocyte hypertrophy, and limits the development of atherosclerotic plaques. IL-33 signals through a receptor complex composed of ST2 and IL-1 RAcP. IL-1 RAcP also associates with IL-1 RI, IL-1 RII, IL-1 R6, and SCF R/c-kit. Soluble isoforms of ST2 and IL-1 RAcP function as decoy receptors that regulate IL-33 bioactivity.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-33 (M3300)(44)

Publications using M3300

• Woo, JH;Song, MK;Baek, SH;Park, CJ;Kim, JW;Kim, B;Lee, K; Polystyrene particles induces asthma-like Th2-mediated lung injury through IL-33 secretion Environment international 2025-09-08 [PMID: 40926454] (Mouse)
• Amuso, VM;Haas, MR;Cooper, PO;Chatterjee, R;Hafiz, S;Salameh, S;Gohel, C;Mazumder, MF;Josephson, V;Khorsandi, K;Horvath, A;Rahnavard, A;Shook, BA; Deep skin fibroblast-mediated macrophage recruitment supports acute wound healing bioRxiv : the preprint server for biology 2024-08-10 [PMID: 39149286] (Mouse)
• Roy, S;Roy, S;Halder, S;Jana, K;Ukil, A; Leishmania exploits host cAMP/EPAC/calcineurin signaling to induce an IL-33-mediated anti-inflammatory environment for the establishment of infection The Journal of biological chemistry 2024-05-13 [PMID: 38750790] (Mouse)
• Molfetta, R;Lecce, M;Milito, ND;Putro, E;Pietropaolo, G;Marangio, C;Scarno, G;Moretti, M;De Smaele, E;Santini, T;Bernardini, G;Sciumè, G;Santoni, A;Paolini, R; SCF and IL-33 regulate mouse mast cell phenotypic and functional plasticity supporting a pro-inflammatory microenvironment Cell death & disease 2023-09-20 [PMID: 37730723] (Mouse)
• Jin, J;Fan, YJ;Nguyen, TV;Yu, ZN;Song, CH;Lee, SY;Shin, HS;Chai, OH; Fallopia japonica Root Extract Ameliorates Ovalbumin-Induced Airway Inflammation in a CARAS Mouse Model by Modulating the IL-33/TSLP/NF-?B Signaling Pathway International journal of molecular sciences 2023-08-07 [PMID: 37569890] (Mouse)
• H Tallima, R El Ridi Increased hepatic interleukin-1, arachidonic acid, and reactive oxygen species mediate the protective potential of peptides shared by gut cysteine peptidases against Schistosoma mansoni infection in mice PloS Neglected Tropical Diseases, 2023-03-15;17(3):e0011164. 2023-03-15 [PMID: 36920999] (Mouse)
• HJ Park, RP Kataru, J Shin, GD Garc A Nor, EM Encarnacio, MG Klang, E Riedel, M Coriddi, JH Dayan, BJ Mehrara Keratinocytes coordinate inflammatory responses and regulate development of secondary lymphedema bioRxiv : the preprint server for biology, 2023-01-21;0(0):. 2023-01-21 [PMID: 36711669] (Mouse)
• D Jayawarden, S Priyamvada, T Kageyama, Z White, A Kumar, TF Griggs, A Majumder, R Akram, AN Anbazhagan, T Sano, PK Dudeja Loss of SLC26A3 Results in Colonic Mucosal Immune Dysregulation via Epithelial-Immune Cell Crosstalk Cellular and Molecular Gastroenterology and Hepatology, 2022-12-17;0(0):. 2022-12-17 [PMID: 36535508] (Mouse, Transgenic Mouse)
• KE Block, K Iijima, MJ Pierson, DA Walsh, R Tei, TA Kucaba, J Xu, MH Khan, C Staley, TS Griffith, HJ McSorley, H Kita, SC Jameson Physiological microbial exposure transiently inhibits mouse lung ILC2 responses to allergens Nature Immunology, 2022-11-21;0(0):. 2022-11-21 [PMID: 36411381] (Mouse)
• Y Lu, Y Huang, J Li, J Huang, L Zhang, J Feng, J Li, Q Xia, Q Zhao, L Huang, S Jiang, S Su Eosinophil extracellular traps drive asthma progression through neuro-immune signals Nature Cell Biology, 2021-10-06;23(10):1060-1072. 2021-10-06 [PMID: 34616019] (Mouse)
• QR Li, SR Tan, L Yang, W He, L Chen, FX Shen, Z Wang, HF Wang Mechanism of chlorogenic acid in alveolar macrophage polarization in Klebsiella pneumoniae-induced pneumonia Journal of leukocyte biology, 2021-09-29;0(0):. 2021-09-29 [PMID: 34585429] (Mouse)
• S Piñeiro-He, P Martínez, MA Blasco Short and dysfunctional telomeres protect from allergen-induced airway inflammation Aging Cell, 2021-05-04;20(5):e13352. 2021-05-04 [PMID: 33942458] (Mouse)
• Katherine M. Still, Samantha J. Batista, Carleigh A. O’Brien, Oyebola O. Oyesola, Simon P. Früh, Lauren M. Webb et al. Astrocytes promote a protective immune response to brain Toxoplasma gondii infection via IL-33-ST2 signaling PLOS Pathogens 2020-10-27 [PMID: 33108405] (Mouse)
• P Gunerka, K Gala, M Banach, J Dominowski, J Hucz-Kalit, K Mulewski, A Hajnal, EG Mikus, D Smuga, M Zagozda, K Dubiel, J Pieczykola, BM Zygmunt, M Wieczorek Preclinical characterization of CPL302-253, a selective inhibitor of PI3K&delta, as the candidate for the inhalatory treatment and prevention of Asthma PLoS ONE, 2020-07-23;15(7):e0236159. 2020-07-23 [PMID: 32702053] (Mouse)
• W Zhou, J Zhang, S Toki, K Goleniewsk, AE Norlander, DC Newcomb, P Wu, KL Boyd, H Kita, RS Peebles COX Inhibition Increases Alternaria-Induced Pulmonary Group 2 Innate Lymphoid Cell Responses and IL-33 Release in Mice J. Immunol., 2020-07-20;0(0):. 2020-07-20 [PMID: 32690653] (Mouse)
• K Takenaga, M Akimoto, N Koshikawa, H Nagase Cancer cell-derived interleukin-33 decoy receptor sST2 enhances orthotopic tumor growth in a murine pancreatic cancer model PLoS ONE, 2020-04-27;15(4):e0232230. 2020-04-27 [PMID: 32340025] (Mouse)
• BW Lewis, T Vo, I Choudhary, A Kidder, C Bathula, C Ehre, N Wakamatsu, S Patial, Y Saini Ablation of IL-33 Suppresses Th2 Responses but Is Accompanied by Sustained Mucus Obstruction in the Scnn1b Transgenic Mouse Model J. Immunol., 2020-02-14;0(0):. 2020-02-14 [PMID: 32060135] (Mouse)
• YC Kim, KH Kim, S Lee, JW Jo, JY Park, MS Park, B Tsogbadrak, JP Lee, JW Lee, DK Kim, KH Oh, IJ Jang, YS Kim, RH Cha, SH Yang ST2 blockade mitigates peritoneal fibrosis induced by TGF-beta and high glucose J. Cell. Mol. Med., 2019-08-09;23(10):6872-6884. 2019-08-09 [PMID: 31397957] (Human)
• C Morales De, JR Maxwell, MM Xu, A Menoret, P Mittal, N Tsurutani, AJ Adler, AT Vella Costimulation Induces CD4�T Cell Antitumor Immunity via an Innate-like Mechanism Cell Rep, 2019-04-30;27(5):1434-1445.e3. 2019-04-30 [PMID: 31042471] (Mouse)
• M Cottagiri, M Nyandjo, M Stephens, J Mantilla, H Saito, IR Mackay, NR Rose, DB Njoku In drug-induced, immune-mediated hepatitis, interleukin-33 reduces hepatitis and improves survival independently and as a consequence of FoxP3+ T-cell activity Cell. Mol. Immunol., 2018-07-20;0(0):. 2018-07-20 [PMID: 30030493] (Mouse)
• M Martin Jen, W Jia, AJ Schults, X Ye, GD Prestwich, S Oottamasat IL-33 mast cell axis is central in LL-37 induced bladder inflammation and pain in a murine interstitial cystitis model Cytokine, 2018-05-18;0(0):. 2018-05-18 [PMID: 29784508] (Mouse)
• AC Kohlgruber, ST Gal-Oz, NM LaMarche, M Shimazaki, D Duquette, HN Nguyen, AI Mina, T Paras, A Tavakkoli, U von Andria, AS Banks, T Shay, MB Brenner, L Lynch ?? T cells producing interleukin-17A regulate adipose regulatory T cell homeostasis and thermogenesis Nat. Immunol., 2018-04-18;19(5):464-474. 2018-04-18 [PMID: 29670241] (Mouse)
• SG Ha, M Dileepan, XN Ge, BN Kang, YG Greenberg, A Rao, G Muralidhar, L Medina-Kau, MA Thompson, CM Pabelick, SM O'Grady, SP Rao, P Sriramarao Knob protein enhances epithelial barrier integrity and attenuates airway inflammation J. Allergy Clin. Immunol., 2018-03-06;0(0):. 2018-03-06 [PMID: 29522849] (ELISA Capture, Mouse)
• I Nishioka, T Takai, N Maruyama, S Kamijo, P Suchiva, M Suzuki, S Kunimine, H Ochi, S Shimura, K Sudo, H Ogawa, K Okumura, S Ikeda Airway inflammation after epicutaneous sensitization of mice requires protease activity of low-dose allergen inhalation J. Allergy Clin. Immunol., 2018-02-06;0(0):. 2018-02-06 [PMID: 29426602] (Mouse)
• S Piñeiro-He, E Alfaro-Arn, JA Gregory, R Torrens, C Ruíz-Martí, M Adner, IP López, JG Pichel Characterization of the acute inflammatory profile and resolution of airway inflammation after Igf1r-gene targeting in a murine model of HDM-induced asthma PLoS ONE, 2017-12-22;12(12):e0190159. 2017-12-22 [PMID: 29272313] (Mouse)
• SK Chang, AC Kohlgruber, F Mizoguchi, X Michelet, BJ Wolf, K Wei, PY Lee, L Lynch, D Duquette, V Ceperuelo-, AS Banks, MB Brenner Stromal cell cadherin-11 regulates adipose tissue inflammation and diabetes J. Clin. Invest., 2017-07-31;0(0):. 2017-07-31 [PMID: 28758901] (Mouse)
• Diego Martin-Sanchez, Olga Ruiz-Andres, Jonay Poveda, Susana Carrasco, Pablo Cannata-Ortiz, Maria D. Sanchez-Niño et al. Ferroptosis, but Not Necroptosis, Is Important in Nephrotoxic Folic Acid–Induced AKI Journal of the American Society of Nephrology 2017-01-01 [PMID: 27352622] (Mouse)
• The PDGF-BB-SOX7 axis-modulated IL-33 in pericytes and stromal cells promotes metastasis through tumour-associated macrophages Nat Commun, 2016-05-06;7(0):11385. 2016-05-06 [PMID: 27150562] (Mouse)
• Yang S, Lin J, Wu S, Huang K, Jung F, Ma M, Wang Hsu G, Jow G Membrane translocation of IL-33 receptor in ventilator induced lung injury. PLoS ONE, 2015-03-27;10(3):e0121391. 2015-03-27 [PMID: 25815839] (Rat)
• Tancevski I, Nairz M, Duwensee K, Auer K, Schroll A, Heim C, Feistritzer C, Hoefer J, Gerner R, Moschen A, Heller I, Pallweber P, Li X, Theurl M, Demetz E, Wolf A, Wolf D, Eller P, Ritsch A, Weiss G Fibrates ameliorate the course of bacterial sepsis by promoting neutrophil recruitment via CXCR2. EMBO Mol Med, 2014-04-06;6(6):810-20. 2014-04-06 [PMID: 24755316] (Mouse)
• Hara K, Iijima K, Elias M, Seno S, Tojima I, Kobayashi T, Kephart G, Kurabayashi M, Kita H Airway uric acid is a sensor of inhaled protease allergens and initiates type 2 immune responses in respiratory mucosa. J Immunol, 2014-03-24;192(9):4032-42. 2014-03-24 [PMID: 24663677] (Mouse)
• Lin J, Sun B, Jiang C, Hong H, Zheng Y Sirt2 suppresses inflammatory responses in collagen-induced arthritis. Biochem Biophys Res Commun, 2013-11-06;441(4):897-903. 2013-11-06 [PMID: 24211200] (Mouse)
• Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5). J Bone Miner Res, 2013-10-01;28(10):2070-80. 2013-10-01 [PMID: 23553711] (Mouse)
• Luo H, Higuchi K, Matsumoto K, Mori M An interleukin-33 gene polymorphism is a modifier for eosinophilia in rats. Genes Immun, 2013-02-28;14(3):192-7. 2013-02-28 [PMID: 23446743] (Rat)
• Hardman C, Panova V, McKenzie A IL-33 citrine reporter mice reveal the temporal and spatial expression of IL-33 during allergic lung inflammation. Eur J Immunol, 2012-12-26;43(2):488-98. 2012-12-26 [PMID: 23169007] (Mouse)
• Bartemes KR, Iijima K, Kobayashi T, Kephart GM, McKenzie AN, Kita H IL-33-responsive lineage- CD25+ CD44(hi) lymphoid cells mediate innate type 2 immunity and allergic inflammation in the lungs. J. Immunol., 2011-12-23;188(3):1503-13. 2011-12-23 [PMID: 22198948] (Mouse)
• Taube C, Tertilt C, Gyulveszi G, Dehzad N, Kreymborg K, Schneeweiss K, Michel E, Reuter S, Renauld JC, Arnold-Schild D, Schild H, Buhl R, Becher B IL-22 Is Produced by Innate Lymphoid Cells and Limits Inflammation in Allergic Airway Disease. PLoS ONE, 2011-07-18;6(7):e21799. 2011-07-18 [PMID: 21789181] (Mouse)
• Besnard AG, Togbe D, Guillou N, Erard F, Quesniaux V, Ryffel B IL-33-activated dendritic cells are critical for allergic airway inflammation. Eur. J. Immunol., 2011-05-25;41(6):1675-86. 2011-05-25 [PMID: 21469105] (Mouse)
• Nelson MP, Christmann BS, Werner JL, Metz AE, Trevor JL, Lowell CA, Steele C IL-33 and M2a alveolar macrophages promote lung defense against the atypical fungal pathogen Pneumocystis murina. J. Immunol., 2011-01-10;186(4):2372-81. 2011-01-10 [PMID: 21220696] (Mouse)
• Kim W, Kim J, Shin C, Kim H, Doh Y, Cho HR, Kwon B A novel method for procuring a large quantity of mature murine eosinophils in vivo. J. Immunol. Methods, 2010-10-01;363(1):90-4. 2010-10-01 [PMID: 20888335] (Mouse)
• Hsu CL, Neilsen CV, Bryce PJ IL-33 is produced by mast cells and regulates IgE-dependent inflammation. PLoS ONE, 2010-08-03;5(8):e11944. 2010-08-03 [PMID: 20689814] (Mouse)
• Kaldunski M, Jia S, Geoffrey R, Basken J, Prosser S, Kansra S, Mordes JP, Lernmark A, Wang X, Hessner MJ Identification of a serum-induced transcriptional signature associated with type 1 diabetes in the BioBreeding rat. Diabetes, 2010-08-03;59(10):2375-85. 2010-08-03 [PMID: 20682698] (Rat)
• Shimosato T, Fujimoto M, Tohno M, Sato T, Tateo M, Otani H, Kitazawa H CpG oligodeoxynucleotides induce strong up-regulation of interleukin 33 via Toll-like receptor 9. Biochem. Biophys. Res. Commun., 2010-02-21;394(1):81-6. 2010-02-21 [PMID: 20178773] (Mouse)
• Hazlett LD, McClellan SA, Barrett RP, Huang X, Zhang Y, Wu M, Van Rooijen N, Szliter E IL-33 shifts macrophage polarization, promoting resistance against Pseudomonas aeruginosa keratitis. Invest. Ophthalmol. Vis. Sci., 2009-11-05;51(3):1524-32. 2009-11-05 [PMID: 19892870] (Mouse)

FAQs for IL-33 (M3300). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il33