Mouse MFG-E8 Quantikine ELISA Kit

• Reactivity: Mu
• Applications: ELISA
• Conjugate: HRP

Mouse MFG-E8 Quantikine ELISA Kit Summary

• Background: The Quantikine Mouse MFG-E8 Immunoassay is a 4.5 hour solid phase ELISA designed to measure mouse MFG-E8 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant mouse MFG-E8 and antibodies raised against the recombinant factor. This immunoassay has been shown to quantitate the recombinant factor accurately. Results obtained using natural mouse MFG-E8 showed dose-...response curves that were parallel to the standard curves obtained using the recombinant kit standards. These results indicate that this kit can be used to determine relative mass values for natural mouse MFG-E8.
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• Specificity: Natural and recombinant mouse MFG-E8
• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Mfge8

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse MFG-E8 Quantikine ELISA Kit

• BA46
• Breast epithelial antigen BA46
• EDIL1
• hP47
• HsT19888
• lactadherin
• Lactahedrin
• Medin
• MFG1
• MFGE8
• MFG-E8
• MFGM
• milk fat globule-EGF factor 8 protein
• Milk fat globule-EGF factor 8
• O-acetyl disialoganglioside synthase
• OAcGD3S
• SED1
• SPAG10
• sperm associated antigen 10
• sperm surface protein hP47

Background

MFG-E8, originally identified as a product of lactating mammary glands, is also secreted by activated macrophages and immature dendritic cells. MFG-E8 functions as an opsonin by binding phosphatidylserine on apoptotic cells and enhancing their phagocytosis.

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for MFG-E8 (MFGE80)(9)

Publications using MFGE80

• Sokolova, D;Ghansah, SA;Puletti, F;Georgiades, T;De Schepper, S;Zheng, Y;Crowley, G;Wu, L;Rueda-Carrasco, J;Koutsiouroumpa, A;Muckett, P;Freeman, OJ;Khakh, BS;Hong, S; Astrocyte-derived MFG-E8 facilitates microglial synapse elimination in Alzheimer's disease mouse models bioRxiv : the preprint server for biology 2024-09-01 [PMID: 39257734] (Transgenic Mouse)
• Chaung, W;Zhang, F;Ma, G;Yen, H;Jacob, A;Brenner, M;Wang, P; Development of human cell-expressed tag-free rhMFG-E8 as a radiation mitigator and a therapeutic for acute kidney injury Research square 2023-05-15 [PMID: 37293000] (Rat)
• D Han, D Lu, S Huang, J Pang, Y Wu, J Hu, X Zhang, Y Pi, G Zhang, J Wang Small extracellular vesicles from Ptpn1-deficient macrophages alleviate intestinal inflammation by reprogramming macrophage polarization via lactadherin enrichment Redox Biology, 2022-11-28;58(0):102558. 2022-11-28 [PMID: 36462232] (Mouse, Transgenic Mouse)
• M Ikemoto-Ue, H Zhou, T Kurosawa, Y Yoshimoto, M Toyoda, N Kanazawa, T Nakazawa, M Morita, K Tsuchida, A Uezumi Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation Aging Cell, 2021-12-24;0(0):e13536. 2021-12-24 [PMID: 34953020] (Mouse)
• Y Lu, L Liu, J Pan, B Luo, H Zeng, Y Shao, H Zhang, H Guan, D Guo, C Zeng, R Zhang, X Bai, H Zhang, D Cai MFG-E8 regulated by miR-99b-5p protects against osteoarthritis by targeting chondrocyte senescence and macrophage reprogramming via the NF-&kappaB pathway Cell Death & Disease, 2021-05-25;12(6):533. 2021-05-25 [PMID: 34031369] (Mouse)
• HF Bu, S Subramania, H Geng, X Wang, F Liu, PM Chou, C Du, IG De Plaen, XD Tan MFG-E8 Plays an Important Role in Attenuating Cerulein-Induced Acute Pancreatitis in Mice Cells, 2021-03-25;10(4):. 2021-03-25 [PMID: 33806041] (Mouse)
• J Brelstaff, AM Tolkovsky, B Ghetti, M Goedert, MG Spillantin Living Neurons with Tau Filaments Aberrantly Expose Phosphatidylserine and Are Phagocytosed by Microglia Cell Rep, 2018-08-21;24(8):1939-1948.e4. 2018-08-21 [PMID: 30134156] (Mouse)
• Uchiyama A, Yamada K, Ogino S, Yokoyama Y, Takeuchi Y, Udey M, Ishikawa O, Motegi S MFG-E8 regulates angiogenesis in cutaneous wound healing. Am J Pathol, 2014-05-15;184(7):1981-90. 2014-05-15 [PMID: 24838098] (Mouse)
• The neuroprotective effects of milk fat globule-EGF factor 8 against oligomeric amyloid beta toxicity. J Neuroinflammation, 2012-06-28;9(0):148. 2012-06-28 [PMID: 22742657] (Mouse)

FAQs for MFG-E8 (MFGE80). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Mfge8