Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse OPG Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse OPG in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse OPG and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate recombinant mouse OPG accurately. Results obtained using natural mouse OPG showed dose response curv...es that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse OPG.
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• Specificity: Natural and recombinant mouse OPG
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Tnfrsf11b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: Interference observed with 1 or more available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse Osteoprotegerin/TNFRSF11B Quantikine ELISA Kit

• OCIF
• OCIFMGC29565
• OPG
• OPGtumor necrosis factor receptor superfamily member 11B
• Osteoclastogenesis inhibitory factor
• Osteoprotegerin
• TNFRSF11B
• TR1
• tumor necrosis factor receptor superfamily, member 11b

Background

Osteoprotegerin (OPG), also known as OCIF and TNFRSF11B, is a secreted TNF superfamily receptor that regulates bone density. OPG is widely expressed and constitutively released as a homodimer by mesenchymal stem cells, fibroblasts, and endothelial cells. OPG functions as a decoy receptor for TRANCE/RANK L and TRAIL. OPG regulates the cell surface expression of TRANCE and its ability to stimulate osteoclastogenesis. Dysregulation of OPG/TRANCE interactions can lead to juvenile Paget’s disease, osteoporosis, bone loss, and vascular calcification.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for Osteoprotegerin/TNFRSF11B (MOP00)(57)

Publications using MOP00

• Uehara, N;Shibusawa, N;Mikami, Y;Kyumoto-Nakamura, Y;Sonoda, S;Kato, H;Yamaza, T;Kukita, T; Bone metastatic mammary tumor cell-derived extracellular vesicles inhibit osteoblast maturation via JNK signaling Archives of biochemistry and biophysics 2023-12-01 [PMID: 37979903] (Mouse)
• E Cho, X Che, MJ Ang, S Cheon, J Lee, KS Kim, CH Lee, SY Lee, HY Yang, C Moon, C Park, JY Choi, TH Lee Peroxiredoxin 5 regulates osteogenic differentiation through interaction with hnRNPK during bone regeneration Elife, 2023-02-03;12(0):. 2023-02-03 [PMID: 36735291] (Mouse)
• C Polan, C Brenner, M Herten, G Hilken, F Grabellus, HL Meyer, M Burggraf, M Dudda, W Jahnen-Dec, C Wedemeyer, MD Kauther Increased UHMWPE Particle-Induced Osteolysis in Fetuin-A-Deficient Mice Journal of functional biomaterials, 2023-01-04;14(1):. 2023-01-04 [PMID: 36662077] (Mouse)
• J Yong, S Gröger, J von Bremen, S Ruf Ciliary Neurotrophic Factor (CNTF) Inhibits In Vitro Cementoblast Mineralization and Induces Autophagy, in Part by STAT3/ERK Commitment International Journal of Molecular Sciences, 2022-08-18;23(16):. 2022-08-18 [PMID: 36012576] (Mouse)
• JJ Wu, ZL Sun, SY Liu, ZH Chen, ZD Yuan, ML Zou, YY Teng, YY Li, DY Guo, FL Yuan The ASIC3-M-CSF-M2 macrophage-positive feedback loop modulates fibroblast-to-myofibroblast differentiation in skin fibrosis pathogenesis Cell Death & Disease, 2022-06-06;13(6):527. 2022-06-06 [PMID: 35661105] (Human)
• M Smeda, KM Galler, M Woelflick, A Rosendahl, C Moehle, B Lenhardt, W Buchalla, M Widbiller Molecular Biological Comparison of Dental Pulp- and Apical Papilla-Derived Stem Cells International Journal of Molecular Sciences, 2022-02-27;23(5):. 2022-02-27 [PMID: 35269758] (Human)
• JJM Junqueira, JD Lourenço, KR da Silva, V Jorgetti, RP Vieira, AA de Araujo, K De Angelis, AT Correia, LHV Alves, IFLC Tibério, AP Barbosa, FDTQDS Lopes Increased bone resorption by long-term cigarette smoke exposure in animal model Heliyon, 2021-12-10;7(12):e08587. 2021-12-10 [PMID: 34977408] (Mouse)
• W Zhao, P Wiedemann, EM Wölfel, M Neven, S Peters, T Imhof, M Koch, B Busse, M Amling, T Schinke, TA Yorgan Decreased Trabecular Bone Mass in Col22a1-Deficient Mice Cells, 2021-11-04;10(11):. 2021-11-04 [PMID: 34831244] (Mouse)
• N Liao, T Koehne, J Tuckermann, I Triviai, M Amling, JP David, T Schinke, J Luther Osteoblast-specific inactivation of p53 results in locally increased bone formation PLoS ONE, 2021-11-18;16(11):e0249894. 2021-11-18 [PMID: 34793446] (Mouse)
• EJ Lee, W Na, MK Kang, YH Kim, DY Kim, H Oh, SI Kim, SY Oh, S Park, K Park, YH Kang Hydroxycoumarin Scopoletin Inhibits Bone Loss through Enhancing Induction of Bone Turnover Markers in a Mouse Model of Type 2 Diabetes Biomedicines, 2021-06-07;9(6):. 2021-06-07 [PMID: 34200167] (Mouse)
• W Li, C Wei, L Xu, B Yu, Y Chen, D Lu, L Zhang, X Song, L Dong, S Zhou, Z Xu, J Zhu, X Chen, C Su Schistosome infection promotes osteoclast-mediated bone loss PloS Pathogens, 2021-03-18;17(3):e1009462. 2021-03-18 [PMID: 33735306] (Mouse)
• M Timmen, H Hidding, M Götte, TE Khassawna, D Kronenberg, R Stange The heparan sulfate proteoglycan Syndecan-1 influences local bone cell communication via the RANKL/OPG axis Sci Rep, 2020-11-25;10(1):20510. 2020-11-25 [PMID: 33239699] (Mouse)
• MA Hannappe, L Arnould, A Méloux, B Mouhat, F Bichat, M Zeller, Y Cottin, C Binquet, C Vergely, C Creuzot-Ga, C Guenancia Vascular density with optical coherence tomography angiography and systemic biomarkers in low and high cardiovascular risk patients Sci Rep, 2020-10-07;10(1):16718. 2020-10-07 [PMID: 33028913] (Human)
• KM Cawley, NC Bustamante, AG Guha, RS MacLeod, J Xiong, I Gubrij, Y Liu, R Mulkey, M Palmieri, JD Thostenson, JJ Goellner, CA O'Brien Local Production of Osteoprotegerin by Osteoblasts Suppresses Bone Resorption Cell Rep, 2020-09-08;32(10):108052. 2020-09-08 [PMID: 32905775] (Mouse)
• M Tsukasaki, T Asano, R Muro, NC Huynh, N Komatsu, K Okamoto, K Nakano, T Okamura, T Nitta, H Takayanagi OPG Production Matters Where It Happened Cell Rep, 2020-09-08;32(10):108124. 2020-09-08 [PMID: 32905763] (Mouse)
• T Velletri, Y Huang, Y Wang, Q Li, M Hu, N Xie, Q Yang, X Chen, Q Chen, P Shou, Y Gan, E Candi, AP Margherita, M Agostini, H Yang, G Melino, Y Shi, Y Wang Loss of p53 in mesenchymal stem cells promotes alteration of bone remodeling through negative regulation of osteoprotegerin Cell Death Differ., 2020-07-21;0(0):. 2020-07-21 [PMID: 32694652] (Mouse)
• K Sahbani, CP Cardozo, WA Bauman, HA Tawfeek Abaloparatide exhibits greater osteoanabolic response and higher cAMP stimulation and beta-arrestin recruitment than teriparatide Physiol Rep, 2019-10-01;7(19):e14225. 2019-10-01 [PMID: 31565870] (Mouse)
• T Heckt, LJ Brylka, M Neven, M Amling, T Schinke Deficiency of sphingosine-1-phosphate receptor 3 does not affect the skeletal phenotype of mice lacking sphingosine-1-phosphate lyase PLoS ONE, 2019-07-17;14(7):e0219734. 2019-07-17 [PMID: 31314788] (Mouse)
• J Kang, YJ Choi, BY Seo, U Jo, SI Park, YH Kim, KH Park A Selective FGFR inhibitor AZD4547 suppresses RANKL/M-CSF/OPG-dependent ostoclastogenesis and breast cancer growth in the metastatic bone microenvironment Sci Rep, 2019-06-19;9(1):8726. 2019-06-19 [PMID: 31217507] (Mouse)
• AE Pesaro, M Katz, M Liberman, C Pereira, CLP Mangueira, AEZ de Carvalh, KS Carvalho, CH Nomura, M Franken, CV Serrano Circulating osteogenic proteins are associated with coronary artery calcification and increase after myocardial infarction PLoS ONE, 2018-08-23;13(8):e0202738. 2018-08-23 [PMID: 30138356] (Mouse)
• SM Zimmerman, ME Heard-Lips, M Dimori, JD Thostenson, EM Mannen, CA O'Brien, R Morello Loss of RANKL in osteocytes dramatically increases cancellous bone mass in the osteogenesis imperfecta mouse (oim) Bone Rep, 2018-07-02;9(0):61-73. 2018-07-02 [PMID: 30105276] (Mouse)
• J Xiong, K Cawley, M Piemontese, Y Fujiwara, H Zhao, JJ Goellner, CA O'Brien Soluble RANKL contributes to osteoclast formation in adult mice but not ovariectomy-induced bone loss Nat Commun, 2018-07-25;9(1):2909. 2018-07-25 [PMID: 30046091] (Mouse)
• NJ Lee, IM Clarke, RF Enriquez, V Nagy, J Penninger, PA Baldock, H Herzog Central RANK signalling in NPY neurons alters bone mass in male mice Neuropeptides, 2018-02-15;0(0):. 2018-02-15 [PMID: 29477253] (Mouse)
• N Yang, B Baban, CM Isales, XM Shi Role of glucocorticoid-induced leucine zipper (GILZ) in inflammatory bone loss PLoS ONE, 2017-08-03;12(8):e0181133. 2017-08-03 [PMID: 28771604] (Mouse)
• PA Hulley, T Bishop, A Vernet, JE Schneider, JR Edwards, NA Athanasou, HJ Knowles Hypoxia-Inducible Factor 1-alpha does not regulate osteoclastogenesis but enhances bone resorption activity via prolyl-4-hydroxylase 2 J. Pathol., 2017-05-29;0(0):. 2017-05-29 [PMID: 28418093] (Mouse)
• OJ Park, J Kim, J Yang, CH Yun, SH Han Muramyl Dipeptide, a Shared Structural Motif of Peptidoglycans, Is a Novel Inducer of Bone Formation through Induction of Runx2 J. Bone Miner. Res, 2017-04-27;0(0):. 2017-04-27 [PMID: 28337794] (Mouse)
• HM Davis, R Pacheco-Co, EG Atkinson, LR Brun, AR Gortazar, J Harris, M Hiasa, SA Bolarinwa, T Yoneda, M Ivan, A Bruzzaniti, T Bellido, LI Plotkin Disruption of the Cx43/miR21 pathway leads to osteocyte apoptosis and increased osteoclastogenesis with aging Aging Cell, 2017-03-19;0(0):. 2017-03-19 [PMID: 28317237] (Mouse)
• Silicon Nitride: A Synthetic Mineral for Vertebrate Biology Sci Rep, 2016-08-19;6(0):31717. 2016-08-19 [PMID: 27539146] (Human)
• Deletion of FoxO1, 3 and 4 in Osteoblast Progenitors Attenuates the Loss of Cancellous Bone Mass in a Mouse Model of Type 1 Diabetes J Bone Miner Res, 2016-09-07;0(0):. 2016-09-07 [PMID: 27491024] (Mouse)
• Kassem A, Henning P, Lundberg P, Souza P, Lindholm C, Lerner U Porphyromonas gingivalis Stimulates Bone Resorption by Enhancing RANKL (Receptor Activator of NF-kappaB Ligand) through Activation of Toll-like Receptor 2 in Osteoblasts. J Biol Chem, 2015-06-17;290(33):20147-58. 2015-06-17 [PMID: 26085099] (Mouse)
• Maruyama K, Fukasaka M, Uematsu S, Takeuchi O, Kondo T, Saitoh T, Martino M, Akira S 5-Azacytidine-induced protein 2 (AZI2) regulates bone mass by fine-tuning osteoclast survival. J Biol Chem, 2015-02-17;290(15):9377-86. 2015-02-17 [PMID: 25691576] (Mouse)
• Baek , Jong Min, Kim , Ju-Young, Cheon , Yoon-Hee, Park , Sun-Hyan, Ahn , Sung-Jun, Yoon , Kwon-Ha, Oh , Jaemin, Lee , Myeung S Aconitum pseudo-laeve var. erectum inhibits receptor activator of nuclear factor kappa-B ligand-induced osteoclastogenesis via the c-Fos/nuclear factor of activated T-cells, cytoplasmic 1 signaling pathway and prevents lipopolysaccharide-induced bone loss in mice. Molecules, 2014-08-05;19(8):11628-44. 2014-08-05 [PMID: 25100255] (Mouse)
• Teufel S, Grotsch B, Luther J, Derer A, Schinke T, Amling M, Schett G, Mielenz D, David J Inhibition of bone remodeling in young mice by bisphosphonate displaces the plasma cell niche into the spleen. J Immunol, 2014-06-04;193(1):223-33. 2014-06-04 [PMID: 24899506] (Mouse)
• Yoo Y, Kwag J, Kim K, Kim C Effects of neuropeptides and mechanical loading on bone cell resorption in vitro. Int J Mol Sci, 2014-04-08;15(4):5874-83. 2014-04-08 [PMID: 24717410] (Mouse)
• Hayashida C, Ito J, Nakayachi M, Okayasu M, Ohyama Y, Hakeda Y, Sato T Osteocytes produce interferon-beta as a negative regulator of osteoclastogenesis. J Biol Chem, 2014-03-07;289(16):11545-55. 2014-03-07 [PMID: 24610813] (Mouse)
• Ohba T, Cole H, Cates J, Slosky D, Haro H, Ando T, Schwartz H, Schoenecker J Bisphosphonates inhibit osteosarcoma-mediated osteolysis via attenuation of tumor expression of MCP-1 and RANKL. J Bone Miner Res, 2014-06-01;29(6):1431-45. 2014-06-01 [PMID: 24443409] (Mouse)
• Wintges K, Beil F, Albers J, Jeschke A, Schweizer M, Claass B, Tiegs G, Amling M, Schinke T Impaired bone formation and increased osteoclastogenesis in mice lacking chemokine (C-C motif) ligand 5 (Ccl5). J Bone Miner Res, 2013-10-01;28(10):2070-80. 2013-10-01 [PMID: 23553711] (Mouse)
• Gao L, Faibish D, Fredman G, Herrera B, Chiang N, Serhan C, Van Dyke T, Gyurko R Resolvin E1 and chemokine-like receptor 1 mediate bone preservation. J Immunol, 2012-12-14;190(2):689-94. 2012-12-14 [PMID: 23241890] (Mouse)
• Buckle , Clive He, De Leenheer , Evy, Lawson , Michelle, Yong , Kwee, Rabin , Neil, Perry , Mark, Vanderkerken , Karen, Croucher , Peter Ia Soluble rank ligand produced by myeloma cells causes generalised bone loss in multiple myeloma. PLoS ONE, 2012-08-29;7(8):e41127. 2012-08-29 [PMID: 22952578] (Mouse)
• Hayashi M, Nakashima T, Taniguchi M, Kodama T, Kumanogoh A, Takayanagi H Osteoprotection by semaphorin 3A. Nature, 2012-05-03;485(7396):69-74. 2012-05-03 [PMID: 22522930] (Mouse)
• Ma B, Zhang Q, Wu D, Wang YL, Hu YY, Cheng YP, Yang ZD, Zheng YY, Ying HJ Strontium fructose 1,6-diphosphate prevents bone loss in a rat model of postmenopausal osteoporosis via the OPG/RANKL/RANK pathway. Acta Pharmacol. Sin., 2012-03-19;33(4):479-89. 2012-03-19 [PMID: 22426695] (Rat)
• Onal M, Galli C, Fu Q, Xiong J, Weinstein RS, Manolagas SC, O'Brien CA The RANKL distal control region is required for the increase in RANKL expression, but not the bone loss, associated with hyperparathyroidism or lactation in adult mice. Mol. Endocrinol., 2011-12-29;26(2):341-8. 2011-12-29 [PMID: 22207718] (Mouse)
• Ho TY, Santora K, Chen JC, Frankshun AL, Bagnell CA Effects of relaxin and estrogens on bone remodeling markers, receptor activator of NF-kB ligand (RANKL) and osteoprotegerin (OPG), in rat adjuvant-induced arthritis. Bone, 2011-03-16;48(6):1346-53. 2011-03-16 [PMID: 21419242] (Rat)
• Sample SJ, Collins RJ, Wilson AP, Racette MA, Behan M, Markel MD, Kalscheur VL, Hao Z, Muir P Systemic effects of ulna loading in male rats during functional adaptation. J. Bone Miner. Res., 2010-09-01;25(9):2016-28. 2010-09-01 [PMID: 20499374] (Rat)
• Neunaber C, Catala-Lehnen P, Beil FT Increased trabecular bone formation in mice lacking the growth factor midkine. J. Bone Miner. Res., 2010-08-01;25(8):1724-35. 2010-08-01 [PMID: 20200993] (Mouse)
• Sauer AV, Mrak E, Hernandez RJ, Zacchi E, Cavani F, Casiraghi M, Grunebaum E, Roifman CM, Cervi MC, Ambrosi A, Carlucci F, Roncarolo MG, Villa A, Rubinacci A, Aiuti A ADA-deficient SCID is associated with a specific microenvironment and bone phenotype characterized by RANKL/OPG imbalance and osteoblast insufficiency. Blood, 2009-07-24;114(15):3216-26. 2009-07-24 [PMID: 19633200] (Mouse)
• O'Connor RD, Zayzafoon M, Farach-Carson MC, Schanen NC Mecp2 deficiency decreases bone formation and reduces bone volume in a rodent model of Rett syndrome. Bone, 2009-05-03;45(2):346-56. 2009-05-03 [PMID: 19414073] (Mouse)
• Pierroz DD, Rufo A, Bianchi EN, Glatt V, Capulli M, Rucci N, Cavat F, Rizzoli R, Teti A, Bouxsein ML, Ferrari SL Beta-Arrestin2 regulates RANKL and ephrins gene expression in response to bone remodeling in mice. J. Bone Miner. Res., 2009-05-01;24(5):775-84. 2009-05-01 [PMID: 19113915] (Mouse)
• Qiang YW, Chen Y, Stephens O, Brown N, Chen B, Epstein J, Barlogie B, Shaughnessy JD Myeloma-derived Dickkopf-1 disrupts Wnt-regulated osteoprotegerin and RANKL production by osteoblasts: a potential mechanism underlying osteolytic bone lesions in multiple myeloma. Blood, 2008-02-27;112(1):196-207. 2008-02-27 [PMID: 18305214] (Mouse)
• Morony S, Tintut Y, Zhang Z, Cattley RC, Van G, Dwyer D, Stolina M, Kostenuik PJ, Demer LL Osteoprotegerin inhibits vascular calcification without affecting atherosclerosis in ldlr(-/-) mice. Circulation, 2008-01-02;117(3):411-20. 2008-01-02 [PMID: 18172035] (Mouse)
• Secchiero P, Corallini F, Rimondi E, Chiaruttini C, di Iasio MG, Rustighi A, Del Sal G, Zauli G Activation of the p53 pathway down-regulates the osteoprotegerin expression and release by vascular endothelial cells. Blood, 2007-11-13;111(3):1287-94. 2007-11-13 [PMID: 18000166] (Mouse)
• Zhu J, Jia X, Xiao G, Kang Y, Partridge NC, Qin L EGF-like ligands stimulate osteoclastogenesis by regulating expression of osteoclast regulatory factors by osteoblasts: implications for osteolytic bone metastases. J. Biol. Chem., 2007-07-17;282(37):26656-64. 2007-07-17 [PMID: 17636266] (Mouse)
• Li Y, Toraldo G, Li A, Yang X, Zhang H, Qian WP, Weitzmann MN B cells and T cells are critical for the preservation of bone homeostasis and attainment of peak bone mass in vivo. Blood, 2007-01-03;109(9):3839-48. 2007-01-03 [PMID: 17202317] (Mouse)
• Nakamichi Y, Udagawa N, Kobayashi Y, Nakamura M, Yamamoto Y, Yamashita T, Mizoguchi T, Sato M, Mogi M, Penninger JM, Takahashi N Osteoprotegerin reduces the serum level of receptor activator of NF-kappaB ligand derived from osteoblasts. J. Immunol., 2007-01-01;178(1):192-200. 2007-01-01 [PMID: 17182555] (Mouse)
• Berry JE, Ealba EL, Pettway GJ, Datta NS, Swanson EC, Somerman MJ, McCauley LK JunB as a downstream mediator of PTHrP actions in cementoblasts. J. Bone Miner. Res., 2005-11-07;21(2):246-57. 2005-11-07 [PMID: 16418780] (Mouse)
• Zehnder AF, Kristiansen AG, Adams JC, Merchant SN, McKenna MJ Osteoprotegerin in the inner ear may inhibit bone remodeling in the otic capsule. Laryngoscope, 2005-01-01;115(1):172-7. 2005-01-01 [PMID: 15630389] (Mouse)
• Lubberts E, van den Bersselaar L, Oppers-Walgreen B, Schwarzenberger P, Coenen-de Roo CJ, Kolls JK, Joosten LA, van den Berg WB IL-17 promotes bone erosion in murine collagen-induced arthritis through loss of the receptor activator of NF-kappa B ligand/osteoprotegerin balance. J. Immunol., 2003-03-01;170(5):2655-62. 2003-03-01 [PMID: 12594294] (Mouse)

Bioinformatics

• Gene Symbol: Tnfrsf11b